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Multilayer film and cell culture container

a cell culture container and multi-layer technology, applied in the field of cell culture technology, can solve the problems of inability to achieve a high degree of cell density, and insufficient gas permeability of a conventional culture bag, so as to improve cell proliferation efficiency and improve gas permeability of the cell culture container

Inactive Publication Date: 2013-06-27
TOYO SEIKAN GRP HLDG LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention improves the gas permeability of a cell culture container without affecting its handling properties. This means that higher density films can be used for the container, allowing for better cell proliferation efficiency.

Problems solved by technology

A dish or a flask which is commonly used for cell culture is not suited to the culture of a large amount of cells.
Gas permeability of a conventional culture bag was, however, not sufficient.
As compared with an open culture container such as a dish, a conventional culture bag cannot attain a high degree of cell density.

Method used

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  • Multilayer film and cell culture container
  • Multilayer film and cell culture container
  • Multilayer film and cell culture container

Examples

Experimental program
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first embodiment

[0027]The multilayer film and the cell culture container according to the first embodiment of the present invention will be explained with reference to FIG. 1 and FIG. 2. FIG. 1 is a view showing the configuration of the multilayer film of this embodiment, and FIG. 2 is a view showing the configuration of a cell culture container formed by the multilayer film of this embodiment.

[0028]As shown in FIG. 1, the multilayer film of this embodiment is used for forming a cell culture container, and has a base material 1 forming the main body part of the cell culture container and an inner layer 2 (innermost layer) forming the cell culture surface.

[0029]The base material 1 and the inner layer 2 are formed of a material having a high gas permeability, heat sealing properties and transparency. The inner layer 2 is formed of a material having a low cytotoxicity in addition to the above-mentioned properties. As examples of such a material, a polyethylene-based resin such as linear low density po...

second embodiment

[0047]Then, the multilayer film and the cell culture container according to the second embodiment of the present invention will be explained with reference to the drawings. FIG. 3 is a view showing the configuration of the multilayer film of this embodiment, and FIG. 4 is a view showing the configuration of the cell culture container formed by the multilayer film of this embodiment.

[0048]In this embodiment, the multilayer film has a configuration in which a polyethylene-based resin having a density of 0.886 g / cm3 to 0.93 g / cm3 is used as an outer layer 3 in the first embodiment. In the multilayer film of this embodiment, one or two or more outer layers may be provided between the base material 1 and the outer layer 3. Further, between the base material 1 and the inner layer 2, one or two or more inner layers may be provided.

[0049]If the density of the outer layer 3 is smaller than 0.886 g / cm3, production and handling of a cell culture container using the multilayer film may become m...

example 1

[0058]A cell culture film formed of three layers was subjected to co-extrusion molding by the T-die method by using a labo plastomill (manufactured by Toyo Seiki Co., Ltd.). At this time, as the base material, Kernel KS240T (LLDPE, manufactured by Japan Polyethylene Corporation, density, 0.880 g / cm3) was used. As the inner layer and the outer layer, a resin obtained by blending Excellen GMHCB 2001 (LDPE, manufactured by Sumitomo Chemical Corporation, density, 0.920 g / cm3) and Kernel KM 262 (LLDPE, manufactured by Japan Polyethylene Corporation, density, 0.898 g / cm3) at an amount ratio of 6:4 was used. In the cell culture film thus produced, the thickness of the base material was 66 μm, the thickness of the inner layer was 19 μm, the thickness of the outer layer was 12 μm, and the total thickness of the cell culture film was 97 μm.

[0059]By using this cell culture film, the oxygen permeability per unit thickness (ml·mm / m2·day·atm (37° C.-80% RH)) was measured by means of a gas permeab...

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Abstract

Provided are a multilayer film having excellent gas permeability and excellent handling properties and hence is suited for forming a cell culture container, and a cell culture container formed by using the same.A multilayer film used for forming a cell culture container, comprising: a base material composed of a polyethylene-based resin having a density of 0.87 g / cm3 to 0.90 g / cm3; and an inner layer composed of a polyethylene-based resin having a density of 0.896 g / cm3 to 0.93 g / cm3 and forming a cell culture surface. A cell culture container is formed by using this multilayer film.

Description

TECHNICAL FIELD[0001]The present invention relates to a cell culture technology, in particular, relates to a multilayer film having excellent gas permeability and a cell culture container.BACKGROUND ART[0002]In recent years, in the field of production of medical supplies, gene therapy, regenerative therapy, immune cell therapy or the like, a large amount of a cell, a tissue, a microorganism or the like are required to be cultured effectively in an artificial environment. A dish or a flask which is commonly used for cell culture is not suited to the culture of a large amount of cells. Under such circumstances, a culture bag is used as a culture container for the culture of a large amount of cells. A culture bag is preferable not only since it enables a large amount of cells to be cultured but also it can reduce the risk of contamination by a fungi or virus due to its closed system.[0003]As the conditions required for a resin film constituting a culture bag, gas permeability, transpar...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12M1/04B32B1/00B32B7/02
CPCC12M23/20Y10T428/24992Y10T428/2495C12M23/24B32B27/32C12M23/14B32B2250/02B32B2250/03B32B2250/242B32B2307/72B32B2323/046B32B7/02B32B27/08B32B1/08B32B2307/31B32B2307/412B32B2307/724B32B2307/732B32B2439/40B32B1/00C12M3/00C12M1/16B32B27/00
Inventor TANAKA, SATOSHITOTANI, TAKAHIKOISHIZAKI, YOICHIOTA, KYOHEISUENAGA, RYOKUNINORI, MASAHIRO
Owner TOYO SEIKAN GRP HLDG LTD
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