Sinkiang drug mulberry tissue culture and rapid propagation culture medium

Abstract

A set of Sinkiang drug mulberry tissue culture and rapid propagation culture medium comprises the following components: a germination culture medium, a multiplication culture medium, an extension culture medium and a root culture medium, wherein the germination culture medium is prepared from the following raw materials: MS 4.33 g / L, 6-BA 2.0 mg / L, NAA 0.2 mg / L, sugar 30 g / L and agar 7 g / L, and the pH value of the germination culture medium is 5.8; the multiplication culture medium is prepared from the following raw materials: MS 4.33 g / L, 6-BA 1.0 mg / L, sugar 30 g / L and agar 7 g / L, and the pH value of the multiplication culture medium is 5.8; the extension culture medium is prepared from the following raw materials: MS 4.33 g / L, ZT 4.5 micro-mol / L, sugar 30 g / L and agar 7 g / L, and the pH value of the extension culture medium is 5.8; and the root culture medium is prepared from the following raw materials: WPS 2.30 g / L, L+NAA 0.2 mg / L, IBA 0.2 mg / L, activated carbon 0.4 g / L, sugar 20 g / L and agar 6 g / L, and the pH value of the root culture medium is 6.0. The culture medium is simple and convenient to prepare, and is high in multiplication efficiency, each seedling can differentiate 2.3 lateral buds averagely, the transplanted seedlings are got by winter buds after four months, and the survival rate after transplantation is high to more than 75%.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a set of Xinjiang medicinal mulberry tissue culture rapid propagation medium. Background technique [0002] Xinjiang medicine mulberry belongs to Moraceae, Morus, and black mulberry species (Morus nigra Linn.) in plant taxonomy. It was introduced into my country from western Iran in the 16th century and is mainly cultivated in Hotan, Kashgar, Aksu and other vast areas in southern Xinjiang. It is an important medicinal mulberry germplasm resources. Xinjiang medicine mulberry is a deciduous tree with a height of 3-7m or higher and a larger crown. The germination period is mid-to-late April, and the leaf-opening period is early May; the ripening period of mulberries is from mid-to-late June to mid-to-late August, and the fruit shedding period is in late August. After ripening, the fruit is purple-black or purple-red, sour Sweet and delicious, with a high juice yield. ...

AI Technical Summary

Problems solved by technology

However, the compatibility between Xinjiang medicine mulberry and the diploid mulberry tree used as rootstock is poor, and it is not easy to survive after grafting

However, there is no research report on the successful and rapid propagation of Xinjiang medicine mulberry tissue culture

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