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36 results about "Recombinant Hirudin" patented technology
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Application of leech to improvement of black eyes or eye puffiness, composition and preparation method of composition
ActiveCN102188360ASolve the real problemImprove or eliminateCosmetic preparationsMake-upMedicineAdditive ingredient
The invention belongs to the field of cosmetics and relates to novel application of leech, particularly to application of the leech to improvement of black eyes or eye puffiness, a composition prepared by using the leech as the main raw material and a preparation method of the composition. For making better use of the novel application of the leech, the invention provides the composition which is prepared by using the leech as the main raw material and is used for improving or removing the black eyes or the eye puffiness. The main active ingredient of the composition is any one of leech extract, natural hirudin and recombinant hirudin; and one or more of pearl, cassia seed extract and Indian buead extract can be selectively added into the composition to form a compound composition, wherein the weight ratios of all the ingredients of the compound composition are as follows: 5-25 parts of leech extract, natural hirudin or recombinant hirudin, 0-5 parts of pearl, 0-5 parts of cassia seed extract, 0-5 parts of Indian buead extract, and at least one of the pearl, the cassia seed extract and the Indian buead extract is not equal to zero. In the invention, the leech is utilized to improve or remove the black eyes and the eye puffiness, the effect is obvious and a novel selection is provided for the public.
Owner:CHONGQING DUOPUTAI PHARMA
Construction of recombinant eukaryotic hansenula engineering bacterial strains containing medical hirudin genes and production process of recombinant hirudin
ActiveCN102373216AHigh expressionImprove efficiencyFungiMicroorganism based processesProtein targetBacterial strain
The invention discloses construction of recombinant eukaryotic hansenula engineering bacterial strains containing medical hirudin genes and a production process of recombinant hirudin, wherein hansenula polytype host cell strains RB-11 are adopted as initial bacterial strains for constructing hansenula high-expression engineering bacterial strains RBHV 145-6 containing hirudin, and the hirudin with high purity and high content is obtained through culture and fermentation. In the invention, the hirudin genes are copied onto host cell chromosomes in a multi-copy mode, in addition, the copying number is kept stable, exogenous gene expression boxes containing the hirudin genes are completely integrated onto the host cell chromosomes, the existence of free plasmids in seed bank cells and passage 40 and 80 generation cells is avoided, the expression quantity and the secretion efficiency of foreign protein are 10 times higher than those of saccharomyces cerevisiae, particularly through beingcompared with that of the polytypic hansenula in China, the expression quantity of the foreign protein is high and is 35.8 percent, and in addition, the excessive glycosylation phenomenon does not exist, so the production process is suitable for target protein through large-scale fermentation production.
Owner:元昊 +1
Preparation method of recombinant hirudin
PendingCN110684101AIncrease productivityHigh expressionMicroorganism based processesFermentationBiotechnologyEngineering
The invention relates to the technical field of fermentation, particularly provides a preparation method of recombinant hirudin. On one hand, the invention provides the preparation method of the recombinant hirudin. The preparation method comprises the steps as follows: placing a seed solution in an initial culture medium, when the DO value is larger than 55%, dropwise adding a supplement culturemedium to the initial culture medium, wherein the preparation process of the initial culture medium comprises the steps as follows: sterilizing a sugar solution and adding to sugar solution to a fermentation tank; performing filtration sterilization on a trace metal solution, and adding the trace metal solution to the fermentation tank; and performing filtration sterilization on a vitamin solutionand adding the vitamin solution to the fermentation tank to obtain the initial culture medium.
Owner:上海松皓生物科技有限公司
Use of leech for cleaning skin, skin cleaning composition and preparation method thereof
The invention, belonging to the field of cosmetics, relates to a new use of leech, specifically to the use of leech as main raw material for cleaning skin, a skin cleaning composition prepared based on the characteristic and a preparation method. According to the invention, microcirculation of skin is improved by means of the blood circulation-promoting and stasis-dissolving effect of leech extract, natural hirudin and recombinant hirudin in order to relax skin as well as thoroughly cleanse dirt, blackheads and whiteheads in pores and aged cuticles (commonly referred to as dead skin) on the surface of skin and the like, therefore, superior foundation is laid for basic skincare. Based upon the above new use, the leech extract, the natural hirudin and the recombinant hirudin can be prepared to cosmetic cleansing products, including face cleansing cream, face cleansing gel, scrubbing cream, cream for removing dead skin, body wash, makeup remover, perfumed soap, etc.
Owner:CHONGQING DUOPUTAI PHARMA
Recombinant bacterium for producing sulfo-recombinant hirudin as well as preparation method and application of recombinant bacterium
ActiveCN113699086ARealize the sulfolation reactionHigh activityBacteriaAntibody mimetics/scaffoldsEscherichia coliEnzyme Gene
The invention discloses a recombinant bacterium for producing sulfo-recombinant hirudin as well as a preparation method and application of the recombinant bacterium, and belongs to the technical field of genetic engineering. In order to improve the synthesis yield of the sulfated recombinant hirudin and reduce the production cost, the invention provides the recombinant bacterium for producing the sulfo-recombinant hirudin, wherein the recombinant bacterium overexpresses a sulfotransferase gene and a hirudin gene, and an original strain is escherichia coli; and the nucleotide sequence of the hirudin gene is one of SEQ ID No.1 to SEQ ID No.3, and the nucleotide sequence of the sulfotransferase gene is as shown in SEQ ID No.4. According to the method, the activity of the recombinant hirudin is remarkably improved, a one-step purification method of the hirudin is realized, a recombinant hirudin product with relatively high purity can be obtained at one time, and the pressure of subsequent purification is greatly reduced.
Owner:QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
Traditional Chinese medicinecomposition containing recombinant hirudin suitable for eyes and preparation method
PendingCN110639010AImprove and relieve visual impairmentImprove and relieve eye fatigue symptomsSenses disorderPeptide/protein ingredientsBlurred visionIrritation
The invention relates to the technical field of eye treatment, in particular to a traditional Chinese medicine composition containing recombinant hirudin suitable for eyes, preparations, preparation method and application. The traditional Chinese medicine composition containing the recombinant hirudin suitable for the eyes is prepared from the components in percentage by weight: 1-3% of oil phasecomponents,0.3-0.8% of a solubilizer, 5-15% of recombinant hirudin fermentation broth, 0.8-2% of traditional medicine components and the balance of residual water phase components. The traditional Chinese medicine composition can relieve and treat eye fatigue and dry eye diseases, can improve and alleviate visual impairment, symptoms of the eye fatigue with dry sensation, foreign body sensation, cautery sensation, itchy sensation, low light, eye redness, blurred vision, visual fluctuation and eye pain irritation are improved. In addition, eye skin black circles, fish tail lines, pouch, swelling and other symptoms are further alleviated and eliminated.
Owner:云山化妆品研究开发(广州)有限公司
Leech colon targeted oral preparation and preparation method thereof
ActiveCN102362874AExcellent releaseExcellent bioavailabilityPeptide/protein ingredientsPill deliveryCoating systemBULK ACTIVE INGREDIENT
The invention relates to the field of preparation, in particular to a leech colon targeted oral preparation prepared by taking leech extract, natural hirudin and recombinant hirudin as active ingredients and a preparation method thereof. The invention aims at solving the technical problems that a leech colon targeted oral preparation which is released and absorbed and gives curative effect to be played at a colon part is provided and bioavailability of oral administration is improved. The preparation method comprises the following steps of: preparing the active ingredients into a coating front midbody, then applying a colon targeted coating system onto the coating front midbody, wherein the coating system comprises an inner coating layer and an outer coating layer; the inner coating layer is mixture of 1 part of guar gum, 1-3 parts of sodium alginate and 1-3 parts of inulin, the mixture is dissolved with water and is prepared into coating solution with the concentration of 2-5%, and weight of the coating is increased by 3-9%; and the outer coating layer is mixture of a film forming agent and a porogenic agent, the outer coating layer is in a weight proportion relation that 6-8 parts of the film forming agent and 2-4 parts of the porogenic agent are adopted, and the weight of the coating is increased by 3-15%. The oral preparation provided by the invention can be released and absorbed at the colon part, and the bioavailability can be improved.
Owner:CHONGQING DUOPUTAI PHARMA
Repairing eye cream
InactiveCN108703927AGood storage stabilityImprove repair effectCosmetic preparationsToilet preparationsEye lotionDimethyl siloxane
Owner:宁波博睿修存生物科技有限公司
Leech tyrosine sulfonate transferase gene and application thereof
ActiveCN113789335AHigh anticoagulant activityStrong substrate preferenceFungiBacteriaTyrosineAcyl group
The invention discloses a leech tyrosine sulphonate transferase gene and application thereof, and belongs to the field of biomedicine. The leech tyrosine sulphonate transferase gene is characterized in that on the basis of a hirudo nipponia transcriptome database, a leech tyrosine sulphonate transferase cDNA sequence is excavated and identified through a bioinformatics means, and on this basis, the leech tyrosine sulfotransferase is synthesized through codon optimization, gene synthesis and microbial cell expression, and the recombinant hirudin is catalyzed by the leech tyrosine sulfotransferase to be subjected to sulfonation modification to obtain the natural hirudin. The catalytic ability of the transferase is greatly improved compared with that of acyl sulfonate transferase, human-derived tyrosine sulfonate transferase and bovine-derived tyrosine sulfonate transferase. Compared with other tyrosine sulfonic acid transferase, the leech tyrosine sulfonic acid transferase obtained in the invention has stronger substrate preference for recombinant hirudin, is suitable for large-scale preparation of natural hirudin, and provides a new way for synthesis of natural hirudin.
Owner:INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
Tumor-specific recombinant hirudin and preparation method and application thereof
InactiveCN107216385AStrong characteristicGood effectPeptide/protein ingredientsAntineoplastic agentsTumor targetLymphatic Spread
The invention discloses a tumor-specific recombinant hirudin. A tail end N of the recombinant hirudin contains an oligopeptide sequence Pro-Gly-Arg-Val-Val that can be identified and split by uPA, wherein the uPA can be highly expressed by a tumor tissue; and the hirudin for recombination is hirudin isomer, hirudin mutant, hirudin chimera, truncated hirudin peptide, and gene modified hirudin or hirudin fusion protein. Correspondingly, the invention further provides a preparation method of the tumor-specific recombinant hirudin and application thereof in preparation of anti-tumor medicines, and provides a preparation method and application of adoptive immune cells expressing the tumor-specific recombinant hirudin. The hirudin anticoagulant activity of the recombinant hirudin is featured by tumor targeted release, and the recombinant hirudin creates a micro-environment where thrombus is difficult to form, and even dissolves formed small thrombus so as to realize functions of anticoagulation, thrombolysis and prevention of metastasis of the tumor cell, so that the risk of systemic bleeding caused by application of wild hirudin to the whole body is prevented.
Owner:新钥(南京)生物科技有限公司
Use of leech for cleaning skin, skin cleaning composition and preparation method thereof
ActiveCN102178635BObvious effectRemove dirtCosmetic preparationsToilet preparationsCutaneous microcirculationCutin
Owner:CHONGQING DUOPUTAI PHARMA
Construction of recombinant eukaryotic hansenula engineering bacterial strains containing medical hirudin genes and production process of recombinant hirudin
ActiveCN102373216BMaintain specific activityCopy number stabilityMicroorganism based processesFermentationProtein targetBacterial strain
The invention discloses construction of recombinant eukaryotic hansenula engineering bacterial strains containing medical hirudin genes and a production process of recombinant hirudin, wherein hansenula polytype host cell strains RB-11 are adopted as initial bacterial strains for constructing hansenula high-expression engineering bacterial strains RBHV 145-6 containing hirudin, and the hirudin with high purity and high content is obtained through culture and fermentation. In the invention, the hirudin genes are copied onto host cell chromosomes in a multi-copy mode, in addition, the copying number is kept stable, exogenous gene expression boxes containing the hirudin genes are completely integrated onto the host cell chromosomes, the existence of free plasmids in seed bank cells and passage 40 and 80 generation cells is avoided, the expression quantity and the secretion efficiency of foreign protein are 10 times higher than those of saccharomyces cerevisiae, particularly through beingcompared with that of the polytypic hansenula in China, the expression quantity of the foreign protein is high and is 35.8 percent, and in addition, the excessive glycosylation phenomenon does not exist, so the production process is suitable for target protein through large-scale fermentation production.
Owner:元昊 +1
Recombinant hirudin fusion protein with targeting and long-acting functions and coding gene and application thereof
ActiveCN113336860ALong duration of actionReduced risk of systemic bleedingPeptide/protein ingredientsFusions for plasma life prolongingBleeding timeBinding peptide
The invention discloses a recombinant hirudin fusion protein with targeting and long-acting functions and a coding gene and application thereof. The fusion protein is formed by fusing a 'procoagulant platelet' targeting binding peptide, hirudin and an albumin binding domain. The fusion protein has the property of a prodrug, can be quickly activated in vivo and exerts an antithrombotic effect; the effect of the fusion protein is equivalent to that of the hirudin, but the duration of the anticoagulant effect is significantly prolonged compared with that of the hirudin, the bleeding time is significantly reduced compared with that of the hirudin and is close to the level of normal saline, the systemic bleeding risk is very low, and the fusion protein has a broad application prospect.
Owner:PEKING UNIV
Recombinant hirudin eye drop and preparation method thereof
InactiveCN104906037AEasy to prepareGuaranteed storage stabilitySenses disorderPeptide/protein ingredientsOphthalmologyIrritation
The invention discloses a recombinant hirudin eye drop. The recombinant hirudin eye drop takes recombinant hirudin as an effective component and sodium hyaluronate as a thickening agent, and also contains an isoosmotic adjusting agent, a metal complexing agent, a preservative and a pharmaceutically acceptable carrier; and the mass ratio of the sodium hyaluronate to the recombinant hirudin to the isoosmotic adjusting agent to the metal complexing agent to the preservative in the eye drop is 10 to 2 to 15.27 to 50 to 3. The preparation method comprises the following steps: dissolving the sodium hyaluronate, the isoosmotic adjusting agent, the metal complexing agent and the preservative into the pharmaceutically acceptable carrier to prepare a blank matrix of the eye drop, dissolving the recombinant hirudin into the blank matrix of the eye drop, mixing evenly, filtering, degerming and sub-packaging to prepare the recombinant hirudin eye drop. According to the eye drop disclosed by the invention, on one hand, the recombinant hirudin can be retainedin a focal zone for a long period of time; on the other hand, irritation is not generated to sensitive eye tissues; and meanwhile, the storage stability of the recombinant hirudin eye drop can also be ensured.
Owner:广西复鑫益生物科技有限公司
Leech colon targeted oral preparation and preparation method thereof
ActiveCN102362874BRealize mass productionPeptide/protein ingredientsPill deliveryCoating systemBULK ACTIVE INGREDIENT
The invention relates to the field of preparation, in particular to a leech colon targeted oral preparation prepared by taking leech extract, natural hirudin and recombinant hirudin as active ingredients and a preparation method thereof. The invention aims at solving the technical problems that a leech colon targeted oral preparation which is released and absorbed and gives curative effect to be played at a colon part is provided and bioavailability of oral administration is improved. The preparation method comprises the following steps of: preparing the active ingredients into a coating front midbody, then applying a colon targeted coating system onto the coating front midbody, wherein the coating system comprises an inner coating layer and an outer coating layer; the inner coating layeris mixture of 1 part of guar gum, 1-3 parts of sodium alginate and 1-3 parts of inulin, the mixture is dissolved with water and is prepared into coating solution with the concentration of 2-5%, and weight of the coating is increased by 3-9%; and the outer coating layer is mixture of a film forming agent and a porogenic agent, the outer coating layer is in a weight proportion relation that 6-8 parts of the film forming agent and 2-4 parts of the porogenic agent are adopted, and the weight of the coating is increased by 3-15%. The oral preparation provided by the invention can be released and absorbed at the colon part, and the bioavailability can be improved.
Owner:CHONGQING DUOPUTAI PHARMA
Skin scraper with skin care liquid
InactiveCN102846456AConvenient careWon't hurtPeptide/protein ingredientsPharmaceutical containersSide effectMedicine
The invention discloses a skin scraper with skin care liquid. The skin scraper 2 is embedded in a plastic sealing clip 4, the plastic sealing clip is engaged on an engagement groove-equipped liquid storage bottle 8 containing skin care liquid via a silicone rubber sealing gasket 3, the skin care liquid is 1-20ml recombinant hirudin, and the engagement groove-equipped hirudin liquid storage bottle can be replaced. The inventive product adopts skin application to avoid the harm to the human body and at the same time causes no pain. In addition, skin application can maximally reduce side effects of medicine on human bodies, so that the product is safe and reliable. Furthermore, the inventive product can be applied at any time and any place, without requiring cooperation of other family members. The skin scraper with skin care liquid fills in a gap in the rehabilitation process of hemiplegia patients and is good for patients.
Owner:TIANJIN BAILIKANGTAI BIOTECH
Chinese medicinal composition containing recombinant hirudin for treating hyperplasia of mammary glands and preparation method thereof
PendingCN110639011AStable physical and chemical propertiesGood storage stabilityHydroxy compound active ingredientsPeptide/protein ingredientsBiochemistryMammary gland structure
The invention relates to the related technical field of treatment of hyperplasia of mammary glands, in particular to a traditional Chinese medicine composition containing recombinant hirudin for treating hyperplasia of mammary glands and a preparation method thereof. According to a first aspect, the traditional Chinese medicine composition containing the recombinant hirudin for treating the hyperplasia of mammary glands is provided, and is prepared from the components in percentage by weight: 3%-10% of an aqueous phase component and 0.5%-3% of an oil phase component,5%-15% of recombinant hirudin fermentation broth, 0.5%-2% of traditional Chinese medicine components, 0.2%-1% of an assistant and the balance of water. According to the traditional Chinese medicine composition containing the recombinant hirudin for treating the hyperplasia of mammary glands, a technical solution combining the recombinant hirudin fermentation broth and the traditional Chinese medicine components is used, mammary swelling or swelling pain can be relieved or cured in a short time, in addition, the composition has stable physical and chemical properties, the storage stability is good, and the storage time of related products can be better prolonged.
Owner:云山化妆品研究开发(广州)有限公司
Preparation of low-immunogenicity recombinant hirudin mutant
InactiveCN102584988AAvoiding Immunogenicity IssuesImprove the effect of clinical medicationPeptide preparation methodsLeech-based protease inhibitorsEpitopeSite-directed mutagenesis
The invention belongs to the field of biotechnology and particularly relates to a low-immunogenicity recombinant hirudin mutant and a preparation method thereof. The method comprises the following steps of: identifying hirudin dominant antigen epitopes by a phage peptide library and a Pepscan (SPOTS) technology; scanning critical combining residues of dominant antigen epitopes by alanine (ALA) canning technology and then positioning accurately; and removing immunogenicity by using a site-specific mutagenesis technology so as to obtain hirudin mutant, wherein according to obtained hirudin mutant of which the anticoagulant rate activity is not changed basically but the immunogenicity and the immunogenicity are obviously reduced. The low-immunogenicity recombinant hirudin mutant and a preparation method thereof have important signification for overcoming the immunogenicity problem of the conventional hirudin medicaments, improving the clinical treatment effect of hirudin medicaments, and fully exerting clinical using values of the hirudin medicaments.
Owner:CHINA PHARM UNIV
Recombinant hirudin eye drops and preparation method thereof
ActiveCN102727874BEasy to prepareEnsure claritySenses disorderPeptide/protein ingredientsPreservativeCLARITY
The invention provides recombinant hirudin eye drops taking sodium hyaluronate as an auxiliary material and a preparation method thereof. The recombinant hirudin eye drops are technically characterized by containing sodium hyaluronate which has the effects of preventing liquid medicine from overflowing and running off, and enabling the medicine to be retained on lesion for a long time. The invention also discloses a preparation method of the recombinant hirudin eye drops. The method ensures the clarity and stability of the eye drops. In addition to recombinant hirudin and sodium hyaluronate, the recombinant hirudin eye drops prepared by the preparation method also contain an isotonic regulator, a metal complexing agent, a preservative and a pharmaceutically acceptable carrier. The preparation method comprises the following specific steps: dissolving sodium hyaluronate, the isotonic regulator, the metal complexing agent and the preservative in the pharmaceutically acceptable carrier to form blank matrix of eye drops, then dissolving the recombinant hirudin in the blank matrix of eye drops, and preparing the recombinant hirudin eye drops by filtering, sterilizing and sub-packaging. The pH value of the prepared recombinant hirudin eye drops is 4.0-7.5. Experimental results in the pharmacodynamics show that the recombinant hirudin eye drops prepared by the preparation method have the functions of preventing and treating cataract.
Owner:CHINA PHARM UNIV
Method for removing endotoxin in recombinant hirudin protein solution
ActiveCN113072638AHigh recovery rateGood removal effectPeptide preparation methodsAgainst vector-borne diseasesProtein solutionIsocratic elution
The invention relates to the technical field of separation and purification of biological medicines, in particular to a method for removing endotoxin in a recombinant hirudin protein solution. The method performs separation by adopting anion exchange chromatography and comprises the following steps: injection: the recombinant hirudin protein solution is taken for injection, the injection volume is 25%-40% of the maximum load of a chromatographic column, and washing is performed with an equilibrium solution to perform column equilibrium; elution: isocratic elution is performed with an eluent, and an outflow part is collected to obtain a recombinant hirudin eluent; and column regeneration: isocratic elution is performed with regenerated liquid, and an outflow part is collected to obtain an endotoxin eluent. According to the method, according to the strength of adsorption of recombinant hirudin and endotoxin with an anion exchanger under neutral conditions, the isocratic elution is performed by increasing salt concentration in a mobile phase, anda good removal effect is achieved by adjusting the injection volume, thus guaranteeing a relatively high protein recovery rate, and meanwhile, regeneration of the chromatographic column is implemented. The method is simple, efficient, low in cost and suitable for industrial production.
Owner:宁波博睿瀚达生物科技有限公司
Traditional Chinese medicinal external composition containing recombinant hirudin for treating chloasma and preparation method of traditional Chinese medicinal external composition
InactiveCN110743005AStable physical and chemical propertiesGood storage stabilityPeptide/protein ingredientsPharmaceutical delivery mechanismAdjuvantArginine
The present invention relates to the related field of treating melisma and more specifically provides a traditional Chinese medicinal external composition containing recombinant hirudin for treating melasma and a preparation method of the traditional Chinese medicinal external composition. The provided traditional Chinese medicinal external composition containing the recombinant hirudin for treating the melisma comprises the following preparation raw materials in weight percentages: 3-15% of an aqueous phase component, 0.5-3% of an oil phase component, 3-12% of recombinant hirudin fermentationliquid, 0.5-2% of traditional Chinese medicine components, 0.1-1% of an adjuvant and the balance water; wherein the aqueous phase component comprises arginine. Compared with the prior art, the provided traditional Chinese medicinal external composition for treating the melasma is prepared by a method for combining the recombinant hirudin fermentation liquid and the traditional Chinese medicine components, has a better therapeutic effect for different age groups, also has stable physical and chemical properties and relatively good storage stability, and is relatively well prolonged in storagetime.
Owner:上海松皓生物科技有限公司
Recombinant hirudin microneedle drug delivery system and preparation method thereof
PendingCN112237574AImprove complianceRepair barrier functionPeptide/protein ingredientsMacromolecular non-active ingredientsCombinatorial chemistryDrug activity
The invention belongs to the technical field of biological medicines, and relates to a recombinant hirudin microneedle drug delivery system and a preparation method thereof. The recombinant hirudin microneedle drug delivery system comprises a backing patch and a microneedle body located at the bottom of the backing patch, wherein recombinant hirudin is packaged in the microneedle body, and a microneedle matrix is a water-soluble biodegradable high polymer material. Compared with an existing drug delivery mode, the microneedle drug delivery system has the advantages that pain caused by repeatedinjection can be remarkably relieved during use, and the drug is stable in activity and convenient to store and transport.
Owner:FUDAN UNIV
Tumor-specific recombinant hirudin, preparation method and application thereof
InactiveCN107098964AStrong characteristicGood effectPeptide/protein ingredientsAntineoplastic agentsTumor targetLymphatic Spread
The invention discloses tumor-specific recombinant hirudin, of which an N-terminal contains an oligopeptide sequence PLGI, which can be recognized and cleaved by matrix metalloproteinase 9 (MMP9), wherein hirudin used for recombination may be a hirudin isomer, a hirudin mutant, truncated hirudin peptide, gene-modified hirudin or hirudin fusion protein. Correspondingly, the invention provides a preparation method of the tumor-specific recombinant hirudin and applications thereof in preparation of antitumor medicines, and also provides a preparation method and an application of adoptive immune-cells for expression of the tumor-specific recombinant hirudin. The recombinant hirudin herein allows anticoagulation activity of the hirudin is released in a tumor targeted manner, thus forming a microenvironment which is not liable to form thrombus, and even dissolving formed small thrombus to achieve the functions of anticoagulation, thrombolysis and prevention of tumor cell metastasis, thereby overcoming the risk of systematic hemorrhage due to systemic administration of wild-type hirudin.
Owner:新钥(南京)生物科技有限公司
Application of leech to improvement of black eyes or eye puffiness, composition and preparation method of composition
ActiveCN102188360BObvious effectCosmetic preparationsMake-upPuffiness around the eyesAdditive ingredient
Owner:CHONGQING DUOPUTAI PHARMA
Recombinant hirudin liquid storing bottle with ball and spring retainer
InactiveCN102885693AEasy to carryEasy to useDispensing apparatusPharmaceutical containersEngineeringBottle cap
The invention discloses a recombinant hirudin liquid storing bottle with a ball and a spring retainer. The liquid storing bottle comprises a bottle cap and a liquid storing bottle body, wherein a bottle cap extending part is connected below the bottle cap; an inwardly-concave bottle cap lock catch is formed close to a lower end on the inner side of the bottle cap, and is connected to a bump formed on the outer side of the bottle cap extending part; the bottle cap extending part is in threaded connection with the liquid storing bottle body through a liquid storing bottleneck; recombinant hirudin is filled into the liquid storing bottle body; a ball and a ball spring retainer are arranged in the bottle cap extending part; a liquid outlet cavity with a certain gap is formed between the ball spring support and the bottle cap extending part; and the lower end of the ball spring support is connected to the liquid storing bottleneck, and is supported by the surrounding walls of the liquid storing bottleneck. Through skin administration, the side effects of medicaments on human bodies can be reduced to the maximum extent, and a product is safe and reliable. Moreover, the recombinant hirudin liquid storing bottle can be used at any time and at any place, does not require cooperation of family members, and is good news for a large quantity of patients.
Owner:TIANJIN BAILIKANGTAI BIOTECH
Application of hirudin for repairing skin red blood filament or hormone face, composition and preparation method
InactiveCN109260046AInhibition of secretionInhibited DiffusionCosmetic preparationsToilet preparationsInflammatory factorsMedicine
The invention provides an application of hirudin for repairing skin red blood filament or hormone face, a composition and a preparation method thereof, natural hirudin or recombinant hirudin are takenas main active ingredients, and one or two of rhubarb extract and allantoin are taken as active ingredients together for repairing skin red blood filament or hormone face problems. The invention hasthe beneficial effects that hirudin is the main active ingredient, emodin and allantoin are combined to neutralize inflammatory factors, inhibit the secretion and diffusion of inflammatory factors, rebuild skin barrier, and improve the problems of red blood filament and hormonal face.
Owner:重庆雅素生物科技有限公司
New use of recombinant hirudin
InactiveCN1242811CPostpone timeReduce turbiditySenses disorderPeptide/protein ingredientsVeterinary medicineRecombinant Hirudin
The present invention discloses a new application of recombinant hirudin for preparing medicine for preventing and curing catarct.
Owner:CHINA PHARM UNIV
Method for obtaining salivary gland stem cell mass of hirudo nipponia through enzymolysis and application of salivary gland stem cell mass
PendingCN114657120AImprove efficiencyHigh activityCell dissociation methodsEpidermal cells/skin cellsSalivary gland cellGenetic engineering
The invention discloses a method for obtaining salivary gland stem cell clusters of Hirudo nipponia through enzymolysis and application, and relates to the technical field of cell separation.According to the technical scheme, the method is characterized in that the salivary gland tissue blocks of Hirudo nipponia are subjected to multiple times of enzymolysis, and finally the stem cell clusters with the capacity of being split and differentiated into new salivary gland cells are obtained; according to the method, in-vitro long-term culture of salivary gland cells of Hirudo nipponia is realized, the efficiency is higher than that of primary culture for passage of salivary gland cells of Hirudo nipponia obtained by direct separation, natural hirudin is extracted by separating salivary gland cell metabolites, and compared with recombinant hirudin obtained by genetic engineering means, the method has the advantages that the yield is high, and the cost is low. The hirudin obtained by the method is higher in activity and higher in efficiency.
Owner:CHINA JILIANG UNIV
Aroma spray and preparation method thereof
ActiveCN109010248AWith tightnessWith slipperyCosmetic preparationsToilet preparationsStyraxInflammation
The invention relates to cosmetics and in particular relates to aroma spray and a preparation method thereof. The aroma spray provided by the invention is prepared from the following raw materials according to the percentage: 0.2 to 0.8 percent of recombinant hirudin, 0.5 to 1.5 percent of a lactobacillus fermentation product, 5 to 15 percent of fructus kigeliae Aafricanae fruit, 1 to 3 percent ofthyme, 1 to 3 percent of styrax, 3 to 10 percent of a spice, 2 to 6 percent of a humectant, 2 to 8 percent of an organic elastic body and the balance of de-ionized water. The aroma spray provided bythe invention has the characteristics of tightness, smoothness, pore shrinkage, inflammation diminishing and sterilization, unique aroma and the like.
Owner:广州市尚昇生物科技有限公司
A kind of leech tyrosine sulfonate transferase gene and its application
ActiveCN113789335BHigh anticoagulant activityStrong substrate preferenceFungiBacteriaTyrosineAcyl group
Owner:INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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