Method and application of hyaluronic acid promoting proliferation of human amniotic membrane stem cells
A technology of hyaluronic acid and stem cells, which is applied in non-embryonic pluripotent stem cells, artificially induced pluripotent cells, animal cells, etc., can solve the problems of high price, high in vitro expansion of hASCs, and limitations.
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Embodiment 1
[0078] Embodiment 1 of the present invention: a method for promoting the proliferation of human amniotic membrane stem cells by hyaluronic acid, collecting the cultured second-generation human amniotic membrane epithelial cells, seeding them in 96-well plates with 3000-5000 cells / well, and replacing them after 24 hours L-DMEM full medium; then add hyaluronic acid to the culture medium to make the concentration of hyaluronic acid reach 0.01-20 mg / mL; place the culture plate in saturated humidity, 5% CO 2 In an incubator, culture at 37° C. for 12-168 h and stop; the molecular weight of the hyaluronic acid is 20-2200 kDa.
Embodiment 2
[0079] Embodiment 2 of the present invention: the application of hyaluronic acid in the preparation of a drug for promoting the proliferation of human amniotic membrane stem cells. Hyaluronic acid can be used alone, or can be combined with cell growth factors to make powder or water according to conventional methods.
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