Decellularized black carp skin biomimetic substrate for regeneration restoration as well as preparation method and application thereof

A decellularized herring technology, applied in the field of decellularized herring skin biomimetic matrix for regenerative repair and its preparation, can solve the problems of delaying conventional wound healing and no degreasing treatment, so as to improve biological safety and compatibility, cell ablation The effect of high removal rate and avoiding religious barriers

Pending Publication Date: 2018-09-04
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Kerecis TM The salmon used is a cold-water marine fish with extremely high fat content. There is no special degreasing treatment in its preparation process. Although its rich in omega-3 and other unsaturated fatty acids can promote the healing of refractory wounds, it delays the healing of conventional wounds

Method used

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  • Decellularized black carp skin biomimetic substrate for regeneration restoration as well as preparation method and application thereof
  • Decellularized black carp skin biomimetic substrate for regeneration restoration as well as preparation method and application thereof
  • Decellularized black carp skin biomimetic substrate for regeneration restoration as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preparation of decellularized herring skin biomimetic matrix for regenerative repair

[0042] (1) Pretreatment of herring skin: fresh herring skin is scraped off residual scales and redundant muscle tissues, rinsed with deionized water at 4°C several times to remove residual blood; cut into small pieces of 30×50mm according to different anatomical parts, and quickly Freeze at -40°C, take out and thaw under running water at room temperature before use;

[0043] (2) Remove mucous membrane / liquid and degrease: at 4°C, stir with 3.5% NaCl solution with a material-to-liquid ratio of 1:20 (w / w) for 1 hour, then remove the waste liquid, and roughly clean the herring skin to remove part of the redundancy; above The steps are repeated 3 times;

[0044] (3) Microstructure protection: at 4°C, stir with PBS buffer solution (0.1M, pH7.4) with a solid-to-liquid ratio of 1:20 (w / w) for 2 hours and then remove the waste liquid; repeat the above steps twice;

[0045] (4) Cle...

Embodiment 2

[0052] Example 2 Preparation of decellularized herring skin biomimetic matrix for regenerative repair

[0053] (1) herring skin pretreatment: with embodiment 1.

[0054] (2) Remove mucous membrane / liquid and degrease: at 4°C, stir with 5% NaCl solution with a material-to-liquid ratio of 1:10 (w / w) for 1 hour, then remove the waste liquid, and roughly clean the herring skin to remove part of the redundancy; above The steps are repeated 2 times.

[0055] (3) Microstructure protection: at 4°C, stir with PBS buffer solution (0.2M, pH7.0) with a solid-to-liquid ratio of 1:10 (w / w) for 2 hours, then remove the waste liquid; repeat the above steps once.

[0056] (4) Cleaning: at 4°C, stir for 4 hours with a 5% NaCl solution with a solid-to-liquid ratio of 1:10 (w / w), and then remove the waste liquid.

[0057] (5) Initial decellularization treatment: at 4°C, 2% sodium deoxycholate (containing 50 μg / ml gentamicin sulfate) with a solid-liquid ratio of 1:10 (w / w) was stirred for 18 hou...

Embodiment 3

[0063] Example 3 Preparation of Decellularized Herring Skin Biomimetic Matrix for Regeneration and Repair

[0064] (1) herring skin pretreatment: with embodiment 1;

[0065] (2) remove mucous membrane / fluid and degrease: with embodiment 2;

[0066] (3) Microstructure protection: at 4°C, stir for 2 hours with D-Hank’s buffer solution (pH 7.2) with a solid-to-liquid ratio of 1:10 (w / w), and then remove the waste liquid; repeat the above steps twice.

[0067] (4) Cleaning: at 4°C, stir with a 4% NaCl solution with a material-to-liquid ratio of 1:10 (w / w) for 4 hours and then remove the waste liquid;

[0068] (5) Initial decellularization treatment: at 4°C, 0.5% sodium dodecylsulfonate (containing gentamicin sulfate 50 μg / ml) with a solid-liquid ratio of 1:10 (w / w) was stirred for 12 hours. The herring skin was rearranged to remove part of the redundant tissue formed in the above treatment process; at 4°C, it was washed 3 times with Hank's buffer solution with a solid-to-liquid ...

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Abstract

The invention discloses a decellularized black carp skin biomimetic substrate for regeneration restoration as well as a preparation method and application thereof. The decellularized black carp skin biomimetic substrate for regeneration restoration is obtained by using black carp skin as raw materials through special treatment. The preparation method of the decellularized black carp skin biomimetic substrate for regeneration restoration comprises the process steps of raw material pretreatment, coarse finishing, degreasing, decellularizing, re-finishing, storage liquid pretreatment, shaping andthe like. The decellularized black carp skin biomimetic substrate for regeneration restoration prepared by the invention has the advantages that the substrate cell removal is thorough; the natural porous network structure is complete; the material is the biomimetic cell outer substrate support frame material with low immunogenicity; good micro environment can be provided for the tissue regeneration restoration and cell growth. Compared with common use decellularized substrate products from pigs, cows and the like, the decellularized black carp skin biomimetic substrate prepared by the invention has the advantages that the zoonosis virus carrying and spreading risk is low; the religious ethic risk is low; the resources are rich; the cost is low.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a decellularized herring skin bionic matrix for regeneration and repair, a preparation method and application thereof. Background technique [0002] Wound regeneration has always been a medical problem that many clinical departments urgently need to solve. Although with the improvement of medical theory and technological progress, many new wound repair products have emerged and been applied, but it is still difficult to meet the growing clinical needs. To date, the problem of trauma repair is still one of the main factors leading to disability and even death. [0003] At present, the commonly used wound repair products in clinic mainly include active device type, wet healing type and biological type. Among them, biological wound repair products have been used because of their biological activity close to the physiological structure or function of the body and meet ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/60
CPCA61L27/362A61L27/3687A61L27/60A61L2430/10A61L2430/34
Inventor 位晓娟张长青张伟
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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