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Determination method by liquid chromatography-electrospray mass spectrometry for detecting various phthalate esters in milk simultaneously

A mass spectrometry detection and liquid state technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of insufficient sensitivity and selectivity, and achieve high sensitivity, good accuracy and precision, and reliable specificity and confirmation Effect

Inactive Publication Date: 2009-10-07
JIANGNAN UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Early methods include titration, colorimetry, spectrophotometry, thin-layer chromatography, etc., but they are not sensitive enough and the selectivity is not good enough.

Method used

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  • Determination method by liquid chromatography-electrospray mass spectrometry for detecting various phthalate esters in milk simultaneously
  • Determination method by liquid chromatography-electrospray mass spectrometry for detecting various phthalate esters in milk simultaneously
  • Determination method by liquid chromatography-electrospray mass spectrometry for detecting various phthalate esters in milk simultaneously

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] 1 material

[0036] 1.1 Main reagents and materials

[0037] Methanol Chromatographically Pure Merk

[0038] Acetonitrile Chromatographic grade Merk

[0039] Trichloroacetic acid Analytical grade Nanjing Chemical Reagent Company

[0040] Sodium Chloride (AR) Sinopharm Shanghai Chemical Reagent Co., Ltd.

[0041] Liquid milk Mengniu pure milk

[0042] Phthalate Standards Institute of Standards, State Environmental Protection Administration

[0043] double distilled water homemade

[0044] 1.2 Main instruments

[0045] Accela HPLC (including autosampler and accela pump) and TSQ Quantum Access

[0046] Spectrometer Finnigan TSQ Quantum Thermo Scientific

[0047] Vortex Mixer Shanghai Huxi Instrument Co., Ltd.

[0048] RJ-LD-IIB low-speed centrifuge Wuxi Ruijiang Analytical Instrument Co., Ltd.

[0049] Balance AB135-S Shanghai Mettler-Toledo Co., Ltd.

[0050] BP211D electronic analytical balance Germany Sartorius company

[0051] KQ-250DE Ultrasonic Cleaner Ku...

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Abstract

The invention provides a determination method by liquid chromatography-electrospray mass spectrometry (LC-ESI-MS / MS) for detecting various phthalate esters in milk simultaneously, belonging to the field of food detection technology. The milk sample is directly extracted by acetonitrile and sodium chloride; a formic acid ammonium acetate solution-carbinol system is taken as a flowing phase for liquid chromatography separation so as to carry out gradient elution; and the quantitative detection is carried out by a mass spectrum detector by adopting the peak area method of an external standard method. The method has high sensitiveness, good exactness and preciseness, reliable specialization and authenticity, is extremely simple and convenient and easy to be applied to practical detection, and can meet the requirement in practical detection excellently.

Description

technical field [0001] A liquid chromatography-mass spectrometry (LC-ESI-MS / MS) determination method for simultaneous detection of multiple phthalates in milk, specifically a LC-ESI-MS / MS method that can simultaneously quantitatively determine nine phthalates in milk The MS / MS method belongs to the technical field of food detection. Background technique [0002] Phthalatic acid esters (PAEs), mostly colorless oily viscous liquids, easily soluble in fat and organic solvents, insoluble in water, not volatile, contain weak estrogen components, affect biological endocrine , causing mutations, teratogenicity and cancer cell proliferation, is a class of environmental hormones. It can enter the human body through breathing, eating and skin contact, and cause harm to human health. PAEs not only have general toxicity, but also specific toxicity, such as reproductive toxicity, embryotoxicity and genotoxicity. PAEs are the main plasticizers and softeners in the plastics industry. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72
Inventor 胥传来李灼坤丁涛徐丽广
Owner JIANGNAN UNIV
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