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A kind of Pseudomonas compound microbial bacterial agent and its application in improving yield and quality of Angelica sinensis

A technology of composite microbial agent and Pseudomonas, applied in the direction of microorganism-based methods, microorganisms, biocides, etc., can solve the problems of not being able to improve at the same time, and improving disease resistance without angelica growth, so as to achieve the goal of improving resistance Effect

Active Publication Date: 2022-03-08
GANSU ACAD OF SCI INST OF BIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And existing literature reports, the content of plant endogenous salicylic acid and jasmonic acid is negatively correlated, that is, can not improve salicylic acid and jasmonic acid in Angelica sinensis simultaneously.
At present, there is still no microbial agent that can simultaneously promote the growth of Angelica sinensis, improve disease resistance, improve the appearance quality of Angelica sinensis, and the content of various iconic components

Method used

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  • A kind of Pseudomonas compound microbial bacterial agent and its application in improving yield and quality of Angelica sinensis
  • A kind of Pseudomonas compound microbial bacterial agent and its application in improving yield and quality of Angelica sinensis
  • A kind of Pseudomonas compound microbial bacterial agent and its application in improving yield and quality of Angelica sinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Composite microbial bacterial agent related enzyme activity detection

[0038] Inoculate the above-mentioned bacteria into 20mL PDB medium respectively, shake culture (30°C, 180r / min) for 24 hours; collect the bacteria by centrifugation at 5000r / min, resuspend the bacteria with sterilized distilled water until the turbidity of the bacteria solution is about 1 , add 1mL of the corresponding enzyme extract in the kit to a 1.5mL centrifuge tube, and add 100μL of the bacteria solution to be tested, break up the bacteria by ultrasonication in an ice bath (power 200W), ultrasonication for 3s, with an interval of 10s, and repeat 30 times; the ultrasonicated bacteria Centrifuge the solution for 10 min (4°C, 9000r / min), draw the supernatant into a new centrifuge tube, and put it on ice for testing; measure the enzyme activity according to the method described in the corresponding assay kit, and divide the calculated results by the corresponding Turbidity of bacterial s...

Embodiment 2

[0042] Example 2 Effects of Different Inoculum Treatment on Physiological and Biochemical Characteristics of Angelica Sinensis Leaf

[0043] According to the method described in the kit, the chlorophyll, MDA, H 2 o 2 Content, SOD, POD, CAT, PAO, DAO, PPO activity. Each sample was repeated 3 times and the average value was calculated.

[0044] The result is as figure 1 Shown, compound microbial agent T1 of the present invention, positive control group Bacillus compound bacterial agent T2 all significantly improved the activity of CAT, SOD, POD, PAO, DAO, PPO in Angelica blade compared with negative control group CK, and H 2 o 2 , chlorophyll content, significantly reduced the malondialdehyde content (P<0.05) in the leaves, and both treatments have played a better stress protection effect; compared with the positive control group Bacillus composite bacterial agent T2, the present invention After the compound microbial agent T1 treatment, the activities of CAT, POD, PAO and...

Embodiment 3

[0045] The influence of embodiment 3 different treatments on Angelica sinensis endogenous hormone

[0046] According to the method reported in the literature "Effects of Nano-iron and Melatonin on the Yield and Quality of Gansu Fritillaria Under Domesticated Cultivation Conditions" (Chinese Journal of Experimental Formulas, 2021), UPLC-MS was used to determine the contents of different endogenous hormones in the leaves of Angelica sinensis, Each sample was repeated 3 times and the average value was calculated.

[0047] The result is as shown in table 2, except salicylic acid, auxin, gibberellin, composite microbial agent T1 of the present invention, positive control group Bacillus composite bacterial agent T2 are more than negative control group CK, other hormone two All showed a consistent trend of change, significantly increasing the content of jasmonic acid, which increased by 257.91% and 99.94% respectively; reducing the content of abscisic acid, which decreased by 64.69% ...

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Abstract

The invention belongs to the field of microorganisms and biotechnology, and in particular relates to a compound bacterial agent of microorganisms and its application in increasing production and quality of Angelica sinensis against diseases. The compound microbial agent is composed of Pseudomonas fluorescens, Pseudomonas alcaligenes and Pseudomonas psychrophilus; it has ACC deaminase activity, SOD activity, POD activity, NEX neutral xylan Enzyme activity, chitinase activity, and laccase activity; can promote the growth of the roots of the underground medicinal organs of Angelica sinensis, increase the diameter of the reed head, the length of the reed head, the length of the main root, and the average weight per plant, not only increasing the production but also improving the quality of commercial Angelica sinensis. Appearance properties; can increase the content of ferulic acid, Z-ligustilide, ligustilide A, endogenous jasmonic acid, salicylic acid, etc., which can significantly improve the quality of Angelica sinensis, and can be used to prepare special microorganisms for Angelica sinensis Fertilizer; it can prevent and control plant diseases caused by pathogenic fungi of the genus Fusarium, and can be used to prepare pesticides for preventing and controlling plant diseases.

Description

technical field [0001] The invention relates to the field of microorganisms and biotechnology, in particular to a compound bacterial agent of microorganisms and its application in increasing production and quality of Angelica sinensis against diseases. Background technique [0002] Academician Huang Luqi of the Chinese Academy of Chinese Medical Sciences and Guo Lanping, a researcher team, proposed the "ecological planting of Chinese herbal medicines" model, which not only pays attention to the ecological conditions of the site, but also pays more attention to the input of agricultural inputs. It is the main mode of Chinese herbal medicines production at present. Pay attention to waste recycling and species diversity, do not use chemically synthesized fertilizers, pesticides, and plant growth regulators, and promote the use of animal sources, plant sources, microbial sources, natural minerals and other products are its basic requirements. [0003] Plant growth-promoting rhiz...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C05G3/00C05G3/60A01N63/27A01P3/00A01P21/00C12R1/39C12R1/38
CPCC12N1/20C05F11/08C05G3/00C05G3/60A01N63/27
Inventor 杨涛李有忠王治业杨晖王引权姚阳阳赵疆李鑫
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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