87results about How to "No need to mark" patented technology

The present invention provides a protein micro-array surface plasma resonance imaging detection system and its detection method. Said system includes surface plasma resonance micro-array protein sensor, incident arm, reflecting arm and signal processing unit, the described sensor includes prism, micro-array chip and sample cell, between prism and micro-array chip a refractivity oil layer is coated, the incident arm is positioned at one side of the sensor, successively includes semiconductor laser, collimator, polarizer, attenuator and reactangular light diaphragm, the reflecting arm is positioned at another side of the sensor, and successively includes lens and CCD receiver, and signal processing unit includes signal processing circuit and computer.

The invention discloses a multi-RCA (rolling circle amplification) method based on split padlock probes. Each novel split padlock probe is about 90bp in length and comprises four parts, namely a detection arm, a universal primer area, an HhaI endonuclease site and a tag sequence area, wherein an amplification system is composed of a connection system and an RCA system. The concrete detection method comprises the steps of: firstly, carrying out coupled reaction, mixing a target sequence DNA (deoxyribonucleic acid) segment and four split padlock probes of which the final concentration is 1mol/L, hybridizing for 15 minutes after boiling and degenerating, adding T4DNA ligase and T4DNA ligase buffer solution, supplying 10 muL of reaction system; carrying out exonuclease I and exonuclease III exterior contact for 45 minutes at 37 DEG C, preparing an annular template and then carrying out RCA reaction; mixing, boiling and degenerating 10 muL of connection product and universal primer, respectively adding dNTP, phi29DNA polymerase, HhaI restriction enzyme and buffer solution to form 20 muL of reaction system, stewing for 60 minutes at 37 DEG C; finally detecting single-chain DNA product of RCA with a specific tag sequence, thus obtaining a corresponding test conclusion according to the result.

The invention discloses a method for detecting fungaltoxin through multiple signals. The method comprises the following steps: 1) combining fungaltoxin with aptamer: adding a to-be-detected sample after the reaction of the fungaltoxin aptamer and complementary sequence thereof, thereby acquiring a mixed solution A; 2) amplifying a digestion auxiliary signal: reacting the mixed solution A with restriction enzyme, thereby acquiring a mixed solution B; 3) preparing a guanine tetramer structure: reacting the mixed solution B with tail-end deoxynucleotide transferase and deoxyribonucleoside triphosphate and reacting with ligand molecules, thereby acquiring a mixed solution C; and 4) detecting and analyzing: performing catalytic oxidation reaction on the mixed solution C and different substrates, generating ultraviolet, fluorescent and chemiluminescent signals and calculating the content of fungaltoxin in the to-be-detected sample according to the relation of the response strength of all the signals and the fungaltoxin concentration. The method disclosed by the invention has the characteristics of quick and simple operation and treatment, short detection time, marker-free effect, low cost, high precision, high sensitivity, and the like.

The invention relates to a method for determining surfactant critical micelle concentration (CMC) based on the terahertz time-domain spectroscopy technology. The method comprises the steps that 1, a sample pool suitable for terahertz spectrum detection is prepared; 2, surfactant solution samples with different concentrations crossing four orders of magnitudes from 0.1 mM to 100 mM are prepared; 3, terahertz time-domain spectrum of a sample solution to be detected is measured and obtained, a relation spectrogram between the concentrations of the samples and terahertz absorption coefficients or refractive indexes are calculated and obtained through a formula, and the concentration, corresponding to a turning point of the spectrogram, of the surfactant sample is determined; 4, surfactant water solution samples which have a series of concentrations with smaller intervals are prepared according to the concentrations selected close to the determined sample turning point concentration; 5, terahertz time-domain spectra of sample solutions to be detected are measured and obtained again; 6, relation spectrograms between the concentrations of the samples to be detected and terahertz absorption coefficients or refractive indexes are calculated and obtained again, and the CMC of a surfactant is determined through spectrogram information. The method has the advantages of being rapid, free of marks, easy to operate, wide in application range, good in repeatability, high in result accuracy and the like.

Provided are a thyroid gland neck tissue classifying and identifying system and method based on an autofluorescence technology and an optical coherence tomography. The system organically integrates anautofluorescence system and an optical coherence tomography system, utilizes high-sensitivity tissue element resolution capability of the autofluorescence system and high-resolution tissue structureimaging of the optical coherence tomography system to achieve quick, identifier-free, nondestructive, high-sensitivity and real-time thyroid gland neck tissue classification and identification. By adopting the thyroid gland neck tissue classifying and identifying system and method, the classification and identification efficiency and accuracy of doctors to parathyroid glands, thyroid glands, fat,lymph glands and other neck tissues in clinic thyroid gland operation can be effectively improved, the doctors can be assisted to protect the parathyroid glands and thoroughly remove the lymph glands.

The invention discloses a composite nano-probe based on N-Ti<3>C<2>QDs and o-phenylenediamine oxide, and discloses a ratio fluorescence detection method of the probe for H<2>O<2> and xanthine. The probe is the composite nano-probe, which is formed by bonding N-Ti<3>C<2>QDs to DAP through pi-pi conjugation and hydrogen bonds and used for ratio fluorescence detection of H<2>O<2> or xanthine. According to the invention, a dual-emission reverse ratio fluorescent sensing platform of H<2>O<2> based on photo-induced electron transfer (PET) is established by using quantum dots synthesized by a MXene two-dimensional material for the first time; furthermore, on the basis of the effect of xanthine generating H<2>O<2> under specific catalysis of xanthine oxidase, ratio fluorescence detection of xanthine is realized; and the detection method in the invention has the advantages of being free from labelling, high in sensitivity, high in accuracy and low in cost detection, and has good application prospect.

The invention discloses a fluorescence chemical method for detecting ALP (Alkaline Phosphatase) and application thereof. The fluorescence chemical method comprises the following steps of: firstly, designing a single-chain DNA (Deoxyribonucleic Acid) of which a 3' terminal is phosphorylated as a DNA probe, dephosphorylating the 3' terminal of the DNA probe and exposing a 3'-OH terminal when the ALPexists; secondly, performing catalytic addition on dTTPs (Deoxythymidine Triphosphates) at a 3'-OH terminal of the DNA probe by TdT (Terminal Deoxynucleotidyl Transferase), thus extending to generatea poly-T tail ssDNA, forming a dual-chain DNA mediated by T-HgII-T structure by the poly-T tail ssDNA and Hg ions, and drawing a detecting curve according to a detected fluorescence intensity signaland the concentration of the ALP after adding nucleic acid dye; finally, replacing the ALP with a to-be-detected sample, repeating the steps, and comparing a detecting result with the detecting curve,thus calculating the content of the ALP in the sample. The fluorescence chemical method disclosed by the invention is simple and convenient, is high in sensitivity and can be used for detecting the ALP or screening ALP inhibitors.

The invention relates to a rapid detection method for metal ions in wine. The method comprises the following steps: (1) a Schiff base metal ion probe is prepared by a Schiff base reaction; (2) gold nanorods (Au) are synthesized through seed growth; (3) the metal irons and the Schiff base are subjected to complexing reaction, and a Schiff base metal complex is formed; and (4) the generated complexis detected by using a portable Raman spectrometer, an ultraviolet spectrophotometer and a fluorescence spectrophotometer, and thus, qualitative and quantitative analysis and detection on Cu<2+> and Zn<2+> contained in a wine product are thus carried out. In comparison with the prior art, the Schiff base metal complex is detected by using SERS, ultraviolet and fluorescence sensors, the detection is more accurate, labeling and separation and purification do not needed, monitoring and detection on Cu<2+> and Zn<2+> in a wine product during the production and storage process of the wine can be realized, and the detection limit is 1*10<-10> M.

The invention discloses a sensor with a gold nanoparticle and quantum dot composite structure, a system and a method. Based on the local surface plasma resonance characteristics of the gold nanoparticles under total internal reflection, the microfluid technology is combined, the gold nanoparticles are directly combined with the transparent substrate, the binding force is strong, the thickness is accurate and controllable, the continuity is good, no pollution is caused, in addition, the optical property of the gold film is excellent, and the sensitivity and the accuracy are very good. Quantum dots are inserted into gaps among gold nanoparticles to obtain a gold nanoparticle quantum dot composite structure, the surface plasma resonance effect of the gold nanoparticles is enhanced, the sensoris more sensitive to changes of parameters such as the refractive index and the thickness of the composite structure and media nearby the composite structure, and research substances attached to thesurface of the structure can be detected more easily. The sensor has the advantages of high sensitivity, high stability, no marking, no damage, high accuracy, real-time rapid detection, wide application range and the like; the sensor has a great development prospect and a wide potential application value.

The invention discloses a polymer film conformation transition temperature detection method and system. The method comprises the following steps: measuring a terahertz time-domain spectral signal of atemperature control sample pool; selecting a temperature point according to a first preset interval in the temperature rise range of the temperature control sample pool to measure a first terahertz time-domain spectral signal of the thin film sample; determining the turning temperature of the film sample according to the terahertz time-domain spectral signal of the temperature control sample pooland the first terahertz time-domain spectral signal; selecting a temperature point according to a second preset interval within a preset temperature range of the transition temperature to measure a second terahertz time-domain spectral signal of the thin film sample; and determining the conformational transformation temperature of the film sample according to the terahertz time-domain spectral signal of the temperature control sample cell and the second terahertz time-domain spectral signal. The terahertz time-domain spectroscopy technology is used for detecting the conformational transformation temperature of the polymer film, so that the method has the advantages of being rapid, free of marks, easy to operate, wide in application range, good in repeatability, high in result accuracy andthe like.

The invention relates to a DNA sensor based on a 3 (2, 2'-bipyridyls) ruthenium solid electrochemiluminescence, a preparation method and applications used for the DNA sensor, belonging to the technical field of analytical chemistry and chemical sensors. The DNA electrochemiluminescence sensor is prepared by the modified glass carbon electrode of a nanometer pipe/a perfluorosulfonic acid ion-exchange membrane/3(2, 2'-bipyridyls) ruthenium and the catalytic oxidation reaction of guanine base and adenine base conducted on the basis of 3(2, 2'-bipyridyls) ruthenium; the DNA sensor can be used for the electrochemistry and electrochemiluminescence double detection of the DNA, and can be also used for the electrochemistry and electrochemiluminescence double detection of thermally denaturated DNA; and furthermore, the DNA sensor can be also used for the electrochemistry and electrochemiluminescence detection of DNA single base mismatch. The DNA sensor has high sensitiveness and simple operation, and needs no marks during the detection process; and the detection of single base mismatch has universality and can be used for the detection of DNA fragments with any sequences.

The invention relates to a preparation method of a graphene biosensor for specific protein detection. Graphene in the specific protein biosensor is prepared by a high-temperature annealing method in an inert atmosphere. A functional graphene film is designed and prepared, and the graphene specific protein biosensor is also manufactured. Tests show that the specific protein biosensor can perform real-time unmarked detection on specific protein and allows influences caused by concentration changes of target protein to be seen clearly. A graphene biochip has a bright application prospect in the aspects of biomolecule sensors and biomolecule detection.

The invention discloses a three-dimensional culture human myocardial cell pulsation detection method based on image edge extraction. The three-dimensional culture human myocardial cell pulsation detection method specifically comprises the following steps: taking a first frame of a myocardial cell pulsation video sequence frame as a reference image; converting the reference image into a grayscale image; obtaining a sub-image of each myocardial cell cluster; extracting coordinates and gray values of edge position points of each myocardial cell group, and calculating a gray average value of reference edge points of each myocardial cell group; taking a sequence frame of a subsequently acquired myocardial cell pulsation video as a real-time image, and carrying out parallel graying processing; according to the coordinate positions of the reference edge points in the reference image, calculating a gray average value of the reference edge points of each myocardial cell group in the real-time image; drawing a myocardial cell pulsation curve of each myocardial cell cluster; and detecting the cardiac muscle cell pulsation frequency and amplitude in the cardiac muscle cell pulsation curve. Thecardiac muscle cell pulsation characteristics are automatically detected based on the image edge extraction technology, and the three-dimensional culture human myocardial cell pulsation detection method has the advantages of being intelligent, non-invasive, low in cost, repeatable and real-time in processing.

The invention discloses a difunctional G-quadruplex allosteric biosensor for detecting beta-lactoglobulin. The difunctional G-quadruplex allosteric biosensor is prepared by the following steps: (1) designing a difunctional G-quadruplex aptamer of beta-lactoglobulin; (2) optimizing the reaction conditions of the beta-lactoglobulin biosensor; and (3) detecting the beta-lactoglobulin. According to the invention, the design principle is that the beta-lactoglobulin biosensor sequence is subjected to conformational change before and after a detection target is introduced, the catalase activity of G-quadruplex/hemin DNA bionic enzyme is influenced, and then beta-lactoglobulin detection is realized; and the G-quadruplex sequence responds to the target concentration, such that the solution presents the corresponding colorimetric gradient after 3, 3', 5, 5'-tetramethyl benzidine (TMB) color development is catalyzed so as to finally achieve the simple, rapid, low-cost and ultra-sensitive colorimetric detection of the beta-lactoglobulin.

The invention discloses an anti-AChE interference method capable of quickly detecting BChE activity. The method comprises the following steps of: 1) with sulfonated calix[4]arene and dye lucigenin as a sensing pair for enzyme activity detection and succinylcholine as an enzyme reaction substrate, preparing a solution containing 0.5(mu)mol/L SC4A, 0.5(mu)mol/L LCG and 10(mu)mol/L SuCh by use of 10mmol/L phosphoric acid buffer solution with pH being 8.0 at 37 DEG C, and establishing a supermolecule serial detection system; and 2) adding 1U/ml BChE into the system to obtain an obvious fluorescence quenching signal within 15 minutes. The method disclosed by the invention has the advantages of simple technology, convenience in operation and sensitivity and high speed, can detect the activity of BChE with high selectivity in the presence of excessive AChE, and has broad application prospect in the specific detection of BChE activity and treatment field of Alzheimer's disease.