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Sensor for detecting bean protein A and preparation method and application thereof

A technology of concanavalin and sensors, applied in instruments, measuring devices, scientific instruments, etc., can solve problems such as result distortion, and achieve the effects of convenient detection, strong operability, and simple process

Active Publication Date: 2018-12-28
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These single-signal detection methods are susceptible to distorted results due to changes in the electrode surface and dissociation of reagents induced by non-target substances, especially in complex biological environments such as cells and body fluids

Method used

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  • Sensor for detecting bean protein A and preparation method and application thereof
  • Sensor for detecting bean protein A and preparation method and application thereof
  • Sensor for detecting bean protein A and preparation method and application thereof

Examples

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preparation example Construction

[0090] The present invention also provides a method for preparing the sensor for detecting concanavalin A, comprising the following steps:

[0091] The G-CdTe QDs are combined with the recognition unit through π-π stacking, and are modified on the surface of the pretreated electrode; the anode luminescent body combined with the recognition unit is then modified and incubated to obtain the sensor.

[0092] In a preferred embodiment of the present invention, the G-CdTe QDs are dispersed in water, and the dispersion concentration is 0.5-2.0 mg / mL.

[0093]In a preferred embodiment of the present invention, the identification unit includes Dexp. Preferably, the concentration of Dexp is 5-30 mg / mL. More preferably, the Dexp is made into an aqueous solution of Dexp.

[0094] In a preferred embodiment of the present invention, the volume ratio of the G-CdTe QDs dispersion to the Dexp solution is 1:(0.8-1.2), preferably 1:1.

[0095] In a preferred embodiment of the present inventi...

Embodiment 1

[0138] This embodiment provides a method for preparing a sensor for detecting concanavalin A, the steps are as follows:

[0139] 1. Preparation of G-CdTe QDs

[0140] Weigh 36.89mg of CdCl 2 2.5H 2 O was dissolved in 50 mL of deionized water, and 220 μL of 1 mg / mL GO was added to the above solution under stirring conditions and continued to stir for 1 h. Then gradually add 1 mL of 0.01 M Na 2 TeO 3 , 50mg of C 6 h 5 Na 3 o 7 2H 2 O, 33 μL of MPA and 100 mg of NaBH4 And continue to stir, after heating and reflux at 130°C for 10h, wash and centrifuge three times with ethanol and distilled water with a volume ratio of 1:1 to collect G-CdTe QDs, disperse in deionized water and store at 4°C for later use. 1 mg / mL.

[0141] 2. Preparation of anode emitter Dexp-ABEI-Au-Pt

[0142] 1 mL of 1% HAuCl 4 The solution was added dropwise to 2 mL of 10 mmol / L ABEI solution under stirring condition, and stirred and reacted at room temperature for 2 h. Then 1 mL of 1% H 2 PtCl 6...

Embodiment 2

[0147] This embodiment provides a method for preparing a sensor for detecting concanavalin A, the steps are as follows:

[0148] 1. Preparation of G-CdTe QDs

[0149] Same as Example 1

[0150] 2. Preparation of anode emitter Dexp-ABEI-Au-Pt

[0151] 1 mL of 1% HAuCl 4 The solution was added dropwise to 2 mL of 10 mmol / L ABEI solution under stirring condition, and stirred and reacted at room temperature for 2 h. Then 1 mL of 1% H 2 PtCl 6 The solution was added dropwise to the above mixed solution with stirring and continued to stir for 2h. Then the above mixed solution was centrifuged at 10000rpm for 15min, and washed three times with deionized water, and the obtained anode emitter ABEI-Au-Pt was dispersed into double distilled water, the dispersion concentration was 1.5mg / mL, and 1mL was added, and the concentration It was a 15 mg / mL Dexp solution, stirred for 2 hours to obtain the anode emitter Dexp-ABEI-Au-Pt combined with the recognition unit, and stored at 4°C for ...

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Abstract

The invention relates to the technical field of electrochemiluminescence sensors, in particular to a sensor for detecting bean protein A and a preparation method and application thereof. The sensor for detecting bean protein A comprises a cathode emitter, an anode emitter and a co-reagent. The cathode emitter comprises graphene oxide modified cadmium telluride quantum dots, the anode emitter comprises nanogold and nano-platinum modified N-(aminobutyl)-N-(ethylisoluminol). The preparation method comprises the following steps: combining graphene oxide modified cadmium telluride quantum dots anda recognition unit by pi-pi stacking, and modifying the surface of the pretreated electrode; modifying the anode emitter combined with the recognition unit and incubating to obtain the sensor. According to the sensor for detecting bean protein A and preparation method and application thereof, the ratio type electrochemiluminescence system is constructed on the basis of competitive consumption of oxygen, so that the signal to noise ratio is improved, and the detection result is high in reliability.

Description

technical field [0001] The invention relates to the technical field of electrochemiluminescence sensors, in particular to a sensor for detecting concanavalin A, its preparation method and application. Background technique [0002] Electrogenerated Chemiluminescence (ECL) is a new type of detection technology combining electrochemical and spectroscopic methods. It has the advantages of strong versatility, simple optical device, and good time and space controllability. , Molecular recognition, DNA detection and immune analysis have been widely used. [0003] At present, most ECL detection is based on single-signal detection. These single-signal detection methods are susceptible to distorted results due to changes in the electrode surface and dissociation of reagents induced by non-target substances, especially in complex biological environments such as cells and body fluids. Therefore, overcoming the interference of environmental factors and improving the sensitivity and rel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327G01N27/38
CPCG01N27/26G01N27/30G01N27/305G01N27/3278G01N27/38
Inventor 陈时洪张涵谭兴容
Owner SOUTHWEST UNIVERSITY
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