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36 results about "Sterol carrier protein" patented technology

Sterol carrier proteins (also known as nonspecific lipid transfer proteins) is a family of proteins that transfer steroids and probably also phospholipids and gangliosides between cellular membranes. These proteins are different from plant nonspecific lipid transfer proteins but structurally similar to small proteins of unknown function from Thermus thermophilus.

Sterol Carrier Protein-2 Inhibitors for Lowering Cholesterol and Triglyceride Levels in Mammals

InactiveUS20080194658A1Lowering serum total cholesterol concentrationLowering serum low density lipoprotein cholesterol concentrationBiocideOrganic active ingredientsHydrobromideSerum ige
Methods of treating high serum levels of total cholesterol, low density lipoprotein and triglycerides in a mammal by administering therapeutically effective doses of N-(4-{[4-(3,4-dichlorophenyl)-1,3-thiazol-2-yl]amino}phenyl)acetamide or a salt thereof such as a hydrochloride or hydrobromide salt.
Owner:ZAMA JAPAN

Monoclonal antibody of hepatitis B virus X protein and use thereof

The invention relates to a monoclonal antibody of hepatitis B virus X protein (HBx), which is characterized by having specificity reaction to N-end epitope or C-end epitope of HBx, but having no reaction to keyhole limpet hemocyanin (KLH) of carrier protein and other expressed proteins of hepatitis B virus (HBV). The preparation method of the monoclonal antibody comprises the steps of: fusing N-end and C-end antigen epitope polypeptides of HBx respectively with mice immunized with KLH in crosslinking way, and myeloma cells and screening to obtain the monoclonal antibody. The monoclonal antibody can be used for various immunodetection reagents and directed therapeutic drugs of HBx protein or HBx antibody and applied to diagnosis and treatment of diseases such as hepatitis b virus (HBV) infection, hepatocellular carcinoma (HCC) and the like.
Owner:王虹

Escherichia coli t7 expression vector, vectors for the co-expression and co-purification of recombinant peptides in/with carrier proteins, use of expression vectors for obtaining complexes with multiple antigens and immonomodulators

The present invention relates to a vector for the expression of recombinant proteins, antigens, pathogen-like particles and immunogenic complexes, said vector (pMRKA vector) being produced by modifying the plasmids containing the gene sequence of the T7 promoter of E. coli, this modification being mainly characterized by the substitution of the ampicillin-resistance gene by the kanamycin-resistance gene, and by the insertion of the par sequence (partition sequence which determines the efficient segregation of the plasmids in daughter cells during cell division). Also provided are expression vectors based on the pMRKA plasmid, which additionally comprise at least one of the gene sequences of the exosome of P. abyssi, which vectors are designated pMRKA-EXO, pMRKA-RING and pSUMAC. The invention also provides the vectors additionally comprising gene sequences with immunomodulatory or immunoregulatory activity, preferably the pMRKA-Z-Z-EXO and pMRKA-Z-Z-RING vectors. Other aspects of the invention include the method for producing said expression vectors and the use of the obtained vectors.
Owner:OURO FINO SAUDE ANIMAL

Carrier protein of bacterial polysaccharide conjugate vaccine and application thereof

The invention discloses a bacterial polysaccharide O-glycosylation modified recombinant cholera toxin B subunit fusion protein and an application thereof. The present invention provides a conjugate of polysaccharide and protein which is coupled by the bacterial polysaccharide and recombinant cholera toxin B subunit fusion protein. The bacterial polysaccharide is connected with the glycosylation sites of the recombinant cholera toxin B subunit fusion protein in the form of O-glucosidic bond. The experiment indicates that the preparation of the bacterial polysaccharide protein conjugate vaccine from O-glycosylation modified recombinant cholera toxin B subunit fusion protein can enhance the ability of inducing animals to generate anti-polysaccharide antibodies, avoid miscellaneous problems of pathogen culture, enhance the vaccine homogeneity and production efficiency, and reduce the vaccine preparation cost, therefore possessing wide application prospect.
Owner:INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE

Preparations and uses of streptomycin-carrier protein coupled product and streptomycin antibody

The invention discloses a coupling product of streptomycin and carrier protein, and a preparation method of a streptomycin antibody and an application thereof, which relate to a coupling method of the carrier protein such as keyhole limpet hemocyanin, human serum albumin, cow serum albumin, ovalbumin and the like with streptomycin, and immune BALB / C mouse spleen cells are fused with SP2 / 0 mouse myeloma cells by streptomycin immunogens coupled with the carrier protein, and the streptomycin coupled with the carrier protein is used as a coating antigen for screening positive hybridoma and hybridoma that can steady passage and excrete anti-specificity streptomycin monoclonal antibody can be obtained by the cell clone, and ascites monoclonal antibody is prepared. The prepared monoclonal antibody is used for building a direct competition ELISA method with high specificity, sensitivity and accuracy to the streptomycin and immunity colloidal gold test strips. The coupling of the streptomycin and the carrier protein and the preparation method of the streptomycin monoclonal antibody can provide services for detecting streptomycin residue in foods quickly.
Owner:ZHEJIANG UNIV

Synthetic method of urethane artificial antigen

The invention provides a synthetic method of urethane artificial antigen. The urethane artificial antigen is prepared by the following steps of: 1, preparation of beta-alanine and ethyl chloroformate; 2, synthesis of hapten; 3, purification of hapten; 4, preparation of 1-(3-dimethylamino propyl)-3-ethyl carbodiimide solution; 5, synthesis of artificial antigen; 6, dialysis and detection of artificial antigen; and 7, calculation of quantity of different used carrier proteins. According to the synthetic method, the shielding effect of the protein to the hapten obtained by chemical synthesis is low, therefore, high-titer polyclonal antibody can be generated in an immune animal, and the molecular weight can be detected during synthesizing the artificial antigen, and the coupling ratio can be calculated, and the quantity of used raw materials for synthesizing is precise.
Owner:ZHEJIANG FORESTRY UNIVERSITY

Carrier protein, recombinant expression vector, exosome and preparation method and application of exosome

The invention provides carrier protein, recombinant expression vector, an exosome and a preparation method and application of the exosome. Foreign protein can be carried into the exosome efficiently through fusion of the carrier protein with the foreign protein. Through the recombinant expression vector, a to-be-expressed foreign protein peptide fragment gene sequence is inserted into polyclonal sites of the recombinant expression vector, so that efficient and directed expression of the foreign protein in the cell exosome is achieved; meanwhile, the invention also provides the preparation method of the exosome for carrying the foreign protein, foreign gene can be brought into the exosome effectively, and simple and easy operation, a stable process and good applicability are achieved; and the exosome prepared by using the preparation method contains the foreign protein, has good cell permeability and highly efficiency of cell transfection, the activity and efficacy of the foreign protein can be developed at the same time, and a broad application prospect is achieved.
Owner:JINAN UNIVERSITY

Bacterial polysaccharide protein conjugate vaccine using hepatitis B surface antigen as carrier protein and preparation method of bacterial polysaccharide protein conjugate vaccine

InactiveCN104383532AAddressing Immunization IssuesPlay a role in preventionAntibacterial agentsAntiviralsAntigenConjugate vaccine
The invention discloses a bacterial polysaccharide protein conjugate vaccine using a hepatitis B surface antigen as carrier protein and a preparation method of the bacterial polysaccharide protein conjugate vaccine. According to the vaccine, protein is the hepatitis B surface antigen, and a bacterial polysaccharide is selected from any one or more of a haemophilus influenza type b polysaccharide, group A, group C, group Y and group W135 meningococcal polysaccharides, a salmonella typhi type Vi polysaccharide, a group B streptococcus type Ia polysaccharide and the like, pneumococcus serotype type 1, 2 and the like, and salmonella paratyphi type A or salmonella paratyphi type B. Animal experiments show that the antibody positive conversion rates of the bacterial polysaccharide and the hepatitis B surface antigen in the vaccine are both more than 85%, so that the vaccine is relatively high in antibody positive conversion rate; carrier protein plays a role in transforming the bacterial polysaccharide from a T-cell-independent antigen into a T-cell-dependent antigen, and also can be used for preventing diseases caused by hepatitis B virus; and by adopting the bacterial polysaccharide protein conjugate vaccine disclosed by the invention, the problem of performing immunization inoculation on infants and young children under 2 years old can be solved, the function of one injection with multiple immune effects also can be achieved, and the use crowd and coverage rate of the vaccine can be expanded.
Owner:云南沃森生物技术股份有限公司

Conjugate, and preparation and use thereof

The invention discloses a coupling object. The coupling object is obtained through the coupling of at least one protein in the four proteins of a recombined human epidermal growth factor and a recombined epidermal growth factor mutant with a carrier protein. The amino acid sequence of the recombined human epidermal growth factor is shown by the No.2 sequence in a sequence list. The recombined epidermal growth factor mutant can be one of the following proteins: 1)the protein obtained through the deletion of the No.53 arginine in the No.2 sequence in the sequence list; 2) the protein obtained through the deletion of the No.53 arginine and No.52 leucine in the No.2 sequence in the sequence list; and 3) the protein obtained through the addition of methionine before the No.1 asparagine in the No.2 sequence in the sequence list. The coupling object can be used as a vaccine for the active treatment of an epithelium sourced tumor after being mixed with an adjuvant. Results show that the vaccine can induct the high level antibodies in a mouse; the highest antibody titer reaches 1:20,000; and the prolonging rate of the survival period of the mouse reaches 69.4 percent.
Owner:BIOTECH PHARMA CO LTD +3

Glycosylated polypeptide, conjugate of glycosylated polypeptide and carrier protein, as well as preparation method and application of glycosylated polypeptide

The invention provides glycosylated polypeptide, a conjugate of the glycosylated polypeptide and carrier protein, as well as a preparation method and application of the glycosylated polypeptide. The glycosylated polypeptide has a sequential structure of Glyco-Val-His-Leu-Thr-Pro-Tyr-Ryr-Cys; the conjugate of the glycosylated polypeptide and the carrier protein is a product generated after the tail end Crs of the glycosylated polypeptide is coupled with the carrier protein (such as bovine serum albumin, myohemoglobin or hemocyanin); the glycosylated polypeptide or the conjugate of the glycosylated polypeptide and the carrier protein can be used for replacing an expensive pure human glycosylated hemoglobin antigen product to prepare a chromatographic testing bar (testing paper bar); the chromatographic testing b bar can be used for rapidly, accurately, quantitatively, simply and conveniently detecting the glycosylated hemoglobin in the whole blood.
Owner:BEIJING YICHENG BIOELECTRONICS TECHNOLOGY COMPANY

Preservation method of cotton bollworm sterol carrier protein-2

ActiveCN107177619AMeet demandRapid cessation of metabolic activityBacteriaMicroorganism based processesCotton bollwormDissolution
The invention relates to the field of biotechnology and in particular to a preservation method of cotton bollworm sterol carrier protein-2. The preservation method of the cotton bollworm sterol carrier protein-2 designed by the invention comprises the following steps: (1) constructing genetic engineering bacteria; (2) abundantly expressing target protein; (3) digging 10-20g thallus with a medicine spoon each time, placing the thallus in liquid nitrogen, freezing the thallus into solid spherical bacterium balls under the action of rapidly cooling the liquid nitrogen, and after the obtained thallus is frozen into a plurality of bacterium balls by the liquid nitrogen, placing all the bacterium balls in a refrigerator at the temperature of 80 DEG C below zero for preservation; and (4) according to the required protein quantity, taking a proper amount of the bacterium balls out of refrigerator with the temperature of 80 DEG C below zero per time, dissolving the bacterium balls into a buffer solution, enabling the bacterium balls to be sequentially subjected to cell disruption and protein purification after complete dissolution, and obtaining the target protein. The preservation method can ensure the content and the activity of the protein used in each test study to keep relatively consistent and also saves tedious procedure required for each induction culture.
Owner:HUBEI UNIV OF TECH

Cotton bollworm sterol carrier protein 2 inhibitor and virtual screening method thereof

The invention belongs to the technical field of agricultural biotechnologies, and relates to establishment of a cotton bollworm sterol carrier protein 2 (SCP-2) small-molecule inhibitor virtual screening method. The method comprises the following steps: according to structural data of cotton bollworm sterol carrier protein 2 (SCP-2), analyzing a key amino acid residue so as to confirm a binding pocket; screening, scoring and calculating binding free energy by using molecular docking software; performing structure clustering and visual analysis on an obtained compound, thereby obtaining a small-molecule inhibitor for SCP-2 protein. The small-molecule inhibitor can be applied to screening, study and development of novel environmental-friendly pesticides for multiple agricultural pests of lepidoptera as the main target.
Owner:HUBEI UNIV OF TECH

Allogenic animal serum albumin as carrier protein for preparation of semiantigen-antibody

The present invention relates to a preparation method for a semi-antigen antibody. In details, the present invention discloses a preparation method for semi-antigen antibody by means of serum albumin of various animals as the carriers. According to the preparation method, first of all, semi-antigens of serum albumin of various animals are coupled into full antigens; and then the full antigens are used to generate antibodies. The serum albumin is protein which exists in the animal body since the embryo development. The immune cells already have immune no-response and the serum albumin is of no allotype or co-phenotype allele. The serum albumin does not vary from animal to animal and takes a large amount in the blood. Serum albumin can be easily obtained and the purifying process is simple. Therefore, the semi-antigen antibody prepared through the method is of higher value and better specificity.
Owner:SHANGHAI KEXIN BIOTECH

Cell strain with sterol carrier protein (SCP) stable transformation of prodenia litura and preparation method and application of cell strain

The invention discloses a preparation method of a cell strain with sterol carrier protein (SCP) stable transformation of prodenia litura. The preparation method sequentially includes the steps of 1), cloning cDNA (complementary deoxyribonucleic acid) of SCP (single-cell protein)-2 gene from prodenia litura; 2), cloning the cDNA of the SCP-2 encoded protein into pcDNA5 / FRT plasmid to form recombined pcDNA5 / FRT-SCP2 plasmid; 3), applying the pcDNA5 / FRT-SCP2 plasmid to transform colon bacillus and obtaining transformed colon bacillus via cultivation; 4), extracting recombined plasmid DNA from the transformed colon bacillus and performing purification; 5), transfecting Chinese hamster ovary cells with purified and recombined pcDNA5 / FRT-SCP2 plasmid DNA; 6), selecting transfected CHO cells in a culture medium containing antibiotic hygromycinB and performing continuous subculture to obtain the cell strain. The invention aims to provide the cell strain with SCP stable transformation of the prodenia liture, the preparation method of the cell strain and an application of the cell strain. The cell strain has the advantages of high screening accuracy, simpleness in operation and accuracy in result.
Owner:SOUTH CHINA NORMAL UNIVERSITY

Preparation for self-protein used as carrier protein for hapten-antibody

The present invention relates to hapten antibody preparing process, and is especially the process of first preparing complete antigen through coupling animal's autologous protein to hapten and subsequent preparing antibody through immunizing animal with the complete antigen. The antibody preparing process has no introduced foreign protein, body produced antibody targeting to the small molecular weight compound and small peptide hapten, and no antibody to autologous protein. Therefore, the prepared hapten antibody prepared with autologous protein as carrier protein has high valence and high specificity.
Owner:SHANGHAI KEXIN BIOTECH

Fusion protein for treating intestinal diseases

ActiveUS20190241639A1Weaken the chain exchange phenomenon characteristic of the IgG4 isotypeExtended half-lifePeptide/protein ingredientsAntibody mimetics/scaffoldsIntestinal structureDisease
The invention provides fusion proteins for treating an intestinal disease, having a structure as follows: R-L-P, wherein R is a GLP-2 receptor agonist; L is a peptide linker; and P is a long-acting carrier protein. The fusion protein provided by the invention has significant bioactivities and in vitro stability.
Owner:ZHEJIANG DOER BIOLOGICS CO LTD

Immunogenic composition

The invention provides an immunogenic composition comprising: a) a conjugate that is a capsular saccharide from GBS serotype Ia conjugated to a carrier protein; b) a conjugate that is a capsular saccharide from GBS serotype Ib conjugated to a carrier protein; c) a conjugate that is a capsular saccharide from GBS serotype III conjugated to a carrier protein; d) a conjugate that is a capsular saccharide from GBS serotype II conjugated to a carrier protein; and e) a conjugate that is a capsular saccharide from GBS serotype V conjugated to a carrier protein.
Owner:GLAXOSMITHKLINE BIOLOGICALS SA

A vaccine composition and uses thereof

The present invention provides a vaccine composition comprising: (i) an adjuvant; and (ii) at least one fusion peptide conjugated to a carrier protein, wherein the carrier protein is the diphtheria toxin variant CRM-197 (GenBank Accession No. and wherein the at least one fusion peptide comprises two or more non- contiguous B cell epitopes of Her2 / neu selected from the group consisting of SEQ ID Nos:1-7 and 15-60 and amino acid sequences that have at least 85% identity to any of the foregoing. The present invention also extends to pharmaceutical compositions and methods of using the vaccine and / or pharmaceutical compositions, as herein described, for the treatment or prevention of a cancer characterised by the expression or over-expression of Her2 / neu in a patient in need thereof.
Owner:BIOLIFE SCI QLD

Monoclonal antibody for hippuric acid antigen

InactiveUS20090227777A1Higher competitive inhibitionEngineer specificityImmunoglobulins against animals/humansRecombinant DNA-technologyMetaboliteProtein L
Provided is a monoclonal antibody specific for hippuric acid which is one of the representative harmful hallucinogenic substances and is the major metabolite of toluene. In the present invention, a hippuric acid-carrier protein conjugate is prepared from hippuric acid and BSA or OVA as a carrier protein, using a coupling reagent and a cross-linker, mice are immunized by injection of the resulting conjugate, splenocytes are collected from animals and fused with myeloma cells, fused cells are cultured in HAT medium, a cell line producing a hippuric acid-directed monoclonal antibody is screened using a detection conjugate, a gene coding for variable regions of the screened monoclonal antibody is amplified by RT-PCR and then amplified by SOE-PCR using a linker DNA to thereby prepare a single chain variable fragment (scFV) which is cloned into a vector, thereby sequencing the base sequence and deducing amino acid sequences of the variable regions. The monoclonal antibody screened according to the present invention has a titer having a standard curve in the concentration range of mg/mL meeting requirements for the permissible exposure limit (PEL) of toluene, exhibits no cross-reactivity with carrier proteins, exhibits higher competitive inhibition in response to an increasing concentration of hippuric acid and exhibits no cross-reactivity with other proteins contained in the urine, and therefore can be usefully employed in a diagnostic kit for detection of hippuric acid which is capable of diagnosing toluene exposure.
Owner:IND ACADEMIC CORP FOUND YONSEI UNIV +1

Preservation method of sterol carrier protein 2 of cotton bollworm

ActiveCN107177619BMeet demandRapid cessation of metabolic activityBacteriaMicroorganism based processesBiotechnologyCotton bollworm
The invention relates to the field of biotechnology and in particular to a preservation method of cotton bollworm sterol carrier protein-2. The preservation method of the cotton bollworm sterol carrier protein-2 designed by the invention comprises the following steps: (1) constructing genetic engineering bacteria; (2) abundantly expressing target protein; (3) digging 10-20g thallus with a medicine spoon each time, placing the thallus in liquid nitrogen, freezing the thallus into solid spherical bacterium balls under the action of rapidly cooling the liquid nitrogen, and after the obtained thallus is frozen into a plurality of bacterium balls by the liquid nitrogen, placing all the bacterium balls in a refrigerator at the temperature of 80 DEG C below zero for preservation; and (4) according to the required protein quantity, taking a proper amount of the bacterium balls out of refrigerator with the temperature of 80 DEG C below zero per time, dissolving the bacterium balls into a buffer solution, enabling the bacterium balls to be sequentially subjected to cell disruption and protein purification after complete dissolution, and obtaining the target protein. The preservation method can ensure the content and the activity of the protein used in each test study to keep relatively consistent and also saves tedious procedure required for each induction culture.
Owner:HUBEI UNIV OF TECH

Verticillium dahliae adp-atp carrier protein disease course key target gene and its interference carrier and application

The invention discloses a verticillium dahliae ADP-ATP carrier protein (AACP) pathogenesis key target gene as well as an interference vector and application thereof, belonging to the fields of cloning and application of a verticillium dahliae pathogenesis key gene. According to the invention, 3 sections of pathogenesis key target genes which are capable of significantly reducing disease index in a plant are screened from an AACP gene by virtue of a host induced gene silencing technology, and the 3 sections of target segments are further used for constructing a Gateway interference vector, so as to obtain transgenic tobacco which is significantly high in pathogenic bacterium resistance and stable in genetic performance. Through disease index and fungal biomass analysis as well as transcriptional level detection of the target gene, an optimum interference section of the AACP gene is finally screened and obtained. The verticillium dahliae ADP-ATP carrier protein pathogenesis key target gene and the RNA interference vector disclosed by the invention are applicable to improving disease resistance of plants to verticillium dahliae and breeding new varieties of transgenic plants resisting verticillium dahliae.
Owner:THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI

Cotton Sterol Carrier Protein Gene and Its Application

A sterol carrier protein gene of cotton and applications thereof are disclosed. The sterol carrier protein gene is Gh SCP2D. The ORF sequence of the gene is shown as SEQ ID NO.1. The genomic sequence of the gene is shown as SEQ ID NO.2. Upland cotton is subjected to transgenic research by constructing a plant antisense expression vector, and expression analysis shows that expression of the gene is obviously inhibited, a phenotype of reduced velvet length is shown in transgenic plant, and the sucrose content is obviously reduced, so that the sterol carrier protein gene Gh SCP2D can be applied in sucrose transportation modification and cotton fiber elongation modification in cotton fiber development processes.
Owner:NANJING AGRICULTURAL UNIVERSITY
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