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Time-resolved fluorescence immunoassay kit for detecting novel coronavirus and preparation method thereof

A time-resolved fluorescence and coronavirus technology, applied in the analysis of materials, fluorescence/phosphorescence, biological material analysis, etc., can solve the problems of high requirements for detection equipment, poor repeatability, and many interference factors, and achieve convenient and specific detection operations Strong and sensitive effect

Pending Publication Date: 2020-07-21
李翀
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The methods used to detect the new coronavirus COVID-19 mainly include RT-PCR nucleic acid detection and other methods, but in the application of these methods, the requirements for detection equipment are high, the detection cost is high; there are many interference factors, the repeatability is not good, and the detection time is long

Method used

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  • Time-resolved fluorescence immunoassay kit for detecting novel coronavirus and preparation method thereof
  • Time-resolved fluorescence immunoassay kit for detecting novel coronavirus and preparation method thereof
  • Time-resolved fluorescence immunoassay kit for detecting novel coronavirus and preparation method thereof

Examples

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Effect test

Embodiment 1

[0043] Example 1. Preparation of Time-Resolved Fluorescence Immunochromatography Kit for Detecting Novel Coronavirus

[0044] Time-resolved fluorescent immunochromatography kit for the detection of novel coronavirus COVID-19. The test strip adopts the principle of double-antibody sandwich immunochromatography to detect the new coronavirus in human samples (respiratory tract, blood, cornea, stool, etc.) Content of the virus COVID-19. The kit consists of a time-resolved fluorescent immunochromatographic test strip for detecting the novel coronavirus COVID-19, a sample buffer and an ID card containing the standard curve of the novel coronavirus COVID-19.

[0045] Specifically, the test strip of the present invention is as figure 1 and figure 2 As shown, the sample filter pad 2, microsphere marking pad 3, detection pad 5, and water-absorbing pad 4 are sequentially overlapped on the PVC bottom plate 1; The COVID-19 monoclonal antibody coating; the test pad 3 has a test line (T)...

Embodiment 2

[0059] Example 2, Time-resolved fluorescence immunochromatography kit for quantitative detection of novel coronavirus

[0060] Add different concentrations of human novel coronavirus COVID-19 antigen calibrator (70ng / ml, 35ng / ml, 14ng / ml, 7ng / ml, 3.5ng / ml, 0.7ng / ml to the test strip prepared according to Example 1. ml, 0.0ng / ml), a total of seven concentrations, and each concentration set three repetitions, all of which were prepared by diluting 0.5mg / ml human novel coronavirus COVID-19N protein recombinant antigen with sample buffer, after 15min of chromatography, passed The dry fluorescent immunoassay analyzer reads the fluorescence intensity ratio of the detection line and the quality control line, wherein the fluorescence intensity ratio=detection value of the detection line / detection value of the quality control line.

[0061] Take the concentration of the new coronavirus COVID-19 calibrator as the X-axis, and take the fluorescence intensity of the sample detection line / q...

Embodiment 3

[0072] Example 3, Time-resolved fluorescence immunochromatography kit for qualitative detection of novel coronavirus

[0073] Prepare test strips according to Example 1 of the present invention, and set four different concentrations of human novel coronavirus COVID-19 antigen calibrator: 70ng / ml, 7ng / ml, 0.7ng / ml, 0.0ng / ml.

[0074] Follow the steps below to perform a qualitative test:

[0075] (1) Put the sample to be tested and the detection reagent to room temperature, and wait for the temperature to balance;

[0076] (2) Take 100 μl of the sample to be tested and add it to 100 μl of sample buffer, mix well to obtain a mixed sample;

[0077] (3) Take 100 μl of the above-mentioned mixed sample, add it to the sample hole of the test strip, and react at room temperature for 10-15 minutes in the dark;

[0078] (4) Turn on the portable ultraviolet detection pen, irradiate the detection test strip through the exposed part of the detection window, observe the fluorescent display...

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Abstract

The invention discloses a time-resolved fluorescence immunoassay kit for detecting novel coronavirus and a preparation method thereof. The kit comprises a test strip and a fluorescence immunoassay analyzer. The test strip comprises a bottom plate, and a sample filter pad, a microsphere marking pad, a detection pad and a water absorption pad which are located on the bottom plate. One end of the detection pad is laminated with one end of the microsphere marking pad, the other end of the detection pad is laminated with one end of the absorbent paper, and one end of the sample filter pad is laminated with the other end of the microsphere marking pad. Qualitative and quantitative detection of the novel coronavirus COVID-19 in various human body samples can be realized, and aiming at different application scenes and requirements, small-batch and easy-to-operate rapid early screening by primary communities and high-throughput and high-accuracy clinical diagnosis by a central hospital can be met at the same time.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a time-resolved fluorescence immunochromatography kit for detecting novel coronavirus and a preparation method thereof. Background technique [0002] The new type of coronavirus belongs to the genus Coronaviridae β, has an envelope, and the particles are round or oval, often pleomorphic, with a diameter of 60-140nm. Its genetic characteristics are significantly different from SARSr-CoV and MERSr-Cov, and its homology with bat SARS-like coronavirus (bat-SL-CoVZC45) is more than 85%. On January 12, 2020, the World Health Organization tentatively named it "2019-nCoV" (2019 Novel Coronavirus). On February 11, WHO Director-General Tedros Adhanom Ghebreyesus announced at the press conference of the Global Research and Innovation Forum that the new coronavirus was officially named "COVID-19", where "Co" stands for "coronavirus" and "Vi " stands for "virus" and "D" stands ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N21/64
CPCG01N33/56983G01N33/558G01N21/6408G01N2333/165
Inventor 李翀
Owner 李翀
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