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Bionic nano fiber material as well as preparation method thereof and application thereof

A fiber material and biomimetic nanotechnology, applied in the field of medical biomaterials, can solve problems such as weak mechanical force and chemical toxicity

Active Publication Date: 2020-10-20
ZHEJIANG MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For natural biological materials that are not easily plasticized, freeze-drying and chemical cross-linking are mostly used, but there are defects of weak mechanical force and chemical toxicity

Method used

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  • Bionic nano fiber material as well as preparation method thereof and application thereof
  • Bionic nano fiber material as well as preparation method thereof and application thereof
  • Bionic nano fiber material as well as preparation method thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0043] The preparation method of the biomimetic nanofiber material provided in this embodiment includes: compounding the silk fibroin-chitosan nanofiber membrane and the liquid crystal collagen, so that a part of the liquid crystal collagen is filled into the silk fibroin-chitosan nanofiber membrane In the fiber pores, another part of the liquid crystal collagen covers the surface of the silk fibroin-chitosan nanofiber membrane.

[0044] Concrete preparation steps are:

[0045] S1. Preparation of silk fibroin-chitosan nanofibers by electrospinning.

[0046] (1) Prepare a mixed electrospinning solution of silk fibroin and chitosan.

[0047] The mixture of chitosan and silk fibroin with a mass ratio of 0.23-0.25:1 is dissolved in a formic acid solution with a mass concentration of 80-98%, and stirred by magnetic force for 3-5 hours to obtain a uniformly dispersed mixed electrospinning solution. Preferably, the mass fraction of the obtained electrospinning solution is preferabl...

Embodiment 1

[0082] The preparation method of the biomimetic nanofiber material provided in this embodiment is specifically:

[0083] First prepare a high-concentration collagen solution:

[0084] The rat tail tendon was extracted, dissolved in 0.1% acetic acid solution for 48h, centrifuged at 4000rpm, 4°C for 30min, the supernatant was taken, and dialyzed for 7d. The dialysate was frozen overnight at -80°C, placed in a freeze dryer, and freeze-dried for 72 hours to obtain freeze-dried collagen. Dissolve freeze-dried collagen in 0.5mol / L acetic acid solution to prepare a 120mg / mL solution, stir magnetically at low temperature until dissolved, and use low-temperature interval ultrasonic method to disperse the collagen evenly. The collagen is dispersed evenly and then the spinning solution is prepared:

[0085] Extract regenerated silk fibroin: wash the cocoons and dissolve them in 0.5% Na 2 CO 3 Boil in aqueous solution to remove allergen sericin, dry and dissolve in CaCl 2 / C 2 h 5 ...

Embodiment 2

[0093] The preparation method of the biomimetic nanofiber material provided in this embodiment is specifically:

[0094] First prepare a high-concentration collagen solution:

[0095] The tail tendons of rats were extracted, dissolved in 0.1% acetic acid solution for 50 hours, centrifuged at 3800 rpm, 3°C for 25 minutes, and the supernatant was dialyzed for 7 days. The dialysate was frozen overnight at -85°C, placed in a freeze dryer, and freeze-dried for 76 hours to obtain freeze-dried collagen. Dissolve the freeze-dried collagen in 0.4mol / L acetic acid solution to prepare a 100mg / mL solution, stir magnetically at low temperature until dissolved, and use low-temperature interval ultrasonic method to disperse the collagen evenly. Collagen is dispersed evenly.

[0096] Next prepare the spinning solution:

[0097] Extract regenerated silk fibroin: wash the cocoons and dissolve them in 0.5% Na 2 CO 3 Boil in aqueous solution to remove allergen sericin, dry and dissolve in Ca...

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Abstract

The invention discloses a bionic nano fiber material as well as a preparation method thereof and application thereof, and relates to the technical field of medical biological materials. The bionic nano fiber material comprises a silk fibroin-chitosan nano fiber film and liquid crystal state collagen, wherein one part of liquid crystal state collagen is filled into holes of the nano fiber film, andthe other part of the liquid crystal state collagen is coated to the surface of the nano fiber film. The preparation method comprises the following steps: compounding the silk fibroin-chitosan nano fiber film with a high-concentration collagen solution to obtain a mixture; balancing the mixture in acetic acid solution steam, and self-assembling the collagen into the liquid crystal state collagen.The liquid crystal state collagen can be used as cell activating mesenchyme of the silk fibroin-chitosan nano fiber film, can adhere a great number of cells, can trend a cell growth direction, and also can form long-bundle fibers, so that mechanical properties of the nano fiber film are improved. The bionic nano fiber material has good mechanical property, relatively strong cell adhesion and no immunogenicity, is beneficial for cell repair, and can be applied to dressing, scaffolds for tissue engineering and drug delivery bodies.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to a bionic nanofiber material and its preparation method and application. Background technique [0002] With the development of biotechnology, biomaterials are prepared into biomimetic medical materials such as dressings, stents, and drug delivery bodies, which have been widely used in the repair and replacement of damaged or necrotic tissues and organs. Ideal biomimetic medical materials should have good cell compatibility, degradability, plasticity and certain mechanical force. Biomimetic materials are often synthetic polymer materials and natural biological materials; although synthetic polymer materials have strong plasticity and mechanical force, they degrade slowly and the degradation products are acidic, which can easily cause non-specific inflammatory reactions in surrounding tissues and immune response. Natural biomaterials, such as collagen, chitosan, hyalu...

Claims

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Application Information

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IPC IPC(8): D04H1/728D04H1/4382A61L15/28A61L15/32A61L15/42A61L15/40A61K47/36A61K47/42C12N15/87C12N5/00
CPCA61K47/36A61K47/42A61L15/28A61L15/32A61L15/325A61L15/40A61L15/42A61L15/425C12N5/0075C12N15/87C12N2533/50C12N2533/72D04H1/4382D04H1/728C08L5/08C08L89/00
Inventor 花扣珍银国利徐建军巫甜甜刘丹丹王俊娟冀磊
Owner ZHEJIANG MEDICAL COLLEGE
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