The invention provides a miticide for killing plant mites, which is prepared by taking squalene as a main material and adding a solvent, a solubilizer and / or an emulsifying agent, and is a harmonious pesticide which is safe for human and livestock, is environment-friendly and has deep profound significance for long-term development of ecological environment, the human health and comprehensive treatment of diseases. Compared with the traditional pesticide, the plant miticide of squalene has the following characteristics and advantages that: 1, the squalene is abundant in nature, the content ishigher especially in plants and animals, the toxicity thereof is low, the squalene is easily degradable and is safe for the ecological environment; 2, the extracting method of the squalene is simpler, and the squalene can be extracted by techniques of supercritical CO2 extraction and the like; 3, the squalene also can be synthesized artificially, has low cost and is easily used for industrial large-scale production; and 4, due to physical and chemical properties, the squalene can be dissolved in a plurality of solvents, is easily mixed with other mite-killing components for preparing pesticide, and can play a role in improving mite-killing effect.