Immune cell cryopreservation liquid, and preparation method, cryopreservation method and application thereof

Abstract

The invention discloses an immune cell cryopreservation liquid, and a preparation method, a cryopreservation method and an application thereof; the immune cell cryopreservation liquid includes the components by weight: 5%-15% of DMSO, 1%-5% of propylene glycol, 2%-8% of trehalose, 3%-8% of methyl cellulose, 0.3%-1.2% of glutathione, 0.06%-0.14% of sodium pyruvate, and 60%-80% of human donor plasma; the cryopreservation effect is quite good, the immune cells can be effectively protected from freezing injury, physiological functions and biological characteristics of the immune cells after resuscitation are maintained, cell biological characteristics are not changed, and the immune cell cryopreservation liquid can be used in clinical treatment; the immune cell cryopreservation liquid is suitable for long-term frozen preservation of human mononuclear cells and other kinds of immune cells.

Description

technical field [0001] The invention belongs to the technical field of cell preservation, and in particular relates to an immune cell cryopreservation solution, its preparation method, cryopreservation method and application. Background technique [0002] In recent years, immunotherapy techniques for tumors and viruses have been gradually applied clinically. Such as tumor vaccines and adoptive immunotherapy, these methods mainly improve the immunity of patients through in vitro stimulation, culture, expansion and reinfusion of autologous immune cells. At present, such cells mainly include dendritic cells (DC), cells Factor-induced killer cells (CIK), natural killer cells (NK), dendritic cell-induced killer cells (DC-CIK), chimeric antigen receptor T cells (CAR-T), etc. The initial cell sources of these induced and expanded immune cells are mononuclear cells from peripheral blood or umbilical cord blood, and they are directly isolated and cultured. [0003] As the age incre...

AI Technical Summary

Problems solved by technology

At present, the cryopreservation solution commonly used in China is basically DMSO, human donor serum, and immune cell basal medium. For example, CN104026118A discloses a kind of immune cell cryopreservation solution, its preparation method and application. Made of 10% dimethyl sulfoxide, 10% human donor plasma and 80% opTmizer TM CTS TM Composed of T-CellExpansion SFM medium, the immune cell cryopreservation solution does not contain animal serum, so it will not introduce foreign proteins, reduce the possibility of animal pathogen contamination, and will not affect human adoptive immunotherapy, but the freezing low immune cell survival

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