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Application of astraisoflavan-7-O-beta-D-glucoside in preparation of immunity inhibitors and vasodilators

An immunosuppressant and vasodilation technology, applied in the field of medicine, can solve the problems of unreported use

Inactive Publication Date: 2014-10-22
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Astragalus isoflavane is one of the important compounds in astragalus isoflavones, and its use in immunosuppression and vasodilation has not been reported yet

Method used

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  • Application of astraisoflavan-7-O-beta-D-glucoside in preparation of immunity inhibitors and vasodilators
  • Application of astraisoflavan-7-O-beta-D-glucoside in preparation of immunity inhibitors and vasodilators
  • Application of astraisoflavan-7-O-beta-D-glucoside in preparation of immunity inhibitors and vasodilators

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: Effect of astragalus isoflavanoids on the proliferation of mouse T cells induced by concanavalin A (ConA)

[0011] 1 material

[0012] 1.1 Animals

[0013] BALB / c mice, male, weighing 18-20 g, were provided by the Experimental Animal Department of Peking University (permit number: SCXK11-00-0008). The animals were kept in a standard environment: temperature (23±1)°C, relative humidity (55±5)%, light and dark cycle 12h / 12h, and standard pellet feed.

[0014] 1.2 Reagents

[0015] Concanavalin A (Con A) and thiazolyl blue (MTT) were purchased from Sigma Company of the United States; fetal bovine serum (FBS) was purchased from GIBCO Company of the United States; red blood cell lysate, 1640 culture medium, and phosphate (PBS) buffer were purchased from From Beijing Maichen Biotechnology Company; DMSO (dimethyl sulfoxide, batch number 20101008) was purchased from Beijing Chemical Plant. Astragalus isoflavanoid samples were prepared in DMSO.

[0016] 1.3 Instr...

experiment example 2

[0028] Experimental Example 2: Effect of Actascoisoflavone on Potassium Chloride (KCl)-Induced Rat Vascular Ring Contraction

[0029] 1 material

[0030] 1.1 Animals

[0031] SD rats, weighing 180-200 g, half male and half male, were provided by the Experimental Animal Department of Peking University (permit number: SCXK11-00-0008). The animals were kept in a standard environment: temperature (23±1)°C, relative humidity (55±5)%, light and dark cycle 12h / 12h, and standard pellet feed.

[0032] 1.2 Reagents

[0033] Gram-Heinz (K-H) solution preparation: NaCl118.0, KCl5.4, NaH 2 PO 3 41.0, MgSO 4 ·7H 2 O1.2, CaCl 2 1.9, NaHCO 3 25.0 and glucose 11.1 (mmol·L -1 ), adjust the pH to be 7.4. Water-soluble samples were prepared in PBS, and fat-soluble samples were prepared in DMSO.

[0034] 1.3 Instruments

[0035] Constant temperature water bath, tension transducer, biological collection system, etc.

[0036] 2 methods

[0037] Stun the rats, quickly take the thoracic ...

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PUM

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Abstract

Through the researches and experiments on astraisoflavan-7-O-beta-D-glucoside, it has been found for the first time that astraisoflavan-7-O-beta-D-glucoside can prominently inhibit T lymphocyte proliferation induced by concanavalin A (Con A) and rat isolated arterial ring contracts induced by potassium chloride (KCl), has immunity inhibiting and vasodilative effects, and is advantageously used to prepare immunity inhibitors and vasodilators.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the use of astragalus isoflavanoids in the preparation of immunosuppressants and vasodilation drugs. Background technique [0002] Astragalus membranaceus is a traditional Chinese medicine that strengthens the spleen and replenishes qi. It has the functions of invigorating qi and solidifying the surface, diuretic and detoxification, draining pus, astringing sores and promoting granulation. Hematochezia and metrorrhagia, surface deficiency and spontaneous sweating, Qi deficiency and edema, carbuncle and gangrene, persistent ulceration, blood deficiency and yellowish yellow, internal heat and thirst, chronic nephritis, proteinuria and diabetes (see: National Pharmacopoeia Committee. Pharmacopoeia of the People's Republic of China ( Part One) [z]. Beijing: Chemical Industry Press, 2005: 212-213.). Modern pharmacological studies believe that Astragalus can regulate the immune function of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P37/06A61P9/08
Inventor 徐风叶加张微微蔡少青
Owner PEKING UNIV
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