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Method for calibrating inter-platform difference of fluorescence immunoassay analyzer

A technology of fluorescence immunoassay and inter-stage difference, which is applied in the direction of material analysis, material analysis, fluorescence/phosphorescence, etc. through optical means, which can solve the problems of large correction deviation, large deviation, narrow application range, etc., and achieve the solution of excessive correction deviation Large, low calibration deviation, wide application range effect

Inactive Publication Date: 2021-03-09
SINOCARE
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Problems solved by technology

However, the above-mentioned existing calibration method has too large deviation when calibrating the signal intensity of the low-value interval, that is, it cannot be applied to the detection and calibration of the low-value interval at all. Therefore, the scope of application of this calibration method is relatively narrow. The correction deviation is too large, resulting in very inaccurate final detection results
[0005] Therefore, the existing technology has a large room for improvement

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  • Method for calibrating inter-platform difference of fluorescence immunoassay analyzer
  • Method for calibrating inter-platform difference of fluorescence immunoassay analyzer

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Embodiment 1

[0024] A method for calibrating differences between fluorescent immunoassay instruments, comprising the following steps:

[0025] (1) Prepare 6 samples of different concentrations, numbered K1-K6, test each sample on the testing machine and the standard machine respectively, and obtain 6 test values ​​T and 6 nominal values ​​S, that is, there are 6 pairs of arrays (T, S); Described test value T, nominal value S are the average value that the sample of single concentration obtains after testing machine, standard machine test 3 times respectively;

[0026] (2) The 6 pairs of arrays (T, S) obtained in step (1) are calculated according to the logarithmic formula: L T = log 10 (T), L S = log 10 (S) perform logarithmic operations respectively to obtain 6 pairs of new data (L T , L S ); the specific test and calculation results are shown in Table 1:

[0027] Table 1

[0028] sample number Nominal value S Test value T L S

L T

K1 0.0216 0.0205 -1.665...

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Abstract

The invention relates to the technical field of fluorescence immunoassay and particularly relates to a method for calibrating inter-platform difference of a fluorescence immunoassay analyzer. The method comprises steps of performing logarithm operation processing on n pairs of arrays obtained by testing of a testing machine and a standard machine, then performing curve fitting to obtain a corresponding fitting curve function, substituting actual measurement data logarithm operation processing into the fitting curve function to obtain a corresponding value during actual testing, and finally performing power multiplication calculation to obtain a calibrated numerical value. By means of the calibration method, no matter when the fluorescence signal intensity value is low or when the fluorescence signal intensity value is high, deviation of the obtained calibrated value is within 3%, and calibration deviation is extremely low; the method can be well suitable for common low-value interval fluorescence immunoassay indexes, is wide in application range, enables a final detection result to be more accurate, improves the detection accuracy of an instrument, and effectively solves a problemthat a conventional calibration method is too large in correction deviation when the intensity value of a fluorescence signal is low.

Description

technical field [0001] The invention relates to the technical field of fluorescence immunoassay, and more specifically, relates to a method for calibrating differences between fluorescence immunoassay instruments. Background technique [0002] Fluorescence immunoassay analyzers are usually used in the industry for fluorescence immunoassay detection. Due to the instrument’s own factors such as light sources, there are certain differences in the test results between the instruments that leave the factory. Therefore, it is necessary to calibrate the test results to solve the problem of fluorescence immunoassay analyzers. The stage difference. [0003] The existing calibration method is to use a set of quality control strips to test the fluorescence intensity values ​​(or ratios) respectively on the fluorescent immunoassay analyzer and the standard machine to be shipped from the factory, and record them as measured values ​​and nominal values ​​respectively. The nominal value i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00G01N33/53G01N33/58G01N21/64
CPCG01N35/00693G01N35/00G01N33/5302G01N33/582G01N21/64G01N2035/00702G01N2021/6417
Inventor 张胜军汤四媛罗继全李昆鹏
Owner SINOCARE
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