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Composite nano-probe based on N-Ti<3>C<2>QDs and o-phenylenediamine oxide, and ratio fluorescence detection method thereof

An o-phenylenediamine and nano-probe technology is applied in the field of analysis and detection, which can solve the problems of inaccurate number of detection probes, micro-environmental interference, high system error, etc., and achieve the effect of improving the fluorescence quantum yield.

Active Publication Date: 2020-01-24
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most fluorescent sensors are detected based on emission data of a single wavelength, which is susceptible to fluctuations in excitation sources, inaccurate detection probe numbers, and interference from microenvironments.
These instabilities can lead to high systematic errors leading to inaccurate and non-reproducible results

Method used

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  • Composite nano-probe based on N-Ti&lt;3&gt;C&lt;2&gt;QDs and o-phenylenediamine oxide, and ratio fluorescence detection method thereof
  • Composite nano-probe based on N-Ti&lt;3&gt;C&lt;2&gt;QDs and o-phenylenediamine oxide, and ratio fluorescence detection method thereof
  • Composite nano-probe based on N-Ti&lt;3&gt;C&lt;2&gt;QDs and o-phenylenediamine oxide, and ratio fluorescence detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A N-Ti based 3 C 2 Composite nanoprobes of QDs and o-phenylenediamine oxide for H 2 o 2 A ratiometric fluorescence detection method comprising the steps of:

[0048] (1)N-Ti 3 C 2 Synthesis of QDs: 15mg Ti 3 C 2 MXene was dissolved in 15mL DMF, N 2 Ultrasonic deoxygenation under protection for 30min, NH 3 ·H 2 O to adjust the pH to 9.0, followed by N 2 Under protection, it was quickly transferred to a reaction kettle for reaction at 120°C for 6 hours, cooled to room temperature and filtered through a 0.22 μm microporous membrane.

[0049] N-Ti 3 C 2 For the characterization of QDs see figure 1 and figure 2 .

[0050] figure 1 A and B are respectively N-Ti 3 C 2 TEM and size distribution of QDs, calculated and analyzed by ImageJ, N-Ti 3 C 2 QDs are evenly distributed, with an average particle size of 3.4±0.5nm; figure 1 The inset in A is the HRTEM image, which further reveals the N-Ti 3 C 2 Crystal characteristics of QDs. figure 1 C, D are the fu...

Embodiment 2

[0056] A N-Ti based 3 C 2 The compound nanoprobe of QDs and o-phenylenediamine oxide is used for the ratio fluorescence detection method of xanthine, comprises the following steps:

[0057] (1)N-Ti 3 C 2 Synthesis of QDs: 15mg Ti 3 C 2 MXene was dissolved in 15mL DMF, N 2 Ultrasonic deoxygenation under protection for 30min, NH 3 ·H 2 O to adjust the pH to 9.0, followed by N 2 Under protection, it was quickly transferred to a reaction kettle for reaction at 120°C for 6 hours, cooled to room temperature and filtered through a 0.22 μm microporous membrane.

[0058] (2)N-Ti 3 C 2 Preparation of QDs@DAP probe: Mix 150 μL of xanthine solutions with concentrations of 1 μM, 10 μM, 25 μM, 40 μM, and 50 μM with 190 μL of o-phenylenediamine at a concentration of 40 mM and 10 μL of xanthine oxidase at a concentration of 100 μg / mL , 10 μL of horseradish peroxidase with a concentration of 8 U / mL and 200 μL of PB buffer solution were mixed and incubated at 37° C. in the dark for 5...

Embodiment 3

[0062] Selective experiment of detection method of the present invention

[0063] 1) Detection of H 2 o 2 The selectivity: repeat step 2) and step 3) among the embodiment 1), the H wherein 2 o 2 Replaced with dopamine, ascorbic acid, phenylalanine, glucose, lysine, L-cysteine, tyrosine, glutathione, oxidized glutathione, K + ,Mg 2+ , Ca 2+ , Na + ,Zn 2+ ,Cd 2+ , Fe 2+ , Ag + ,I - and Br - Interfering substances, other conditions remain unchanged, detection of fluorescence, get this method to detect H 2 o 2 The selectivity result graph (see Figure 5 ).

[0064] Figure 5 Among them, DA, AA, Phe, Glu, Lys, L-Cys, Tyr, GSH, GSSG are respectively dopamine, ascorbic acid, phenylalanine, glucose, lysine, L-cysteine, tyrosine, gluten Glutathione, oxidized glutathione, it can be seen from the figure that this method has good selectivity to the target H2O2.

[0065] 2) Selectivity of xanthine detection: repeat step 2) and step 3) in Example 2, replace xanthine therei...

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Abstract

The invention discloses a composite nano-probe based on N-Ti<3>C<2>QDs and o-phenylenediamine oxide, and discloses a ratio fluorescence detection method of the probe for H<2>O<2> and xanthine. The probe is the composite nano-probe, which is formed by bonding N-Ti<3>C<2>QDs to DAP through pi-pi conjugation and hydrogen bonds and used for ratio fluorescence detection of H<2>O<2> or xanthine. According to the invention, a dual-emission reverse ratio fluorescent sensing platform of H<2>O<2> based on photo-induced electron transfer (PET) is established by using quantum dots synthesized by a MXene two-dimensional material for the first time; furthermore, on the basis of the effect of xanthine generating H<2>O<2> under specific catalysis of xanthine oxidase, ratio fluorescence detection of xanthine is realized; and the detection method in the invention has the advantages of being free from labelling, high in sensitivity, high in accuracy and low in cost detection, and has good application prospect.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, in particular to 3 C 2 Composite nanoprobes of QDs and o-phenylenediamine oxides and their ratiometric fluorescence detection method, especially for H 2 o 2 or a ratiometric fluorescence detection method for xanthines. Background technique [0002] During life, hydrogen peroxide (H 2 o 2 ) are produced by aerobic respiration and serve as messengers in signaling pathways. overproduced H 2 o 2 The accumulation of oxidative stress and corresponding pathological changes are closely related to Parkinson's disease and Alzheimer's disease. Therefore, H 2 o 2 can be used as a potential diagnostic marker. However, since H 2 o 2 At lower concentrations, its exact role in these neuronal dysfunctions and its relationship to other diseases has not been elucidated. [0003] In addition, as a general product of oxidases, H 2 o 2 It can be used as a general signaling molecule to de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C09K11/67C09K11/06C01B32/97B82Y30/00
CPCG01N21/6428C09K11/67C09K11/0883C09K11/06C01B32/97B82Y30/00G01N2021/6432
Inventor 周学敏朱婉莹卢巧云王晶李昺之翁晨园李晓芸杨威严孝强
Owner NANJING MEDICAL UNIV
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