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176 results about "Recombinant Human Epidermal Growth Factor" patented technology
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Nepidermin (brand name Easyef), also known as recombinant human epidermal growth factor (rhEGF), is a recombinant form of human epidermal growth factor (EGF) and a cicatrizant (a drug that promotes wound healing through formation of scar tissue). It was developed by Daewoong Pharmaceutical.
Hydrolyzed silk protein/ recombinant human Epidermal Growth Factor (rhEGF) compound skin care facial mask and preparation method thereof
ActiveCN102357070AAnti agingPrevents and Reduces Fine LinesCosmetic preparationsToilet preparationsWrinkle skinAdditive ingredient
The invention discloses a hydrolyzed silk protein / recombinant human Epidermal Growth Factor (rhEGF) compound skin care facial mask and a preparation method thereof. The hydrolyzed silk protein / rhEGF compound skin care facial mask is prepared by components of 18 materials, and comprises two main components including, by weight, 1.5% to 2.5% of hydrolyzed silk protein and 0.01% to 0.1% of rhEGF. The skin care facial mask utilizes natural silk which contains a plurality of amino acids and proteins, and has the skin care effect of whitening, moisture preservation, skin smoothing, skin ageing retarding and the like. Each piece of silk facial mask converges rich silk protein essence, rhEGF and whitening moisture preservation components, provides dense whitening moistening for the skin, instantly brightens the skin color, enables the skin to be white and tender, solves the problems of withered and yellow skin color, dryness, roughness, fine wrinkles, elasticity lacking, thick pores and thelike, retains corneum moisture and integrity of a fiber structure, enables dark skin to reproduce silky smoothness and regain the touching color.
Owner:SHENZHEN GENE BIOLOGICAL TECH
Hydrogel dressing containing recombinant human epidermal growth factor and preparation method and application thereof
InactiveCN102078636AGood biocompatibilityMild preparation conditionsPeptide/protein ingredientsMetabolism disorderCross-linkTreatment effect
The invention discloses a hydrogel dressing containing a recombinant human epidermal growth factor. The hydrogel dressing consists of 100 volume parts of mixed solution A and 2 to 8 volume parts of cross-linking agent; the mixed solution A is prepared by mixing 2.0 to 5.0 mass percent of polyvinyl alcohol aqueous solution and 0.5 to 2.0 mass percent of sodium alginate aqueous solution according to the mass ratio of (2-5): 1, and adding 0 to 0.1 mass percent of sodium benzoate, 5 to 20ug / g of recombinant human epidermal growth factor and 2.0 to 10 mass percent of glycerol; and the gel dressing is prepared by taking the 2 to 8 volume parts of cross-linking agent prepared by mixing sodium borate solution and calcium chloride solution, dripping the cross-linking agent into the 100 volume parts of mixed solution A, standing and defoaming to obtain the gel dressing. The dressing has good biocompatibility and mild and simple preparation condition, can be formed at room temperature, can effectively keep the activity of the recombinant epidermal growth factor and has controlled release effect on the recombinant epidermal growth factor. The dressing has good effect of treating the wound of rats suffering from diabetes mellitus, and can accelerate healing of the wound.
Owner:SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
Escherichia expression system of secretory expression recombinant human epidermal growth factor
This invention includes signal peptide gene phoAspL10, expression plasmid pBL10EGF, host cell of bacillus coli BL21 (DE3) inverted by expression plasmid pBL10EGF, engineering strain BE-2 screened from the host cell and preparation method of rhEGF. The strain BE-2 can excrete and express rhEGF directly into culture after fermentation and the quantity is 384mg / l.The purity is more than 95% and specific activity more than 1.0*106IU / mg protein. The product rhEGF reserves crude space configuration and shows high biological activity.
Owner:SHANGHAI HAOHAI BIOLOGICAL TECH
External gel used for treating diabetic foot, and preparation method and application thereof
ActiveCN102949709AWon't scabPromote dissolutionOrganic active ingredientsNervous disorderGel preparationPh regulation
The invention relates to an external gel used for treating diabetic foot, and a preparation method and an application thereof. Specifically, the invention relates to an external gel preparation with oxygen-carrying and antibacterial materials, and containing recombinant human epidermal growth factor (rhEGF). The preparation basically comprises: a medicine component recombinant human epidermal growth factor (rhEGF), an oxygen-carrying material, an antibacterial material, a gel substrate, a stabilizing agent, a humectant, a penetration enhancing agent, a surfactant, and a pH regulation agent.
Owner:ZHANGJIAGANG IND TECH RES INST CO LTD DALIAN INST OF CHEM PHYSICS CHINESE ACADEMY OF SCI
External composition, and preparation and application thereof
ActiveCN103182070AStickyAvoid secondary cross-infectionPeptide/protein ingredientsAerosol deliveryOfficinalChlorhexidine
The invention relates to an external composition, and a preparation and an application thereof and belongs to the field of medicines. The external composition provided by the invention is a medicine composition which contains chlorhexidine or officinal salt thereof, or benzalkonium bromide or officinal salt thereof and a recombinant human epidermal growth factor or a recombinant cattle alkali fibroblast growth factor. According to the invention, the medicine composition is also further prepared into gel, liniment, ointment or paste, and the gel further comprises chitosan or officinal salt thereof and hyaluronic acid or officinal salt thereof. When being used for treating vagina inflammation, the external composition disclosed by the invention can improve a vagina microenvironment, promote growth of granulation tissue and repair vagina micro damage; and when being used for skin wound atraumatic restorative treatment, the external composition also can speed up restoration and regeneration of a wound tissue, promote healing and reduce scar formation, so that the external composition has a wide medical application prospect.
Owner:JIANGSU DIWO BIOLOGICAL PROD
Method for preparing dissolvable high activity recombinant human epidermal growth factor
ActiveCN101353659AHigh activityHigh purityPeptide preparation methodsFermentationEscherichia coliChemical synthesis
The invention relates to the technical field of gene recombination, in particular to a method for preparing soluble and high-activity recombinant human epidermal growth factor; the invention solves the problems that the human epidermal growth factor which is obtained by using the chemical synthesis method and the biological extraction method which have different self-defects respectively and by using the gene recombination method has lower purify and low yield and activity, and comprises the following steps of: constructing the gene cloning of the human epidermal growth factor, replacing codons which are not beneficial to be expressed by Escherichia coli to obtain optimized gene sequence, adding molecular chaperone of PDI to help target gene to be expressed effectively in Escherichia coli, and embedding HIS label and enzyme cutting site of protein in the sequence to help the target gene to be purified effectively, and the identification of biological activity; the obtained human epidermal growth factor has high purify, high yield, high activity, low cost, simple production technique and stable product quality; the product can be used for manufacturing drugs or articles for beauty and cosmetics and is beneficial to be promoted to the public.
Owner:SHANXI JINBO BIO PHARMA CO LTD
Wrinkle removing, anti-aging, skin activating and scar fading repair essence and preparation method thereof
InactiveCN104666114ARevitalize the skinLighten scarsCosmetic preparationsToilet preparationsWrinkle skinHydroxyproline
The invention discloses wrinkle removing, anti-aging, skin activating and scar fading repair essence which comprises components in parts by weight as follows: 600-850 parts of sterile water, 60-200 parts of glycerin, 30-80 parts of sodium pyrrolidone carboxylic acid, 30-80 parts of 1,3-butanediol, 3-10 parts of collagen, 5-20 parts of panthenol, 3-10 parts of allantoin, 10-40 parts of hydroxyproline, 2-10 parts of bisabolol, 2-10 parts of hyaluronic acid, 0.5-2 parts of recombinant human epidermal growth factors and 1-5 parts of diazolidinyl urea. The preparation method comprises the following steps: (1), sterile water, glycerin, sodium pyrrolidone carboxylic acid, 1,3-butanediol and collagen are mixed uniformly with allantoin and are heated for 20 min at 85 DEG C; (2), the mixture is stirred for 20 min at the speed of 30 rpm and is cooled; (3), panthenol and hydroxyproline are added when the temperature is 55 DEG C, the mixture is uniformly mixed, residual components are added when the temperature is 40 DEG C, the mixture is uniformly mixed and then homogenized for 30s, and discharging is performed when the temperature is 30 DEG C, and the repair essence is obtained.
Owner:成都舒乐科技发展有限公司
Serum-free culture medium, preparation method thereof and culture method of mesenchymal stem cells
ActiveCN110923196AImprove proliferative abilityImprove performanceCulture processSkeletal/connective tissue cellsPolyvinyl alcoholHydrocortisone
Owner:GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
Method for producing recombinant human epidermal growth factor acceptor 2 cytoplasmic region with methylotrophy yeast
InactiveCN101492675AEfficient expressionOptimizing Dissolved Oxygen LevelsFungiMicroorganism based processesYeastFermentation
The invention relates to a method for producing, purifying and appraising protein, in particular to a method for expression of recombinant human her2 / neu ICD in Pasteur yeast as well as optimized large scale industrialized fermentation production and purification of the recombinant human her2 / neu ICD.
Owner:吉林圣元科技有限责任公司
Stem cell culture medium
InactiveCN105200008ADoes not affect potentialHigh speedNervous system cellsSkeletal/connective tissue cellsSodium bicarbonateHuman platelet
The invention discloses a stem cell culture medium. The stem cell culture medium comprises an improved DMEM (Dulbecco's Modified Eagle Medium) / F12 basal culture medium and an additive, wherein the additive comprises sodium bicarbonate, selenium amino acid chelate, recombinant human insulin growth factors, recombinant human basic fibroblast growth factors, recombinant human lactoferrin, ascorbic acid, recombinant human platelet-derived growth factors, recombinant human vascular endothelial cell growth factors, octacosanol, polyvinyl alcohol, polyvinylpyrrolidone and recombinant human epidermal growth factors. According to the stem cell culture medium, the potential of stem cells is not influenced while the stem cells can be proliferated rapidly, the proliferation speed of the stem cells is increased by 3-5 times compared with a common culture medium, and further, the stem cell culture medium can be used for culturing the stem cells of various kinds of tissue and has excellent applicability; the cultured stem cells have high differentiation capability, can be differentiated into multiple functional cells and have very high scientific research and medical application values, culture medium components are exact, the quality is stable, and accordingly, the cultured stem cells are not likely to generate human body rejection reaction after transplanting.
Owner:XINXIANG MEDICAL UNIV
Serum-free culture medium used for full-suspension culture of MDCK (Madin Darby Canine Kidney) cells
The invention provides a serum-free culture medium used for full-suspension culture of MDCK (Madin Darby Canine Kidney) cells. The culture medium is prepared from a DMEM / F12 culture medium containing 5g / L of NaCl and the following components: sodium stannite, recombinant human epidermal growth factors, hydrocortisone, recombinant full-chain insulin, prostaglandin E1, human transferrin, thyroxine (T3), vitamin E, cholesterol, ethanol amine, beta-mercaptoethanol, Tween-80, Hypep1510, recombinant human serum albumin ACF and mannitol. With the adoption of the culture medium provided by the invention, the full-suspension culture of the MDCK cells can be carried out very well under the condition that serum is not added; after the MDCK cells are continuously cultured for 20 generations, the average proliferation concentration of the MDCK cells is 2.308*10<6> / mL, the average cell viability is 97.4 percent and the average doubling time is 34.48 hours, so that the serum-free culture medium provides technical supports for developing influenza vaccines of cell matrixes of mammals in China.
Owner:马忠仁 +2
Neural stem cells medium and method for performing human neural stem cells in-vitro long-term culture and amplification by using neural stem cells medium
ActiveCN105062972AGenetic stabilitySolve the easy differentiation of in vitro cultureNervous system cellsInsulin activityCuticle
The invention relates to a neural stem cells medium and a method for performing human neural stem cells in-vitro long-term culture and amplification by using the neural stem cells medium. The neural stem cells medium comprises the following ingredients by weight proportion: 100-1000 micrograms of heparin sodium, 10-100 micrograms of vitamin E, 5-50 milligrams of insulin human recombinant, 0.5-5 milligrams of putrescine, 2-10 micrograms of sodium selenite, 2-10 milligrams of human transferrin, 2-10 micrograms of progestin, 300 milligrams of L-glutamine, 5.9 grams of 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid, 10-100 micrograms of recombinant human epidermal growth factors, 10-100 micrograms of recombinant human basic fibroblast growth factors, 20-200 milligrams of vitamin C glucoside and 40,000-400,000 IU (international unit) of gentamicin. By the neural stem cells medium, the technical problems that human neural stem cells are easy to differentiate when cultured in vitro and long-term culture and amplification are difficult to implement are solved.
Owner:ZHEJIANG ORIGIN BIOTECH
Serum-free medium for liver cancer stem cells and culture method for serum-free medium
The invention provides a serum-free medium for liver cancer stem cells and a culture method for the serum-free medium. Based on a DMEM / F12+GlutaMAXTM<-1> medium, the serum-free medium comprises a recombinant human epidermal growth factor, a recombinant human basic fibroblast growth factor-10, an insulin No.1 growth factor, a serum-free complementary nutritional ingredient, heparin and a penicillin and streptomycin double-resistant solution. The serum-free medium has the effects as follows: 1, three special growth factors are added to the serum-free medium in a corresponding proportion; direct serum-free culture is realized, so that liver cancer cells can be pelletized, the pelletizing rate is high, and continuous and stable passage can be realized; 2, the growth factors in the serum-free medium keep a small group of cells in an undifferentiated state; the serum-free medium and the culture method have wide prospect in fundamental research and clinical application of serum-free culture of the liver cancer stem cells and provide rich experiment materials for research of the liver cancer stem cells.
Owner:广西壮族自治区肿瘤医院
Separation and cultivation method of human adipose stem cells
ActiveCN108220230ANormal growthNormal metabolismCell dissociation methodsCulture processSerum free mediaParenchyma
The invention discloses a separation and cultivation method of human adipose stem cells. The method comprises the following steps: first, digesting a fat suction matter by using a Liberase digestive enzyme solution, neutralizing digestive enzyme after digestion is ended, centrifuging, filtering, and further removing parenchyma cells by using a lymphocyte separation solution so as to obtain the human adipose stem cells; second, cultivating the human adipose stem cells by using a serum-free medium, wherein following components are added in the serum-free medium: a recombinant human epidermal growth factor, a recombinant human basic fibroblast growth factor, a recombinant human transforming growth factor-beta, a recombinant human platelet-derived growth factor-BB, a recombinant human stem cell factor, reduced glutathione, coenzyme A, biotin, MEM vitamin solution, MEM amino acid solution, MEM non-essential amino acid solution, a GlutaMAX additive, insulin-transferrin-selenium solution andgentamicin. The method can remarkably increase the yield of adipose stem cells and improve the activity of the adipose stem cells, so as to obtain more adipose stem cells with proliferation ability.
Owner:SHANGHAI LIFE SCI & TECH CO LTD
Construction and screening method for recombinant human epidermal growth factor engineering bacteria
InactiveCN102978231AGuaranteed homogeneityImprove stabilityBacteriaMicroorganism based processesChemical synthesisEscherichia coli
The present invention provides a construction and screening method for recombinant human epidermal growth factor (hEGF) engineering bacteria. The construction and screening method comprises the following main steps: 1, adopting a PCR technology to obtain kanamycin resistance gene, and adopting a chemical synthesis method to obtain an escherichia coli cspA gene function element; 2, respectively optimizing gene sequences encoding SUMO protein and hEGF, and then adopting chemical synthesis to obtain a large amount of expressed fusion protein gene sequences OPT-SUMO-hEGF; 3, linking the fragments obtained from the previous two steps into pET21 plasmid to obtain pET-Kan-cspA-SE plasmid; 4, adopting the pET-Kan-cspA-SE plasmid as a template to construct a transposition body; 5, transforming the transposition body into escherichia coli Origami 2 (DE3); and 6, screening a strain having high fusion protein SUMO-hEGF expression. According to the present invention, genome of the obtained strain is stable, a soluble rate of the expressed hEGF is high, and the method is suitable for large-scale industrial production.
Owner:天津强微特生物科技有限公司
Human umbilical cord mesenchymal stem cell serum-free culture medium and preparation method thereof, and acquisition method of human umbilical cord mesenchymal stem cell serum-free culture solution
ActiveCN110564680AAvoid unsafe factors such as immune reactionsPromotes adherent growthCulture processSkeletal/connective tissue cellsSodium bicarbonateCuticle
The invention discloses a human umbilical cord mesenchymal stem cell serum-free culture medium and a preparation method thereof, and an acquisition method of a human umbilical cord mesenchymal stem cell serum-free culture solution. The culture medium comprises a basic culture medium and a serum-free culture supernatant concentrated solution, and also comprises the following components and concentrations: 5-100 ng / ml of a vitamin composition, 50-200 [mu]g / ml of grape polyphenol, 1-50 ng / ml of a recombinant human epidermal growth factor, 1-50 ng / ml of a recombinant human basic fibroblast growthfactor, 1-10 ng / ml of a recombinant platelet-derived growth factor-C, 1-10 ng / ml of a recombinant human insulin-like growth factor-1, 50-200 [mu]M of beta-mercaptoethanol, 1-100 [mu]g / ml of human serum albumin, 1-100 [mu]g / ml of glutathione, and 1.2-3.7 g / L of sodium bicarbonate. The human umbilical cord mesenchymal stem cell serum-free culture medium is used for replacing a traditional culture medium containing animal serum to culture human umbilical cord mesenchymal stem cells, and unsafe factors such as human immune response caused by stem cell pollution by pathogens or animal-derived components and heterologous protein are avoided.
Owner:西安艾尔菲生物科技有限公司
Wrinkle-removing, anti-ageing, skin-activating, restoring and whitening composition
InactiveCN103520017ATo promote metabolismIncrease elasticityCosmetic preparationsToilet preparationsCuticleGlycerol
The invention discloses a wrinkle-removing, anti-ageing, skin-activating, restoring and whitening composition. The composition comprises the following components in percentage by weight: 15%-30% of hyaluronic acid, 25%-40% of collagen, 0.0001%-0.0009% of recombinant human epidermal growth factor, and 5%-20% of glycerol. Collagen can effectively inhibit generation of melanin and promote metabolism of skin tissue; hyaluronic acid can effectively nourish and moisturize skin, remove wrinkles and beautify looks; recombinant human epidermal growth factor can quicken dropping of senescent cells of a human body and promote reverse differentiation of the cells to generate young cells, can promote regeneration of subcutaneous collagen and recombination of fibrous tissue, improve skin elasticity and reduce skin sensitivity, and can make thick, flushing and expanding facial capillaries atrophy and get thinner and shallower, avoid pigmentation and remove facial toxin residues so as to achieve the efficacy of removing freckles, acne marks and wrinkles as well as tendering and whitening.
Owner:易金阳
Novel preparation method for recombinant human epidermal growth factor
ActiveCN102757974AOvercoming difficulties in expressing independentlyHigh expressionPeptide preparation methodsVector-based foreign material introductionIon chromatographyProtein target
The invention aims to provide a simple and inexpensive preparation method for an Ssp intein system mediated recombinant human epidermal growth factor. According to the method, the conventional column cutting and chitin affinity chromatography are replaced with liquid phase cutting and ion exchange chromatography. The column cutting adopted in the conventional Ssp intein system mediated protein purification method is replaced with the liquid phase cutting, so that the loss of the target protein caused by the automatic splitting of fusion protein is reduced; and the expensive chitin affinity chromatography is replaced with the ion exchange chromatography, so that the purification cost of the recombinant human epidermal growth factor is greatly reduced, the operation steps are simplified, and the bottleneck of the large-scale industrial production of the recombinant human epidermal growth factor is overcome.
Owner:MICROBIOLOGY INST OF SHAANXI
Umbilical cord mesenchymal stem cell serum-free cultivation method and application
InactiveCN105602893ANo pollution in the processMaintain propertiesCell culture mediaCell culture supports/coatingDiseaseCuticle
The invention discloses an umbilical cord mesenchymal stem cell serum-free cultivation method, comprising the following steps: (1) preparing coating buffer for fibronectin, a recombinant human epidermal growth factor and a recombinant human fibroblast growth factor, and coating a plastic culture bottle; (2) taking human umbilical cord tissue Whartonps jelly, cutting into pieces, and adding into the culture bottle in the step (1); (3) for a product obtained in the step (2), adding into a serum-free culture medium; (4) putting into a 5-percent carbon dioxide incubator at 37 DEG C for culture, wherein mesenchymal stem cells overgrow in 10-12 days; (5) washing by a phosphate buffer solution, and performing Triple enzymic digestion; (6) adding the phosphate buffer solution, and stopping the digestion; (7) collecting mesenchymal stem cell supernate; (8) centrifuging; (9) collecting sediments to obtain the needed mesenchymal stem cells. By adopting the method, the mesenchymal stem cells can go down to the 20th generation, the characteristics of the mesenchymal stem cells are kept, diseases can be treated, and application prospect is achieved.
Owner:SHANGHAI JIQUAN BIOTECH CO LTD
Composition capable of promoting differentiation of autologous preadipocytes and formation of lipid droplets and preparation method thereof
ActiveCN103055303APromote differentiationPromote formationPeptide/protein ingredientsSexual disorderLipid formationCuticle
The invention specifically discloses a composition capable of promoting differentiation of autologous preadipocytes and formation of lipid droplets and a preparation method thereof. A commiphora mukul resin extract and a recombinant human epidermal growth factor (rhEGF) are main components of the composition; and the content of the components are that the commiphora mukul resin extract is 1.0 to 3.0% and the recombinant human epidermal growth factor (rhEGF) accounts for 0.01 to 0.10%. According to the invention, the commiphora mukul resin extract and the recombinant human epidermal growth factor are organically combined together; on the one hand, differentiation of autologous preadipocytes is promoted by the recombinant human epidermal growth factor, on the other hand, activity of lipase in decomposition of fat is inhibited by the commiphora mukul resin extract, so formation and accumulation of lipid droplets are promoted, and thus, the volume of a breast can be effectively increased.
Owner:SHENZHEN GENE BIOLOGICAL TECH
Skin care cosmetic taking American wild yam extract as main component and preparation method thereof
ActiveCN102085172AFor comprehensive skin carePromote regenerationCosmetic preparationsToilet preparationsCuticleVitality
The invention discloses a skin care cosmetic taking an American wild yam extract as a main component and a preparation method thereof. The skin care cosmetic taking the American wild yam extract as the main component is prepared by five phases of materials, wherein the most important two components by weight percent are as follows: the American wild yam extract accounts for 2.0-8.0% and a rhEGF (recombinant human epidermal growth factor) accounts for 1-0.10%. The skin care cosmetic organically combines the rhEGF with American wild yam essence from Mexico, on the one hand, damaged cells can be repaired, and senile cells can be activated, thereby enabling skin cells to have sufficient vitality to absorb and utilize nutritional substances and promoting regeneration and health of the cells; on the other hand, a sufficient quantity of hormone precursor can be provided for skin, and the endogenous aging problem of the skin can be effectively suppressed and prevented after skin absorption and utilization, thereby being the skin care cosmetic which can realize percutaneous absorption through in vitro application and play a comprehensive skin care effect.
Owner:SHENZHEN GENE BIOLOGICAL TECH
Recombinant human epidermal growth factor cationic liposome and preparation method thereof
InactiveCN102949345AImprove tissue compatibilityIncrease cell affinitySenses disorderPeptide/protein ingredientsNiosomeCitrate buffer
The invention relates to a recombinant human epidermal growth factor cationic liposome and a preparation method thereof. The recombinant human epidermal growth factor cationic liposome comprises a recombinant human epidermal growth factor, a cationic liposome and a growth factor protective agent, wherein a part of the recombinant human epidermal growth factor is encapsulated by the cationic liposome. The preparation method comprises the following steps of: step 1. dissolving a panniculus adiposus material and a cationic additive by an absolute ethyl alcohol, carrying out rotary evaporation to get rid of the alcohol to form uniform panniculus adiposus, and adding a citrate buffer solution and hydrating to obtain a crude liposome suspension liquid; step 2. carrying out ultrasonic dispersion to process the crude liposome suspension liquid, and straightening particles by microfiltration membranes to obtain the blank liposome; and step 3. adding the blank liposome solution after mixing a recombinant human epidermal growth factor solution with a growth factor protective agent solution, adjusting pH to 6.0-8.0, and carrying out incubation processing to obtain the recombinant human epidermal growth factor cationic liposome. According to the preparation method, the human epidermal growth factor can be encapsulated by the cationic liposome so that the stability of rhEGF, the intermiscibility of tissues and the affinity of cells can be increased.
Owner:SHENZHEN POLYTECHNIC
Induced gel used for repairing skin wound surfaces or cervical mucosa
ActiveCN101780031AEffective suspensionNo side effectsPeptide/protein ingredientsPharmaceutical delivery mechanismTriclosanEcological environment
The invention relates to a gel, in particular to an induced gel used for repairing skin wound surfaces or cervical mucosa, which not only can solve the therapy problems of skin wounds, cuts and ulcer wound surfaces but also can improve the micro-ecological environments of vaginae and repair the microdamage of the vaginae and meanwhile has good therapeutic effect on cervical erosion. The induced gel comprises Carbomer, triclosan and a recombination human epidermal growth factor, wherein the concentration of Carbomer is 3 to 5 percent, the concentration of triclosan is 0.1 to 0.6 percent, and the concentration of the recombination human epidermal growth factor is 0.00001 to 0.0005 percent. The induced gel can improve the micro-ecological environments of the vaginae and repair the microdamage of the vaginae, thereby being suitable for various vagina inflammations and cervicitis caused by vagina micro-ecological disorder and vagina damage and being suitable for the auxiliary therapy after the clinical therapy on vaginitis and cervicitis and the therapy on cervical erosion; the induced gel also can be used for treating the skin wounds, the cuts and the skin wound surfaces, thereby accelerating the repair and the regeneration of wound tissues, promoting healing and reducing scar formation.
Owner:山西锦正科技有限公司
Serum-free umbilical cord mesenchymal stem cell culture medium
InactiveCN110317779AIncrease growth rateMaintain propertiesCulture processSkeletal/connective tissue cellsVitamin CCuticle
The invention discloses a serum-free umbilical cord mesenchymal stem cell culture medium. The culture medium is used for culturing umbilical cord mesenchymal stem cells and comprises a serum-free basic culture medium and additive components, wherein the addition components include a recombinant human fibroblast growth factor (hFGF), a recombinant human epidermal growth factor (hEGF), insulin hI, L-glutamine, 2-mercaptoethanol, sodium selenite, transferrin, human serum albumin, fibronectin, astragalus polysaccharide APS, IL-3, IL-6, IL-r, a ginseng extract, a rose extract, a jasmine flower extract and vitamin C.
Owner:GUANGDONG AIE BIOSCIENCE CO LTD
Novel synergistic pharmaceutical composition for topical applications
ActiveUS20130085103A1Increasing antibacterial effectiveness of antibacterial agentImprove breathabilityAntibacterial agentsOintment deliveryDiseaseAdditive ingredient
A synergistic pharmaceutical composition for the preparation of topical formulations for use in prophylaxis and treatment of wounds, burn wounds, skin grafts, pressure ulcers, diabetic foot ulcers and other skin diseases is provided. The composition may include one or more synergistically active ingredients and one or more inactive ingredients. The synergistically active ingredients may include Recombinant Human Epidermal Growth Factor (rh-EGF) (REGEN-D™ of Bharat Biotech International Limited) and / or Platelet Derived Growth Factor (rh-PDGF-BB), silver sulfadiazine (SSD) and chlorhexidine gluconate (CHG). One or more inactive ingredients may comprise carriers, preservatives, emulsifiers, skin emollients and soothers and one or more other constituents.
Owner:BHARAT BIOTECH INTERNATIONAL
Bio-environment friendly antiviral cold prevention toothpaste and preparation method thereof
InactiveCN102727408ARelieve symptomsReduced incidence of coldsCosmetic preparationsToilet preparationsBiotechnologyDisease
The invention discloses a biological environmental antiviral cold prevention toothpaste and a preparation method thereof. The toothpaste contains the following components of: alkyl polyglucoside (APG), precipitated silica, lysozyme, neutral protease, hydrolyzed protein, immunoglobulin, recombinant human epidermal growth factor, strontium chloride, sorbitol, butylparaben, CMC (Carboxyl methyl Cellulose), xanthan gum, glucolase, enzyme, and a skin penetrating agent, etc. The invention is directed at the pathogenic mechanism that illness finds its way in by the mouth and nose, and the biologicalenvironmental antiviral cold prevention toothpaste with bio-environmental protection, virus resisting, cold prevention, as well as disease prevention and treatment effects is designed and prepared. During use, the toothpaste of the invention has cool, refreshing, icy and fragrant mouthfeel, and decomposes rapidly after entering the mouth. After tooth brushing, the ice-cold and refreshing feel remains for a long time, the bacteriostatic control time is long, the mouthfeel is refreshing, the taste is fragrant and bittersweet, and the spirit can be made cheerful, so that the toothpaste of the invention is beneficial to human health.
Owner:董家华
Essence liquid capable of activating skin vitality and restraining aging
ActiveCN105012208AStrong lockGood moisturizing effectCosmetic preparationsToilet preparationsMetaboliteDamages tissue
The invention discloses essence liquid capable of activating skin vitality and restraining aging. The essence liquid capable of activating the skin vitality and restraining aging comprises, by mass, 2,000,000-4,000,000 activity units / g of SOD, 3-9% of collagen, 3-5% of ceramide, 0.06-0.1% of recombinant human epidermal growth factor, 2-5% of chrysanthellum indicum extract, 1-3% of snail slime extract, 1-4% of B-glucan, 0.5-2% of hyaluronic acid, 4-8% of tocopherol, 1-2% of vitamin C and the balance mineral water. By means of the essence liquid capable of activating the skin vitality and restraining aging, blocked pores are unchoked, the sterilization and inflammation removing effects are achieved, water needed by skins is supplemented, activity and elasticity of cells are roundly enhanced, skin tightness degree is increased, dim metabolites on epidermis are removed, and the essence liquid can assist in healing damaged tissues and tightening the skins.
Owner:陈俊杰
Medicine composition for promoting wound healing after operation and preparation method thereof
InactiveCN104940905AAvoid damagePromote dissolutionPeptide/protein ingredientsDermatological disorderMomordicaCuticle
The invention discloses a medicine composition for promoting wound healing after operation and a preparation method thereof. The medicine composition is prepared from the following raw materials in parts by weight: 22-28 parts of natural indigo, 12-21 parts of radix notoginseng, 10-14 parts of Mongolian dandelion herb, 5-7 parts of Grosvenor momordica, 3-5 parts of liquorice root, 0.00005 part of recombinant human epidermal growth factor, 0.001 part of mannitol and 0.0005 part of human albumin. The medicine composition has obvious effects when being used clinically.
Owner:GUILIN PAVAY GENE PHARMA
Serum-free culture medium for mesenchymal stem cells
InactiveCN110257328APromote in vitro proliferationCulture processSkeletal/connective tissue cellsSodium bicarbonateLithium chloride
The invention provides a serum-free culture medium for mesenchymal stem cells. The serum-free culture medium specifically comprises a lipid mixture, glutamine, hydrocortisone, zinc sulfate, sodium butyrate, ferric citrate, vitamin C, recombinant epidermal growth factors, recombinant alkaline fibroblast growth factors, RGD oligopeptides, palmitoyl tripeptide-5, palmitoyl tetrapeptide-7, lithium chloride, L-glutathione, a soybean pancreatin inhibitor, beta-mercaptoethanol, ethanolamine, sodium selenite, Hepes, sodium bicarbonate and a basic culture medium. The serum-free culture medium is free of human and animal resources, clear in chemical component, rich in nutrition, capable of achieving UC-MSCs rapid proliferation, and capable of solving the problem of cells number insufficiency and maintaining the biological features and immunophenotyping stability of the mesenchymal stem cells.
Owner:GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD +1
Endothelium culture system
InactiveCN105647851AIncrease growth activityInhibitory activityCulture processArtificial cell constructsInsulin-like growth factorVitamin C
The invention discloses an endothelium culture system. The endothelium culture system comprises an endothelium culture medium and an endothelium additive, wherein the endothelium additive comprises components by concentration as follows: 5 ng / ml of a recombinant human epidermal growth factor, 10 ng / ml of hydrocortisone, 10 ng / ml of a recombinant human vascular endothelial growth factor, 5 ng / ml of a recombinant human basic fibroblast growth factor, 5 ng / ml of an R3 insulin-like growth factor, 50 mu g / ml of vitamin C, 50 mu g / ml of heparin, 10 mu g / ml of recombinant human insulin and 5 mu g / ml of human total iron transferrin. The endothelium culture system can promote growth activity of great vascular endothelial cells in a 5% serous environment, partially inhibit activity of fibroblasts and smooth muscle cells and reduce parenchyma cell pollution.
Owner:ICELL SHANGHAI BIOSCI CO LTD
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