47 results about "Bovine embryo" patented technology

The invention discloses a novel bovine embryo transplantation gun capable of bending and extending forward along with the uterine horn, and relates to the field of embryo transplantation guns, the novel bovine embryo transplantation gun comprises a gun barrel, a gun core and a culture solution thin tube, the culture solution thin tube is installed in a barrel hole in the inner end of the gun barrel in a matched mode, a piston is arranged in the culture solution thin tube, and the gun core is arranged in the gun barrel. According to the invention, an embryo in the slim tube is pushed into the extension catheter through the piston, and then the piston is punctured through the hollow steel needle to be communicated with the inner cavity of the slim tube; an operator pushes a piston handle of the injector to enable gas in the injector to enter the extension catheter through the hollow steel needle, and an embryo in the extension catheter is driven into the uterine horn of a cattle by the gas, so that the problem that the embryo cannot be driven into the uterus by a gun core after a slim tube is connected with the extension catheter is solved, and the operator does not need to grab the front end of the uterine horn; the operation difficulty is greatly reduced, the use of operators is facilitated, and the conception rate is indirectly improved.

The invention relates to a method for gender identification of early bovine embryos, comprising the following steps: performing in vitro fertilization of oocytes and sperm to obtain morulae; cutting the morulae in the segmentation liquid droplet with a segmentation knife to obtain embryonic tissues containing 10 to 12 cells As a sample for gender identification; the ring-mediated isothermal amplification method gender identification kit makes samples and prepares Master Mix; after the samples are mixed, put the reaction tube in the ring-mediated isothermal amplification real-time turbidity detection system for reaction, and determine the sample according to the results One of the three cases needs to be redone for male, female, uncertain. The method of the invention presents excellent technical effects as described in the specification.

The present invention relates to a convenient sex determination method of bovine embryos, the identification rate of the blood genome achieves 100%, and the identification rate of the embryo sex exceeds 99.0%. This method has convenient operation, high sensitivity, good practicability and simple equipment, and can determine the embryo sex in 1 hour. The formed detection kit of embryo sex can be applied to production absolutely and the cost is only 1 / 10 of products of the same kind. In the present invention 6 specific primers are designed according to the bovine specific sequences and male specific sequences, and 6 specific sites can be recognized. Large amount of DNA fragments of different sizes are amplified in a short time using a dumbbell-shaped structure unit as a core that is formed by the target sequences. The only requirement is aqueous thermostat at 60-65DEG C. dNTPs precipitation is used in the determination of the result (The negative result is that a white band appears in the middle part of the reaction tube).

The invention relates to a new method for cloning embryo with bovine sperms, which comprises the steps as follows: (1) a collected bovine ovary is cleaned, and an ovarium mound-oocyte complex is drawn out for in vitro culture to lead the oocyte to be cultured and developed to a second meiosis metaphase; (2) then dyeing and fluorescence localization are carried out on the nucleolus of the oocyte,and the nucleolus is denucleated to obtain the denucleated bovine oocyte; (3) the semen of an excellent breeding bull is collected or the commercial semen of a self-sex controlled excellent breeding bull is selected and stored in a liquid nitrogen container; (4) the semen is unfrozen and capacitation treatment is carried out on the semen; then the semen is arranged in the injecta of the oocyte; (5) two capacitation sperms are injected into the cytoplast of the denucleated bovine oocyte to obtain a sperm reconstructed embryo; (6) the sperm reconstructed embryo is activated and cultured. The invention overcomes the single mode that the donor cell in the traditional nuclear transfer can only be a somatocyte, and is a new method for generating a bovine embryo by using double sperms to replacethe somatocyte as a donor to be injected into the cytoplast of denucleated bovine oocyte.

The invention relates to a method for bovine in-vitro fertilized embryo culture and a used culture solution. Particularly, on one hand, the invention relates to a method for culturing a bovine in-vitro fertilized embryo. The method comprises the following steps of putting the bovine in-vitro fertilized embryo into a bovine in-vitro fertilized embryo culture solution and carrying out embryo in-vitro culture under the conditions that the temperature is 38.5 DEG C, the content of CO2 is 0.5% and the humidity is 100%. The invention further relates to a bovine in-vitro fertilized embryo culture solution. The culture solution comprises NaCl, KCl, NaHCO3, MgCl2, KH2PO3, sodium pyruvate, glucose, calcium galactonate, fetal calf serum, L-glutamine, an essential amino-acid, a nonessential amino acid, glutathione and water. The method and the used culture solution have excellent technical effects in the specification, for example, the method has a higher blastocyst hatching rate when the bovine in-vitro fertilized embryo is cultured.