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Stem cell culture medium

A culture medium and stem cell technology, applied in the field of stem cell culture, can solve the problems of reduced functional cell potential and slow growth, and achieve the effect of clear composition, strong differentiation ability and stable quality

Inactive Publication Date: 2015-12-30
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, stem cells cultured in this medium not only grow slowly, but also have a greatly reduced potential to differentiate into functional cells after more than 5 passages

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A stem cell culture medium, including DMEM / F12 basal culture, vitamin C 1μg / ml, vitamin B family 30μg / ml, vitamin E 10μg / mL, L-glutamine 1mg / ml, manganese methionine 1mg / ml, zinc chloride 1mg / ml ml, amino acid chelated magnesium 1mg / ml, selenomethionine 1.5mg / ml, phytase 0.5mg / ml, chromium picolinate 0.055mg / ml, calcium iodate 0.1mg / ml, lysine 1mg / ml, trichloro Hexaammine cobalt 0.05mg / ml, protein germanium 0.55mg / ml, iodine yeast 2mg / ml, copper chloride 0.1mg / ml, sodium bicarbonate 400μg / ml, amino acid chelated selenium 8ng / ml, recombinant human insulin growth Factor 10ng / ml, recombinant human basic fibroblast growth factor 80ng / ml, recombinant human lactoferrin 7μg / ml, ascorbic acid 50μg / ml, recombinant human platelet-derived growth factor 3μg / ml, recombinant human vascular endothelial growth factor 1ng / ml, octacosanol 0.1 μg / ml, polyvinyl alcohol 0.1 μg / ml, polyvinylpyrrolidone 0.1 μg / ml, recombinant human epidermal growth factor 1 μg / ml.

Embodiment 2

[0020] A stem cell culture medium, including DMEM / F12 basal culture, vitamin C50μg / ml, vitamin B family 100μg / ml, vitamin E100μg / mL, L-glutamine 5mg / ml, manganese methionine 10mg / ml, zinc chloride 5mg / ml ml, amino acid chelated magnesium 5mg / ml, selenomethionine 5.5mg / ml, phytase 0.75mg / ml, chromium picolinate 0.065mg / ml, calcium iodate 0.3mg / ml, lysine 6mg / ml, trichlor Hexaammine cobalt 0.065mg / ml, protein germanium 0.75mg / ml, iodine yeast 6mg / ml, copper chloride 1mg / ml, sodium bicarbonate 500μg / ml, amino acid chelated selenium 14ng / ml, recombinant human insulin growth factor 20ng / ml, recombinant human basic fibroblast growth factor 100ng / ml, recombinant human lactoferrin 10μg / ml, ascorbic acid 60μg / ml, recombinant human platelet-derived growth factor 5μg / ml, recombinant human vascular endothelial growth factor 4ng / ml ml, octacosanol 5.05 μg / ml, polyvinyl alcohol 5.05 μg / ml, polyvinylpyrrolidone 5.05 μg / ml, recombinant human epidermal growth factor 5.5 μg / ml.

Embodiment 3

[0022] A stem cell medium, including DMEM / F12 basal culture, vitamin C25.5μg / ml, vitamin B family 65μg / ml, vitamin E55μg / ml, L-glutamine 3mg / ml, manganese methionine 5.5mg / ml, chloride Zinc 3mg / ml, amino acid chelated magnesium 3mg / ml, selenomethionine 3.5mg / ml, phytase 0.625mg / ml, chromium picolinate 0.06mg / ml, calcium iodate 0.2mg / ml, lysine 3.5mg / ml ml, hexaammine cobalt trichloride 0.0575mg / ml, protein germanium 0.65mg / ml, iodine yeast 4mg / ml, copper chloride 0.55mg / ml, sodium bicarbonate 600μg / ml, amino acid chelated selenium 20ng / ml, Recombinant human insulin growth factor 30ng / ml, recombinant human basic fibroblast growth factor 120ng / ml, recombinant human lactoferrin 13μg / ml, ascorbic acid 70μg / ml, recombinant human platelet-derived growth factor 7μg / ml, recombinant human vascular endothelium Cell growth factor 7ng / ml, octacosanol 10μg / ml, polyvinyl alcohol 10μg / ml, polyvinylpyrrolidone 10μg / ml, recombinant human epidermal growth factor 10μg / ml.

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Abstract

The invention discloses a stem cell culture medium. The stem cell culture medium comprises an improved DMEM (Dulbecco's Modified Eagle Medium) / F12 basal culture medium and an additive, wherein the additive comprises sodium bicarbonate, selenium amino acid chelate, recombinant human insulin growth factors, recombinant human basic fibroblast growth factors, recombinant human lactoferrin, ascorbic acid, recombinant human platelet-derived growth factors, recombinant human vascular endothelial cell growth factors, octacosanol, polyvinyl alcohol, polyvinylpyrrolidone and recombinant human epidermal growth factors. According to the stem cell culture medium, the potential of stem cells is not influenced while the stem cells can be proliferated rapidly, the proliferation speed of the stem cells is increased by 3-5 times compared with a common culture medium, and further, the stem cell culture medium can be used for culturing the stem cells of various kinds of tissue and has excellent applicability; the cultured stem cells have high differentiation capability, can be differentiated into multiple functional cells and have very high scientific research and medical application values, culture medium components are exact, the quality is stable, and accordingly, the cultured stem cells are not likely to generate human body rejection reaction after transplanting.

Description

technical field [0001] The invention relates to the technical field of stem cell culture, in particular to a stem cell culture medium. Background technique [0002] Many studies have shown that stem cells can not only self-renew, but also differentiate into other functional cells under suitable conditions. Therefore, stem cells are expected to become an effective means of treating difficult human diseases. However, the content of stem cells in normal adult tissues is very small. How to rapidly expand and cultivate stem cells in vitro is an important technology for studying the mechanism of stem cells and exploring their therapeutic methods in the treatment of human diseases. [0003] The content of stem cells is as small as possible, but they are widely distributed in various tissues and organs of mammals, including but not limited to bone marrow, umbilical cord, adipose tissue, brain tissue, retina, heart, liver, lung and skin. A large number of studies have shown that the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0735C12N5/074C12N5/0775C12N5/0789C12N5/0797C12N5/095
Inventor 李晓娟白瑞樱郭勇王歌郭直岳朱晓谦王亚莉
Owner XINXIANG MEDICAL UNIV
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