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Method for detecting concentration of substance to be detected, fluorescence immunoassay analyzer and storage medium

A technology of fluorescence immunochromatography and analytes, which is applied in the direction of material analysis, material analysis, image analysis, etc. through optical means, and can solve problems such as large errors, low detection accuracy, and inaccurate positioning of fluorescent regions.

Inactive Publication Date: 2020-11-03
GENRUI BIOTECH INC
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, for the image analysis algorithm of the chromatography test paper, there are problems such as inaccurate positioning of the fluorescent area and rough de-baseline processing, so the detection accuracy is low and the error is large

Method used

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  • Method for detecting concentration of substance to be detected, fluorescence immunoassay analyzer and storage medium
  • Method for detecting concentration of substance to be detected, fluorescence immunoassay analyzer and storage medium
  • Method for detecting concentration of substance to be detected, fluorescence immunoassay analyzer and storage medium

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Embodiment Construction

[0097] The present invention will be described in detail below in conjunction with specific embodiments. The following examples will help those skilled in the art to further understand the present invention, but do not limit the present invention in any form. It should be noted that those skilled in the art can make several modifications and improvements without departing from the concept of the present invention. These all belong to the protection scope of the present invention.

[0098] In order to make the purpose, technical solution and advantages of the present application clearer, the present application will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present application, not to limit the present application.

[0099] It should be noted that, if there is no conflict, various features in the embodiments of the present...

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Abstract

The embodiment of the invention relates to the field of immunochromatography detection, and discloses a method for detecting the concentration of a substance to be detected, a fluorescence immunoassayanalyzer and a storage medium, and the method comprises the steps: carrying out the first image processing of a fluorescence image, so as to remove non-fluorescence regions at two sides of the chromatography direction of the fluorescence image, and obtaining a first fluorescence image; and on the basis of the first fluorescence image, removing the non-fluorescence regions on the two sides of thevertical chromatography direction to obtain a second fluorescence image, so that the interference of the non-fluorescence regions on the two sides of the vertical chromatography direction on subsequent image analysis and calculation can be avoided, namely, the interference on subsequent concentration calculation is reduced. Namely, the non-fluorescent region is removed step by step twice, so thatthe fluorescent region can be accurately positioned, and the concentration detection accuracy is high.

Description

technical field [0001] The embodiments of the present invention relate to the field of immunochromatography detection, and in particular to a method for detecting the concentration of analyte, a fluorescent immunoassay analyzer and a storage medium. Background technique [0002] Immunochromatography is an emerging immunoassay technology based on chromatography and antigen-antibody specific immune response. Immunochromatography uses a strip-shaped fiber chromatographic material fixed with a detection line (T line) and a control line (C line) as a chromatographic test paper. The detection sample moves on the chromatographic test paper through capillary action, and during the movement, the analyte in the detection sample combines with the fluorescently labeled primary antibody to form a structure of analyte-first antibody-fluorescein, and continues to move forward , when passing through the T-line of the NC membrane, the analyte in the structure of the analyte-first antibody-f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/558G06T5/00G06T7/00
CPCG01N21/6428G01N33/558G06T7/97G06T5/70
Inventor 林贵文伍海军吴华强
Owner GENRUI BIOTECH INC
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