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Serum-free umbilical cord mesenchymal stem cell culture medium

A kind of stem cell and culture medium technology, applied in the field of stem cell research, can solve the problems of easily changing cell morphology and biological properties, slow proliferation, and failure to meet the needs of clinical application of stem cells, so as to maintain the characteristics of stem cells and the rate of cell growth and proliferation high effect

Inactive Publication Date: 2019-10-11
GUANGDONG AIE BIOSCIENCE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solve the problem of slow proliferation of umbilical cord mesenchymal stem cells in the existing culture medium, easy changes in cell morphology and biological properties, and cannot meet the needs of clinical application of stem cells

Method used

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  • Serum-free umbilical cord mesenchymal stem cell culture medium
  • Serum-free umbilical cord mesenchymal stem cell culture medium
  • Serum-free umbilical cord mesenchymal stem cell culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1. Preparation of culture medium

[0020] The serum-free basic medium is DMEM / F12 medium;

[0021] The formula for adding ingredients is 1ng / mL recombinant human fibroblast growth factor, 1 μg / mL recombinant human epidermal growth factor, 1 μg / mL insulin, 1 mM L-glutamine, 50 μM 2-mercaptoethanol, 20 nM sodium selenite, 10 μg / mL ml transferrin, 10mg / mL human serum albumin, 50μg / mL fibronectin, 50ug / ml astragalus polysaccharide APS, 10mg / L IL-3, 10mg / LIL-6, 45μg / L IL-r, 10mg / L Ginseng extract, 10mg / L rose extract, 10mg / L jasmine flower extract, 5mM vitamin C;

[0022] The preparation method of the culture medium is as follows: according to the specified amount, the added ingredients are divided into a mother liquid, filtered through a 0.45um filter membrane, added with a serum-free basic medium, and mixed evenly to adjust to a final concentration.

Embodiment 2

[0023] Embodiment 2. Preparation of culture medium

[0024] The serum-free basic medium is DMEM / F12 medium;

[0025] The added ingredient formula is 20ng / mL recombinant human fibroblast growth factor, 20 μg / mL recombinant human epidermal growth factor, 20 μg / mL insulin, 10 mM L-glutamine, 200 μM 2-mercaptoethanol, 50 nM sodium selenite, 30 μg / mL ml transferrin, 100mg / mL human serum albumin, 200μg / mL fibronectin, 200ug / ml astragalus polysaccharide APS, 50mg / L IL-3, 50mg / L IL-6, 55μg / L IL-r, 50mg / L ginseng extract, 50mg / L rose extract, 50mg / L jasmine flower extract, 8mM vitamin C;

[0026] The preparation method of the culture medium is as follows: according to the specified amount, the added ingredients are divided into a mother liquid, filtered through a 0.45um filter membrane, added with a serum-free basic medium, and mixed evenly to adjust to a final concentration.

Embodiment 3

[0027] Embodiment 3. Preparation of culture medium

[0028] The serum-free basic medium is DMEM / F12 medium;

[0029] The added ingredient formula is 5ng / mL recombinant human fibroblast growth factor, 5 μg / mL recombinant human epidermal growth factor, 5 μg / mL insulin, 5 mM L-glutamine, 80 μM 2-mercaptoethanol, 25 nM sodium selenite, 15 μg / mL ml transferrin, 30mg / mL human serum albumin, 80μg / mL fibronectin, 80ug / ml astragalus polysaccharide APS, 50mg / L IL-3, 50mg / LIL-6, 55μg / L IL-r, 50mg / L Ginseng extract, 50mg / L rose extract, 50mg / L jasmine flower extract, 8mM vitamin C;

[0030] The preparation method of the culture medium is as follows: according to the specified amount, the added ingredients are divided into a mother liquid, filtered through a 0.45um filter membrane, added with a serum-free basic medium, and mixed evenly to adjust to a final concentration.

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Abstract

The invention discloses a serum-free umbilical cord mesenchymal stem cell culture medium. The culture medium is used for culturing umbilical cord mesenchymal stem cells and comprises a serum-free basic culture medium and additive components, wherein the addition components include a recombinant human fibroblast growth factor (hFGF), a recombinant human epidermal growth factor (hEGF), insulin hI, L-glutamine, 2-mercaptoethanol, sodium selenite, transferrin, human serum albumin, fibronectin, astragalus polysaccharide APS, IL-3, IL-6, IL-r, a ginseng extract, a rose extract, a jasmine flower extract and vitamin C.

Description

technical field [0001] The invention relates to the research field of stem cells, in particular to a novel, highly efficient serum-free medium suitable for large-scale cultivation of umbilical cord mesenchymal stem cells. Background technique [0002] Umbilical cord mesenchymal stem cells refer to a kind of pluripotent stem cells existing in the umbilical cord tissue of newborns. The cell surface strongly expresses specific markers CD90, CD73, and CD105, but does not express markers of hematopoietic or endothelial cells CD45, CD14, CD11, CD34, CD19, low expression or no expression of major histocompatibility antigen HLA-DR. Studies have shown that umbilical cord mesenchymal stem cells have high differentiation potential and can differentiate in multiple directions such as bone, cartilage, muscle, tendon, ligament, nerve, liver endothelium and cardiac muscle. Compared with bone marrow mesenchymal stem cells, the content and proliferation ability of umbilical cord mesenchymal...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0665C12N2500/12C12N2500/24C12N2500/32C12N2500/34C12N2500/44C12N2500/76C12N2501/11C12N2501/115C12N2501/23C12N2501/2303C12N2501/2306C12N2501/33C12N2501/998
Inventor 李玉霞邓广文吴曙霞
Owner GUANGDONG AIE BIOSCIENCE CO LTD
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