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Exosome preparation for promoting skin wound healing and preparation method thereof

A technology of skin wounds and exosomes, applied in the field of stem cells, can solve the problems of slow skin healing process, affect the appearance, and easy to leave scars, achieve fibroblast proliferation and repair, improve the surrounding vascular microenvironment, promote skin damage repair, The effect of moisturizing wound repair effect

Active Publication Date: 2020-09-08
广州赛琅生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The healing process of this kind of wound skin is slow. If it is not handled properly, it is easy to cause infection, and it is easy to form scars, which affects the appearance and brings great troubles to people.
There are currently some products for treating burns, scalds, trauma, etc., most of which only play an analgesic and anti-inflammatory effect. Skin injuries heal slowly, and scars are easy to leave in the later stage

Method used

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  • Exosome preparation for promoting skin wound healing and preparation method thereof
  • Exosome preparation for promoting skin wound healing and preparation method thereof
  • Exosome preparation for promoting skin wound healing and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] An exosome preparation for promoting skin wound healing, including the following raw materials in parts by mass: 30 parts of stem cell exosome extract, 10 parts of Zhanba flower extract, 10 parts of witch hazel extract, 10 parts of chamomile extract, 0.2 part of allantoin, 0.05 part of alginate, 5 parts of PEG-40 hydrogenated castor oil, 0.05 part of hyaluronic acid, 4 parts of butanediol, 0.1 part of methylparaben, 0.1 part of hydroxyethyl cellulose, EDTA- 0.1 part of disodium and 33 parts of deionized water.

[0039] The preparation method of the above stem cell exosome extract comprises the following steps:

[0040] (1) Carry out cell culture in accordance with GMP, use cell factory equipment to expand mesenchymal stem cells, and harvest 3×10 cells when the cell fusion rate reaches more than 95%. 8 mesenchymal stem cells;

[0041] (2) Ultrasonic disrupt the mesenchymal stem cells obtained in step (1), press 1×10 8 Add the number of cells to 100ml of normal saline,...

Embodiment 2

[0056] An exosome preparation for promoting skin wound healing, including the following raw materials in parts by mass: 30 parts of stem cell exosome extract, 20 parts of Zhanba flower extract, 20 parts of witch hazel extract, 20 parts of chamomile extract, 0.3 part of allantoin, 0.08 part of alginate, 2 parts of PEG-40 hydrogenated castor oil, 0.08 part of hyaluronic acid, 1 part of butylene glycol, 0.1 part of methylparaben, 0.5 part of hydroxyethyl cellulose, EDTA- 0.05 parts of disodium and 4 parts of deionized water.

[0057] According to the preparation method in Example 1, the stem cell exosome extract, chamba flower extract, witch hazel extract and chamomile extract were prepared.

[0058] The preparation method of the above-mentioned exosome preparation for promoting skin wound healing comprises the following steps:

[0059] S1 Take allantoin, alginate, hyaluronic acid, EDTA-disodium in proportion, dissolve in deionized water, stir and mix evenly;

[0060] S2 Add bu...

Embodiment 3

[0064] An exosome preparation for promoting skin wound healing, comprising the following raw materials in parts by mass: 50 parts of stem cell exosome extract, 10 parts of Zhanba flower extract, 10 parts of witch hazel extract, 10 parts of chamomile extract, 1 part of allantoin, 1 part of alginate, 1 part of PEG-40 hydrogenated castor oil, 0.1 part of hyaluronic acid, 5 parts of butanediol, 0.3 part of methylparaben, 1 part of hydroxyethyl cellulose, EDTA- Disodium 0.1 part and deionized water 11 parts.

[0065] According to the preparation method in Example 1, the stem cell exosome extract, chamba flower extract, witch hazel extract and chamomile extract were prepared.

[0066] The preparation method of the above-mentioned exosome preparation for promoting skin wound healing comprises the following steps:

[0067] S1 Take allantoin, alginate, hyaluronic acid, EDTA-disodium in proportion, dissolve in deionized water, stir and mix evenly;

[0068] S2 Add butanediol, methylpar...

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Abstract

The invention relates to an exosome preparation for promoting skin wound healing and a preparation method thereof, and belongs to the technical field of stem cells. The exosome preparation for promoting skin wound healing is prepared from the following components in parts by mass: 30 to 50 parts of stem cell exosome extract, 10 to 20 parts of plumeria rubra extracting solution, 10 to 20 parts of witch hazel extract and 10 to 20 parts of chamomile extract. The preparation method is simple, and all the raw materials are uniformly mixed in parts by mass. By adding stem cell exosome and the components such as plumeria rubra, chamomile, witch hazel and the alginate, wound hemostasis is accelerated, and injured tissue repair and skin regeneration are promoted; and meanwhile, the effects of resisting inflammation, inhibiting bacteria, relieving itching and moisturizing are achieved. The product has the characteristics of good effect, quick effect, convenient use, no irritation to skin mucosa,safety, reliability and no scar.

Description

technical field [0001] The invention belongs to the technical field of stem cells, and in particular relates to an exosome preparation for promoting skin wound healing and a preparation method thereof. Background technique [0002] Exosomes are cystic vesicles produced by cells through a series of biological mechanisms such as endocytosis-fusion-efflux and excreted from the outside of cells through active secretion. Their essence is a lipid bilayer. Exosomes contain miRNA and various molecular proteins, which participate in the regulation of various physiological functions of cells. Exosomes can play a role in the treatment of various diseases by virtue of the therapeutic efficacy of street stem cells with biologically active substances such as proteins and miRNAs carried. Studies have shown that exosomes can inhibit inflammation, promote skin cell proliferation and angiogenesis, reduce the accumulation of myofibroblasts, and repair damage through paracrine methods, providi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P17/02A61K35/28
CPCA61K35/28A61K36/185A61K36/24A61K36/28A61P17/02A61K2300/00
Inventor 冯焯威陈镇洲
Owner 广州赛琅生物技术有限公司
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