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Novel paecilomyces lilacinus strain, as well as preparation method and application of same

A technology of Paecilomyces lilacinus and a new strain, which is applied in the field of microbial preparation and soil fertilizer, can solve the problem of not being able to improve the performance of killing nematode eggs, and achieve good ability to kill nematode eggs, high efficiency, and long storage period Effect

Pending Publication Date: 2017-12-19
山东福沃嘉生物肥料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation method of the patent application for Paecilomyces lilacinus fungicides is common and common Paecilomyces lilacinus, which cannot improve its nematocidal egg performance

Method used

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  • Novel paecilomyces lilacinus strain, as well as preparation method and application of same

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preparation example Construction

[0027] like figure 1 As shown, according to a non-limiting embodiment of the present invention, a preparation method of a bacterial agent containing a new strain of Paecilomyces lilacinus is provided, comprising the following steps.

[0028]Step S1: Inoculate the Paecilomyces lilacinus species in the lyophilized tube into the PDA medium and culture them for 5 days to obtain healthy Paecilomyces lilacinus colonies on a sterile operating bench, and set the culture temperature at about 28 degrees Celsius.

[0029] Step S2: Inoculate healthy colonies of Paecilomyces lilacinus into nutrient agar medium and culture them at about 28 degrees Celsius for 5 days.

[0030] Step S3: Select healthy bacterial strains for future use.

[0031] Step S4: Insert the spare healthy strains into the sterilized liquid medium and cultivate them for 4 days to obtain Paecilomyces lilacinus seed liquid. The speed of the liquid medium is set at 150 rpm and the temperature is about 38-45°C. Celsius. Pa...

Embodiment 1

[0039] Identification of Paecilomyces lilacinus

[0040] Paecilomyces lilacinus of the present invention has following colony form and form:

[0041] (1) The colony grows fast on the malt juice agar medium (MEA), cultured in the dark at 28°C for 5 days, and the diameter of the colony is 40-50mm; (2) The colony is initially white, and then the colony gradually changes from white to light pink to Light purple, flocculent texture, no water-soluble pigment; (3) observed under the microscope, the conidiophores and branches of Paecilomyces lilacinus are more dispersed than those of Penicillium, and the conidiophores form dry chains towards the base, forming finger-like Branches; (4) Single spores are ovoid to spindle-shaped, colorless, and will grow flat, lavender characteristic colonies on solid plate medium.

[0042] The above characteristics are consistent with the characteristics of Paecilomyces lilacinus.

[0043] The DNA sequence of Paecilomyces lilacinus of the present inve...

Embodiment 2

[0046] Preparation of Paecilomyces lilacinus inoculum

[0047] The present invention adopts a liquid+solid joint fermentation culture process to cultivate Paecilomyces lilacinus, through liquid multiplication culture and then inserting into solid culture multiplication culture, the formed bacteria agent has strong biological enzyme activity. The specific steps include the following steps.

[0048] Seed preparation and liquid multiplication culture: activate the freeze-dried tubes containing Paecilomyces lilacinus, the specific method is to first insert the strains in the freeze-dried tubes into the slope of the PDA medium on the aseptic operating table at 28 Cultivation was carried out for 5 days. After obtaining healthy colonies, insert them into nutrient agar medium and culture them at about 28°C for 5 days, then select and develop healthy strains and insert them into sterilized liquid medium at 150r / min at about 42°C Cultivate for 3 days to obtain Paecilomyces lilacinus s...

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Abstract

The invention discloses a novel paecilomyces lilacinus strain, as well as a preparation method and an application of same. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) at December, 28th, 2016, and is assigned the accession number CGMCC No.13379. The preparation method includes the steps of: preparing a paecilomyces lilacinus seed liquid; inoculating the seed liquid onto a solid proliferation culture medium and performing proliferation culture under a sterile aerobic condition, culture temperature being 25-45 DEG C and culture time being 5-10 days; drying the culture medium after the proliferation culture to prepare a raw bacterial agent of paecilomyces lilacinus. The novel paecilomyces lilacinus strain is used for preventing and treating nematodes on crops and has excellent capability of killing ova of the nematodes; a test proves that activity of chitinase in a fermentation product of the strain is high, so that the novel strain can be used for prevention and treatment on nematodes on roots of crops.

Description

technical field [0001] The invention belongs to the field of microbial preparation and soil fertilizer, in particular to an agricultural microbial preparation and its application. Background technique [0002] Plant parasitic nematodes are an important class of plant pathogens. Among the four major pathogens that cause plant infectious diseases, their harm exceeds that of bacteria and viruses, and is second only to fungal viruses. According to reports, plant parasitic nematodes cause about US$100 billion in global agricultural production losses every year, and root-knot nematodes (Meloidogyne spp.) only cause tens of billions of US dollars in annual losses to important economic crops in the world. Its host range is wide, the damage is serious, and the damaged plants are more susceptible to fungal and bacterial infections, which has become one of the important reasons for inducing other plant diseases. The research history of root-knot nematode egg-parasitic fungi is not lon...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/79
CPCC12N1/14A01N63/30C12R2001/79C12N1/145
Inventor 康洪波孙洪录
Owner 山东福沃嘉生物肥料有限公司
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