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Time-resolved fluorescence immunochromatographic assay kit for hepatitis B5 tests

A technology of time-resolved fluorescence and immunochromatography, which is applied in the field of immune analysis of infectious disease proteins, can solve the problems of complex test operation, fuzzy determination of test results, slow response speed, etc., and achieve the effect of convenient and fast operation

Inactive Publication Date: 2019-04-12
BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing detection methods have some disadvantages, such as long detection time and complicated test operation
Although ordinary colloidal gold immunochromatography is easy to operate, there will be problems such as slow detection reaction speed, uneven color development, fuzzy determination of detection results, and poor sensitivity.

Method used

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  • Time-resolved fluorescence immunochromatographic assay kit for hepatitis B5 tests
  • Time-resolved fluorescence immunochromatographic assay kit for hepatitis B5 tests
  • Time-resolved fluorescence immunochromatographic assay kit for hepatitis B5 tests

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Embodiment Construction

[0042] Unless otherwise defined, the technical terms used in the following embodiments have the same meaning as commonly understood by those skilled in the art to which the present invention belongs. The test reagents used in the following examples, unless otherwise specified, are conventional biochemical reagents; the experimental methods, unless otherwise specified, are conventional methods.

[0043] All numerical designations, such as pH, temperature, time, concentration, including ranges, are approximate. It is to be understood that, although not always expressly stated, all numerical designations are preceded by the term "about". It is also to be understood that, although not always explicitly stated, the reagents described herein are merely exemplary and equivalents are known in the art.

[0044] The present invention will be described in detail below in conjunction with examples.

[0045] 1. Preparation of test card

[0046] (1) Preparation of detection area and qual...

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Abstract

The invention provides a time-resolved fluorescence immunochromatographic assay kit for hepatitis B5 tests. The time-resolved fluorescence immunochromatographic assay kit comprises detecting cards andfluorescent microsphere working fluid; the detecting cards comprise the detecting card used for detecting HBsAg, the detecting card used for detecting HBeAg, the detecting card used for detecting anti-HBs, the detecting card used for detecting anti-HBe, and the detecting card used for detecting anti-HBc; and the fluorescent microsphere working fluid contains time-resolved fluorescent microspheresfor marking the corresponding antigen or the corresponding antibody. In the detecting process, by combining fluorescent microsphere compounds in a detecting area and a quality control area, under theeffect of exciting light, emitted fluorescence is collected and processed by a fluorescence immunoassay analyzer, and thus the detecting result can be calculated. The time-resolved fluorescence immunochromatographic assay kit has the advantages of high sensitivity, high specificity, easy and convenient operation and accurate measuring.

Description

technical field [0001] The invention belongs to the technical field of infectious disease protein immune analysis, and in particular relates to a hepatitis B five-item time-resolved fluorescence immunochromatographic assay kit and a preparation method thereof. Background technique [0002] Hepatitis B virus (HBV) is a small virus that belongs to the group of hepatotropic deoxyribonucleic acid (DNA) viruses. The virus particle is composed of two parts, the outer membrane and the inner core. The complete HBV particle is a spherical particle with a diameter of 42nm, and its outer membrane is 7nm thick. symbols of. Hepatitis B surface antibody (HBsAb) is a protective antibody against hepatitis B virus immunity. Its appearance marks the specific immunity to HBV infection, and usually occurs during the recovery period of HBV infection or after injection of hepatitis B vaccine. [0003] The diameter of the central part of the virus is about 28nm, which is the core of the virus, ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/576
CPCG01N33/5761G01N33/5764G01N33/68
Inventor 刘萍栾大伟刘朝阳朱伶张晴晴
Owner BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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