38 results about "Chitinase activity" patented technology

The present invention provides isolated nucleic acids encoding polypeptides with chitinase activity and methods for using the nucleic acids to enhance resistance of plants to fungal and nematode infections.

The invention relates to the application of trichoderma strains in preparation of biopesticide for preventing and controlling cucumber fusarium wilt. The trichoderma strains are Trichoderma harzianum SH2303CGMCC NO.4963. The main components for preventing and controlling the cucumber fusarium wilt are conidia of the Trichoderma harzianum SH2303; and the biopesticide can be seed coating agents or granules. The trichoderma strains in the application has high growth speed and high sporulation quantity; the confrontation pathogens inhibition rate in vitro reaches 43.01 percent; the inhibition rate of a non-volatile inhibitor to the cucumber fusarium wilt is 41.33 percent; the inhibition rate of a volatile inhibitor to the cucumber fusarium wilt is 11.49 percent; the chitinase activity is 3.9573U; the beta-1,3-glucanase enzyme activity is 0.5092U; the extracellular protease enzyme activity is 3.0678 U; the prevention effect aiming at the living bodies of the cucumber fusarium wilt is 71.43 percent; the overall proportion of the antibiosis secondary metabolites, having antagonism, in the strains is 30.28 percent; and the trichoderma strains can effectively prevent and treat the disease of the cucumber fusarium wilt.

The present invention is a method for preparing a biocontrol formulation by the following steps. A Streptomyces bacterium is selected simultaneously with superior sporulation ability, chitinase activity and antagonistic effectiveness against plant fungal pathogens by culturing with fungal pathogens, wherein the selected Streptomyces bacterium is cultured on a chitin-limited plate to assess the ability of sporulation and chitinase activity, and the Streptomyces bacterium with superior sporulation ability is the strain with producing powder-like and maturity form spore chains. Spores from the selected bacterium is collected. The obtained spores are taken as a seed inoculum. Then the seed inoculum is cultured to directly yield a broth medium containing at least 109 viable spores / ml which the spores contained in the broth medium are well suspended. The biocontrol formulation is obtained. The present invention further provided a biocontrol formulation containing a high concentration of Streptomyces spp. spores.

Excess sludge production in a bioreactor of a wastewater treatment plant in which excess sludge is being produced is reduced by adding an activated sludge material having a chitinase specific activity of at least 150 Units / g-MLSS and a pectinase specific activity of at least 120 Units / g-MLSS to the bioreactor. After the addition of the activated sludge material, the bioreactor is controlled by adding the activated sludge material when any one of the chitinase activity, the pectinase activity, and the protease specific of the sludge in the bioreactor drops below the lower limits of 50 Units / L, 40 Units / L, and 0.3 Units / L, respectively.

This invention relates to pesticide and antiparasitic compositions for the control of pests, diseases and parasites attacking plants and animals. The compositions include, at least one chitinolytic agent or a chitinolytic activity-inducing agent, and sulfide or a sulfide-producing agent from microorganisms or chemical compounds, wherein the chitinolytic agent or the chitinolytic activity-inducing agent and sulfur or a sulfur-producing agent obtaining from microorganisms or chemical compounds are concurrently applied at a range significantly lower than any of the above-mentioned compounds, when they are individually to attain effective control.

The invention provides a chitinase-producing Bacillus subtilis strain SH21, a method for producing chitinase, microbial preparations and applications. The Bacillus subtilis strain SH21 has chitinase-producing activity. The chitinase activity of the Bacillus subtilis strain after fermentation can reach 4.02U / mL, and after the fermentation product is purified by a combination of salting out, gel filtration chromatography and ion exchange chromatography, the purification factor of chitinase is increased by 7.23 times, the specific activity is 154.5U / mg. The present invention also provides the application of the bacillus subtilis strain or microbial preparation in inhibiting the growth of Fusarium solani.

The invention belongs to the field of microorganisms and biotechnology, and in particular relates to a compound bacterial agent of microorganisms and its application in increasing production and quality of Angelica sinensis against diseases. The compound microbial agent is composed of Pseudomonas fluorescens, Pseudomonas alcaligenes and Pseudomonas psychrophilus; it has ACC deaminase activity, SOD activity, POD activity, NEX neutral xylan Enzyme activity, chitinase activity, and laccase activity; can promote the growth of the roots of the underground medicinal organs of Angelica sinensis, increase the diameter of the reed head, the length of the reed head, the length of the main root, and the average weight per plant, not only increasing the production but also improving the quality of commercial Angelica sinensis. Appearance properties; can increase the content of ferulic acid, Z-ligustilide, ligustilide A, endogenous jasmonic acid, salicylic acid, etc., which can significantly improve the quality of Angelica sinensis, and can be used to prepare special microorganisms for Angelica sinensis Fertilizer; it can prevent and control plant diseases caused by pathogenic fungi of the genus Fusarium, and can be used to prepare pesticides for preventing and controlling plant diseases.

A strain of streptomyces producing chitinase and its application, the invention relates to a strain of streptomyces producing chitinase and its application. The purpose of the present invention is to solve the problem that existing methods for controlling soybean gray mold and black spot have hidden dangers of pesticide residues. A strain of chitinase-producing Streptomyces of the present invention is Streptomyces (Streptomyces sp.) G6 , preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is February 2, 2018, and the preservation number is CGMCC No.15334. The application of the chitinase-producing streptomyces of the present invention refers to the application in the prevention and control of soybean diseases. Strain G6 not only has chitinase activity, but also has good antagonistic effect on soybean gray mold and black spot, has no pesticide residue, and does not produce drug resistance. The invention is applied to the technical field of environmental microorganisms.

The invention discloses a method for fermenting and producing chitinase by Chaetomium globosa and its application. The strain is activated by using Chaetomium globosa HY1, and the activation medium includes glucose, peptone, potassium dihydrogen phosphate, dihydrogen phosphate Sodium; then shaker culture or fermenter culture, the fermentation medium includes soluble starch, glycerol, tryptone, yeast powder, corn steep liquor, NH 4 NO 3 , MnSO 4 , MgSO 4 、Na 2 HPO 4 、K 2 HPO 4 , Colloidal chitin, the chitinase produced by fermentation is used for the preparation of chitooligosaccharides. The cultivation method of the invention is simple, the nutritional requirements are not high, the chitinase produced by liquid fermentation has high activity, and has great industrial application value.

The invention provides a biological compound potash fertilizer and a preparation method of the biological compound potash fertilizer. The biological compound potash fertilizer is prepared by the following steps of: fermenting pathogenic fungus CGMCC (China General Microbiological Culture Collection Center) No.5155 which resists 12.5% of potash and has chitinase activity to obtain a biological potash fertilizer; and mixing 8-15 parts of the biological potash fertilizer, 1-5 parts of dipotassium phosphate and 10-15 parts of humic acid potash fertilizer to prepare the biological compound potash fertilizer. The biological compound potash fertilizer provided by the invention is simple in preparation method, strong in product compositionality and strong in parataxis and has the functions of evenly providing nutrients, improving soil fertility, strengthening the stress resistance of crops, decreasing the crop diseases, improving the quality of agricultural products, decreasing the harmful substances in the agricultural products and the like.

The invention relates to genetically engineered bacillus amyloliquefaciens which can effectively prevent gray mold and its preparation method and application, belonging to the field of molecular biology. In the present invention, through genetic engineering, the chitinase gene ech42 of G. pink is amplified, and the expression vector pHT43 is connected to construct the recombinant expression vector pHT43 / ech42. By setting electric shock transformation, the recombinant expression vector pHT43 / When ech42 is introduced into Bacillus amyloliquefaciens, the electric shock voltage is 12.5kv / cm, 15kv / cm, or 17.5kv / cm. In the detection of chitinase activity of the present invention, the enzyme activity of the pHT43-ech42 / BA strain at the 8th hour is about 54.3% higher than that of the wild-type strain BA. In terms of preventing Botrytis cinerea, the disease prevention effect is still 28.9% after spraying for 20 days. % or so, with long-lasting biological control effect.

The invention relates to a chitinase-producing bacterial strain and its application. Its taxonomic name is Streptomyces amylase ( Streptomyces diastaticus ) CS1801, the deposit number is CCTCC NO: M2018263. Streptomyces amylase CS1801 of the present invention ( Streptomyces diastaticus ) CS1801 is derived from naturally fermented hemp shrimp paste. By fermenting hemp shrimp, the highest enzymatic activity of chitin was 57.3U / L, and the content of chitooligosaccharides was 0.58 mol / L. The invention provides a new method for the production of chitooligosaccharides and has good application prospects.

The invention discloses a African Trichoderma harzianum for disease prevention and growth promotion and application thereof. The strain number of Trichoderma harzianum is TM2-4, and its registration number in the General Microorganism Center of China Committee for Culture Collection of Microorganisms is CGMCC No.15881. Trichoderma harzianum M2‑4 has inhibitory effects on tomato cinerea, peach brown rot, pepper anthracnose and cucumber wilt on fruit and vegetable crops; Germination and plant growth are promoted. Trichoderma harzianum TM2‑4 has indoleacetic acid (IAA)-producing activity, siderophilic activity, glucanase-producing activity and chitinase-producing activity. Trichoderma harzianum TM2‑4 has a fast propagation speed, can be artificially cultured, and the culture conditions are simple. The highest spore production in the bran corncob nutrient solution solid-state fermentation medium can reach 3.2×10 9 cfu / g solid bacterial agent.