A strain of Streptomyces producing chitinase and its application

A chitinase, streptomyces technology, applied in applications, bacteria, microorganism-based methods, etc., can solve problems such as hidden dangers of pesticide residues, and achieve the effect of not producing drug resistance

Active Publication Date: 2021-08-17
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of hidden dangers of pesticide residues in the existing methods for controlling soybean gray mold and black spot, and provide a strain of streptomyces producing chitinase and its application

Method used

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  • A strain of Streptomyces producing chitinase and its application
  • A strain of Streptomyces producing chitinase and its application
  • A strain of Streptomyces producing chitinase and its application

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specific Embodiment approach 1

[0021] Specific embodiment one: a strain of chitinase-producing streptomyces in this embodiment is streptomyces (Streptomycessp.) G6, which is preserved in the General Microbiology Center of China Committee for the Collection of Microbial Strains, and the preservation address is Beichen West, Chaoyang District, Beijing No. 3, Courtyard No. 1, the preservation date is February 02, 2018, and the preservation number is CGMCCNo.15334.

[0022] In this embodiment, the wild rice rhizosphere soil is used to separate and purify chitinase-producing strains:

[0023] Preliminary screening: Using the plate dilution coating method, weigh 5g of the rhizosphere soil sample from Dongxiang wild rice in Jiangxi, put it in 45ml of sterile water, 120rpm, let it stand for 20min, and take the supernatant for 10-fold concentration gradient dilution. Take 10 respectively -1 、10 -2 、10 -3 80 μL of each concentration of diluent was applied to 3 chitin medium plates for each gradient, and sterile wa...

specific Embodiment approach 2

[0027] Embodiment 2: The application of a strain of Streptomyces producing chitinase in this embodiment refers to the application in the prevention and control of soybean diseases.

[0028] This embodiment provides a strain of Streptomyces G6 that has chitinase activity and has inhibitory effects on soybean gray mold and black spot in wild rice rhizosphere soil. The preservation number is CGMCC NO.15334, which is in In the rhizosphere soil of wild rice in different locations in Dongxiang, Jiangxi, strains with strong ability to decompose chitin were obtained through primary selection, and then the strains with good antagonistic effects against soybean gray mold and black spot were screened repeatedly. After a large number of experiments It has been proved that the strain G6 not only has chitinase activity, but also has a good antagonistic effect on soybean gray mold and black spot. Reached 85.5%.

specific Embodiment approach 3

[0029] Embodiment 3: This embodiment is different from Embodiment 2 in that: Streptomyces producing chitinase is used to suppress black spot. Others are the same as in the second embodiment.

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Abstract

A strain of streptomyces producing chitinase and its application, the invention relates to a strain of streptomyces producing chitinase and its application. The purpose of the present invention is to solve the problem that existing methods for controlling soybean gray mold and black spot have hidden dangers of pesticide residues. A strain of chitinase-producing Streptomyces of the present invention is Streptomyces (Streptomyces sp.) G6 , preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is February 2, 2018, and the preservation number is CGMCC No.15334. The application of the chitinase-producing streptomyces of the present invention refers to the application in the prevention and control of soybean diseases. Strain G6 not only has chitinase activity, but also has good antagonistic effect on soybean gray mold and black spot, has no pesticide residue, and does not produce drug resistance. The invention is applied to the technical field of environmental microorganisms.

Description

technical field [0001] The invention relates to a strain of streptomyces producing chitinase and its application. Background technique [0002] Soybean is the main food crop in my country, and it is also the main economic crop and feed crop. With the continuous growth of the population and the improvement and improvement of living standards, the yield and quality of soybeans are the focus of attention. Botrytis cinerea and black spot of soybeans can infect soybeans throughout the growth period, causing soybean diseases, and are distributed in Jilin, Liaoning, Heilongjiang, Jiangsu, Sichuan and other provinces. According to existing reports, soybean gray mold and black spot not only infect soybeans, but also have pathogenicity on plants such as ginseng, citrus, sweet potato, cabbage, jujube fruit, and rose. [0003] At present, the main method of controlling soybean gray mold and black spot is still based on chemical pesticides, but in view of the hidden dangers of pesticid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/28A61P3/00C12R1/465
CPCA01N63/10C12N1/205C12R2001/465
Inventor 田春杰田磊吉丽谢金宏陈德国张嘉旭张建峰马丽娜石少华罗莎莎常晶晶常春玲徐尚起
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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