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Hirudin extracting method

An extraction method, hirudin technology, applied in chemical instruments and methods, from leech inhibitors, protease inhibitors, etc., can solve the problems of low content of active ingredients, many impurities, heavy color, etc., and achieve the effect of vigorous vitality

Inactive Publication Date: 2016-06-22
徐顺青 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The applicant finds that the existing living body extraction method for hirudin has the following defects: first, the existing living body extraction method has a low extraction amount of hirudin
Again, the powdered hirudin obtained by the existing extraction method has a high bacterial content, which can only be used as an oral preparation due to the high bacterial content
Finally, the powdery hirudin obtained by the existing extraction method is black, dark brown or reddish brown, the color is heavy, there are many impurities and the content of active ingredients is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1~6 is the specific implementation of the extraction method of a kind of hirudin of the present invention and preparation method thereof of the present invention, and wherein embodiment 1 is best embodiment. The leeches used in Examples 1 to 2 are Hirudo phenanthrata, the leeches used in Examples 3 to 4 are Japanese medical leeches, and the leeches used in Examples 5 to 6 are brown leeches. The vitamin C aqueous solution in Examples 1-3 is formed by mixing vitamin C and water at a mass ratio of 5:1000, and the vitamin C aqueous solution in Examples 4-6 is formed by mixing vitamin C and water at a mass ratio of 3:1000. In Examples 1 to 6: in step 2), the ultraviolet light source is located 0.8 to 1.5 meters above the leech, the ultraviolet light source is located above the spraying port of the inducer, and the ratio of the power of the ultraviolet light source to the mass of the leech is 0.5 to 2W: 100 ~250 g, the nozzle of the inducer is located below the UV ...

Embodiment 2

[0037] 1) Starvation treatment: keep the ambient temperature at 25-28°C, put the live leeches in vitamin C water solution and let them stand for 7 hours to perform starvation treatment;

[0038] 2) Stimulate secretion: keep the ambient temperature at 25-28°C, and use an air-compressed atomizer to atomize the inducer into tiny droplets under ultraviolet radiation with a wavelength of 250nm-270nm, and spray the atomized inducer on the body of the leech. The table shows that the ultraviolet irradiation time is 60-120 seconds, turn off the ultraviolet rays, and irradiate the leeches for 9-10 minutes in a warm white light off and flickering mode to stimulate the leeches to secrete secretions; repeat the above operation 3 times;

[0039] The color temperature of warm white light is 2700~3200K, the wavelength of green light is 520nm~540nm, and the flicker frequency of warm white light and green light are both 3Hz; the inducer is obtained by diluting chicken blood, cow blood and water ...

Embodiment 3

[0043] 1) Starvation treatment: keep the ambient temperature at 28-32°C, put the live leeches in vitamin C water solution and let them stand for 8 hours to perform starvation treatment;

[0044] 2) Stimulate secretion: keep the ambient temperature at 28-32°C, and use an air-compressed atomizer to atomize the inducer into tiny droplets under ultraviolet radiation with a wavelength of 250nm-270nm, and spray the atomized inducer on the body of the leech. Table, the ultraviolet irradiation time is 60-120 seconds, turn off the ultraviolet light, and irradiate the leech with warm white light and green light alternately flickering for 8-9 minutes to stimulate the leech to secrete secretions; repeat the above operation 3 times;

[0045] The color temperature of warm white light is 2700~3200K, the wavelength of green light is 520nm~540nm, the flicker frequency of warm white light and green light are both 8Hz; the inducer is obtained by diluting chicken blood and water at a volume ratio ...

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Abstract

A hirudin extracting method belongs to the technical field of hirudin extraction and comprises the following steps: 1), starving: 2), stimulating for excretion: holding ambient temperature to 23-40 DEG C, spraying an attractant to the body surface of a leech under ultraviolet irradiation at 200 nm and 275 nm in wavelength for 30-180 seconds, turning off ultraviolet, irradiating the leech for 3-15 min by using a warm white light in a flashing manner, or irradiating the leech for 3-15 min by using a warm white light and a green light in an alternating flashing manner such that the leech is stimulated to secrete a secretion; 3), sterilizing; 4), concentrating. The hirudin extracting method is high in hirudin extraction quantity and allows repeated extraction, the powdered hirudin finally obtained has light color and high level of active ingredients.

Description

technical field [0001] The invention relates to a method for extracting hirudin, which belongs to the technical field of hirudin extraction. Background technique [0002] Hirudin is the most active and most researched ingredient among the various active ingredients extracted from leeches and their salivary glands. It is a small molecular protein (single-chain polypeptide) composed of 65-66 amino acids. Hirudin has a strong inhibitory effect on thrombin, and is the strongest natural specific inhibitor of thrombin found so far. It has a high curative effect on human cardiovascular and cerebrovascular diseases, especially thrombotic diseases such as cerebral thrombosis. It is incomparable to any substance (including aspirin and heparin, which are currently routinely used in clinical practice), because: on the one hand, the antithrombotic function of natural hirudin does not require the participation of other factors, and its molecular weight is much smaller than that of heparin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/815C07K1/14
CPCC07K14/815
Inventor 徐顺青常传云常越徐茂林
Owner 徐顺青
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