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47results about How to "Low cost detection" patented technology
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Comprehensive ocular surface analyzer based on expert system
InactiveCN104398234AImprove objectivityGood repeatabilityEye diagnosticsImaging processingComputer science
The invention relates to a comprehensive ocular surface analyzer based on an expert system. The comprehensive ocular surface analyzer comprises a comprehensive ocular surface analyzer hardware module, an image processing module and an expert system module, wherein the comprehensive ocular surface analyzer hardware module comprises a controller, a structured illumination module and an imaging module; the image processing module comprises a tear meniscus height measuring module, a red eye detection module, a tear film stability detection module, a meibomian gland morphology detection module and a corneal topography analysis module; and the expert system takes information acquired though image processing as the input, performs objective and correct judgment on common diseases of the human ocular surface and provides therapeutic measures.
Owner:XIAMEN UNIV
In-Vehicle Image Processing Device and Method
InactiveUS20150035984A1Low cost detectionLow costTelevision system detailsRoad vehicles traffic controlParallaxCMOS sensor
The object of the present invention is to improve the detection capability for detecting a preceding vehicle that may collide with a driver's vehicle using a stereo camera having rolling shutter types of CMOS sensor. The present invention relates to an in-vehicle image processing device that includes: plural imaging sections for imaging the area ahead of a driver's vehicle; an image processing section for detecting another vehicle using disparity information about plural images obtained by the imaging sections. In this case, the imaging sections include imaging devices the exposure timing of each of which is different on the basis of a line of the imaging screen, and the imaging devices are sequentially exposed in the direction from the lowermost edge to the uppermost edge of the another vehicle.
Owner:HITACHI AUTOMOTIVE SYST LTD
Method for detecting toxic impurity DMAP in antibiotic drug raw materials
ActiveCN105891348AMeet the test requirementsEasy to operateComponent separationRetention timeQuadrupole
The invention discloses a method for detecting the toxic impurity DMAP in antibiotic drug raw materials. The antibiotic drug raw materials are dissolved, diluted and centrifuged to obtain supernate, the supernate is subjected to high performance liquid chromatography-quadrupole series linear ion trap mass spectrum, and then the content of the toxic impurity DMAP in the antibiotic drug raw materials is detected. The mobile phase of high performance liquid chromatography is a mixture of ammonium acetate aqueous solution and acetonitrile solution. The mass-to-charge ratio (m / z) of the parent ion in quadrupole series linear ion trap mass spectrum for detecting DMAP is 122.7, and the mass-to-charge ratio (m / z) of the daughter ion is 107.1. The adopted pair of ions does not interfere with sample detection and is highest in response. By the adoption of the mobile phase, the retention time of a target compound on a chromatographic column can be prolonged, so that a detection result is more stable. The method is easy to operate, and the result is reliable. Quick and low-cost detection of the residual quantity of DMAP in the antibiotic drug raw materials can be achieved, and detection sensitivity can reach 0.50 mg / kg.
Owner:SHANDONG ANALYSIS & TEST CENT
Method for synchronously detecting biochemical oxygen demand and nitrate nitrogen content in water body based on bidirectional electron transfer electrochemical active microorganisms
ActiveCN111175356ANo cumbersome replacementLow cost detectionMaterial electrochemical variablesMicroorganismWater quality
The invention relates to a method for synchronously detecting biochemical oxygen demand and nitrate nitrogen content in a water body based on bidirectional electron transfer electrochemical active microorganisms and mainly aims to solve problems of detection hysteresis, complex operation, high cost and incapability of synchronous detection in the water body water quality biochemical oxygen demandand nitrate nitrogen detection process in the prior art. The method is advantaged in that a bioelectrochemical system taking electrochemical active microorganisms with bidirectional electron transfercapability as a core is constructed, step potentials are set to measure the electricity generation capacity and the electrophilic capacity of the system respectively, and rapid real-time detection ofbiochemical oxygen demand and nitrate nitrogen in the water body can be realized by utilizing an output current signal during electricity generation of the system, an input current signal during electrophilic and a standard curve model of biochemical oxygen demand and nitrate nitrogen.
Owner:BEIHANG UNIV
Rolling circle constant-temperature amplification quick detection primer and kit for mycoplasma pneumoniae
ActiveCN106399542AGreat potentialHigh sensitivityMicrobiological testing/measurementMicroorganism based processesMicroorganismMycoplasma pneumonia
The invention belongs to the field of microbiological detection, and particularly relates to a rolling circle constant-temperature amplification quick detection primer and a kit for mycoplasma pneumonia. The primer is designed according to a specific conservative target sequence of a mycoplasma pneumonia P1 gene; the primer consists of four primers, including a front end inner primer SIP, a rear end inner primer ASIP, and outer primers S and AS; in the pair of the outer primers S and AS, the S primer is positioned on the upstream of the SIP, and the AS primer is positioned on the downstream of the ASIP. The rolling circle constant-temperature amplification quick detection primer and the kit for the mycoplasma pneumonia can be used for quick detection of the mycoplasma pneumonia, provide guarantee for clinical early diagnosis and treatment, and realize an important value of quick, accurate, convenient and low-cost detection.
Owner:沈阳优宁生物科技有限公司
Traffic light control system and method
InactiveCN112349113ALess destructiveLow cost detectionControlling traffic signalsDetection of traffic movementInformation controlTelecommunications
The invention discloses a traffic light control system, and relates to the field of traffic light control. The system comprises a geomagnetic detector module, a traffic light module and a control host. The control host is connected with the geomagnetic detector module and the traffic light module; the geomagnetic detector module is used for detecting vehicle information at an intersection; the geomagnetic detector module comprises a first geomagnetic detector and a second geomagnetic detector; the first geomagnetic detector is arranged at a first detection point of an entrance of the intersection, the second geomagnetic detector is arranged at a second detection point of the entrance of the intersection, and the distance between the first detection point and the intersection is smaller than the distance between the second detection point and the intersection; and the control host is used for judging the traffic jam condition of the intersection according to the vehicle information detected by the geomagnetic detector module and controlling the work of the traffic light module. According to the system and a method provided by the invention, the low-cost detection of the vehicle information is realized through the geomagnetic detector, and then the traffic lights are controlled through the vehicle information so as to relieve the traffic jam condition.
Owner:TAIZHOU POLYTECHNIC COLLEGE
Method capable of quickly detecting paraquat in beverages
InactiveCN106814060AQuick QualificationQuick checkPreparing sample for investigationRaman scatteringFruit juiceNanoparticle
The invention belongs to the field of pesticide detection and provides a method capable of quickly detecting paraquat in beverages. The method capable of quickly detecting the paraquat in the beverages includes the steps of diluting samples of beverages such as fruit juice and vegetable juice, using gold nanoparticle sol as a substrate, and detecting the paraquat in the samples through surface-enhanced Roman spectroscopy. The method capable of quickly detecting the paraquat in the beverages has the advantages that the residual paraquat in the samples can be detected qualitatively and quantitatively quickly and effectively; the method is high in sensitivity, low in detection limit and capable of detecting the 0.1 microgram / mL paraquat effectively only within a few minutes, and can only detect the above 5 micrograms / mL paraquat in undiluted samples, so that the paraquat can be detected quickly, practically, efficiently and economically.
Owner:SHANGHAI OCEAN UNIV
Food and oil grain acoustic signal feature extraction method and system based on wavelet transformation
InactiveCN102455327ALow cost detectionObjective detectionAnalysing solids using sonic/ultrasonic/infrasonic wavesProcessing detected response signalImage resolutionNondestructive testing
The invention discloses a food and oil grain acoustic signal feature extraction method and a food and oil grain acoustic signal feature extraction system based on wavelet transformation. The method comprises steps that: food or oil grains are delivered grain-by-grain into an acoustic signal collection box; the grains impact a metal target in the collection box, such that acoustic signals are produced; the impaction acoustic signals are received by a sound transducer; the received acoustic signals are processed through filtering and amplifying, and are transmitted to a data collection card; the acoustic signals are subject to A / D conversion in the data collection card, and are stored in the data collection card; the acoustic signals are decomposed with the wavelet transformation technology; the acoustic signals are reconstructed by using a selected specific wavelet coefficient; energy values of reconstructed signals are extracted as features, and are associated with quality features ofthe food or oil grains. According to the invention, with the acoustic features of the food and oil grains, and combined with the wavelet transformation technology, a non-destructive test is carried out upon the quality features of food and oil grains. The method is convenient and fast. The testing result is stable, and the resolution is relatively high.
Owner:HENAN UNIVERSITY OF TECHNOLOGY
Online surface defect detection method based on photometric stereo method
InactiveCN112630230ARealize online detectionSimple structureOptically investigating flaws/contaminationTemplate matchingFeature extraction
The invention relates to an online surface defect detection method based on a photometric stereo method, which comprises the following steps: in the detection process, parts continuously move along with a conveyor belt, when the parts reach a detection area, light sources distributed around the detection area are circularly lightened, and meanwhile, a camera synchronously shoots pictures under the illumination conditions of the light sources in different directions; wherein in the whole process, the parts continuously move along with a conveying belt, in the process of shooting different pictures, and the positions of the parts move; the surface defect detection step comprises the steps that a template is manufactured by extracting edge information, and unification of the positions of the parts in the multiple pictures is achieved according to template matching so as to meet the precondition of subsequent photometric stereo method calculation; then the gradient and curvature information of the surface of the part are recovered under different stations through a photometric three-dimensional method; the surface defects of the part are extracted through binarization, edge detection and connected domain detection feature extraction means in sequence, and preliminary screening is conducted according to a threshold value to judge whether the machining quality of the part to be detected is qualified or not.
Owner:TIANJIN UNIV
Surface enhance Raman spectroscopy (SERS) based acetylcholine detection method
ActiveCN107884386ALow cost detectionEasy to operateRaman scatteringOrganic moleculesMolecular physics
The invention discloses a surface enhance Raman scattering based acetylcholine detection method. According to the method, the competition between acetylcholine and organic molecules on a metal nano substrate is utilized, under the assistance of SERS, corresponding relationship between surface enhanced Raman strength of organic molecules and acetylcholine concentration is established, and highly sensitive, low cost, simple, and rapid acetylcholine detection is realized.
Owner:SOUTHEAST UNIV
Composite nano-probe based on N-Ti<3>C<2>QDs and o-phenylenediamine oxide, and ratio fluorescence detection method thereof
ActiveCN110726707ASingle excitation double emissionHigh fluorescence quantum yieldMaterial nanotechnologySilicon carbidePurine-Xanthine OxidasePhotoinduced electron transfer
The invention discloses a composite nano-probe based on N-Ti<3>C<2>QDs and o-phenylenediamine oxide, and discloses a ratio fluorescence detection method of the probe for H<2>O<2> and xanthine. The probe is the composite nano-probe, which is formed by bonding N-Ti<3>C<2>QDs to DAP through pi-pi conjugation and hydrogen bonds and used for ratio fluorescence detection of H<2>O<2> or xanthine. According to the invention, a dual-emission reverse ratio fluorescent sensing platform of H<2>O<2> based on photo-induced electron transfer (PET) is established by using quantum dots synthesized by a MXene two-dimensional material for the first time; furthermore, on the basis of the effect of xanthine generating H<2>O<2> under specific catalysis of xanthine oxidase, ratio fluorescence detection of xanthine is realized; and the detection method in the invention has the advantages of being free from labelling, high in sensitivity, high in accuracy and low in cost detection, and has good application prospect.
Owner:NANJING MEDICAL UNIV
Simple electrochemiluminescence detection method of glyphosate pesticide
InactiveCN107247079AQuantitative determination of contentDetermination of contentChemiluminescene/bioluminescenceMaterial electrochemical variablesCarbon nitrideElectrochemiluminescence
The invention belongs to the field of pesticide detection, and discloses a simple electrochemiluminescence detection method of glyphosate pesticide, and especially relates to preparation and applications of a carbon nitride-based glyphosate electrochemiluminescence sensor. According to a preparation method, graphene carbon nitride (g-C3N4) is used for modifying glassy carbon electrode loaded with Ag<+> on the surface; Ag<+> is released from the g-C3N4 modified electrode surface under the complexing action of glyphosate in a solution with Ag<+> on the surface of the electrode, recovery of electroluminescent signals is realized, and detection of glyphosate is realized via detecting the electroluminescent signals.
Owner:UNIV OF JINAN
Method for detecting brain natriuretic peptide through composite probe fluorescence "turning on-turning off-turning on" strategy on basis of cobalt nanomaterial/nucleic acid aptamer
InactiveCN110095616AReduce background signalLow cost detectionBiological material analysisBiological testingFluorescenceDiagnostic Specificity
The invention discloses a method for detecting brain natriuretic peptide through a composite probe fluorescence "turning on-turning off-turning on" strategy on the basis of cobalt nanomaterial / nucleicacid aptamer. According to the method, a CoOOH nano-ring with a high-efficiency fluorescence quenching effect is synthesized; a CoOOH nano-ring / FAM-aptamer composite probe is constructed; backgroundsignals are reduced by means of the fluorescence quenching effect of the CoOOH nano-ring for a dye; the fluorescent dye can be made to move away from the CoOOH nano-ring by means of the recognition effect of the BNP (brain natriuretic peptide) for the specific molecules of FAM-aptamer, so that fluorescent signals can be recovered; and fluorescence recovery efficiency and BNP concentration are in agood linear relationship in a range of 0.030 to 0.80pg / mL. The method has the advantages of simplicity, rapidness, high sensitiveness, high specificity and the like, and can be used for the quantitative detection of BNP in clinical specimens. ROC curve analysis shows that the method has high accuracy in diagnosing heart failure (AUC=0.87, 95% CI: 0.71-1.00, and P<0.01), and the method has good specificity (diagnostic specificity is 87. 9%); and the use cost of the method is greatly reduced. The method is suitable for being widely promoted and used in the diagnosis and prognosis of the heart failure of children and adults in primary-level organizations.
Owner:CHONGQING MEDICAL UNIVERSITY
Detection device of excircular surface defect of tapered roller
InactiveCN109001229AHigh-speed and reliable automatic detectionSimple structureMaterial analysis by optical meansEngineeringLight source
The invention relates to a detection device of an excircular surface defect of a tapered roller. The detection device comprises a camera, a light source, a conveying mechanism and a driving mechanismfor driving the conveying mechanism to move, wherein the camera is used for shooting an image of a rolling body in the rolling process under coordination of the light source; the detection device is characterized in that at least one hole slot channel matched with the size and the position of a rolling body to be detected is formed in the conveying mechanism along the advancing direction of the rolling body to be detected; the conveying mechanism is placed on a stationary bottom plate; the rolling body to be detected is the tapered roller; the conveying mechanism is a trapezoidal hole slot array structure turnplate; the driving mechanism is an electric turntable; the hole slot channel consists of a plurality of trapezoidal hole slots which are formed around the circular center of the turnplate as a center; the trapezoidal hole slots are designed according to the size and the geometric structural features of the tapered roller; when the electric turntable drives the trapezoidal hole slot array structure turnplate to rotate, due to a friction between the tapered roller and the bottom plate, the tapered roller overturns in the hole slot.
Owner:TIANJIN UNIV
Method and system for acquiring elasticity modulus of rock containing sedimentary rhythm layer
ActiveCN113624603ALower requirementNo damageMaterial analysis using wave/particle radiationEarth material testingLithologyRhythmite
The invention discloses a method and a system for acquiring the elasticity modulus of rock containing a sedimentary rhythm layer. The method comprises the following steps: acquiring a rock sample containing the sedimentary rhythm layer; measuring the rock element content of the rock sample at the test points by using a fluorescence spectrometer; wherein the test points are arranged on different rhythm layers of the rock sample; determining the lithology of the rock sample according to the rock element content; determining an element-mineral relation equation according to the lithology; determining mineral components of the rock sample by utilizing the lithology and the element-mineral relation equation; determining a mineral modulus coefficient according to the mineral components; and determining the elastic modulus of the rock sample according to the mineral components and the mineral modulus coefficient. According to the invention, nondestructive testing of mechanical properties of the rock sample can be realized.
Owner:INST OF GEOLOGY & GEOPHYSICS CHINESE ACAD OF SCI
Physiological sample detection method and equipment
ActiveCN112230005ASave internal spaceReduce volumeChemiluminescene/bioluminescenceBiological testingReliability engineeringElectrical and Electronics engineering
The invention belongs to the technical field of physiological sample detection, and provides a physiological sample detection method which comprises the following steps: disposable chip boxes are respectively or simultaneously moved to corresponding set stations on the same detection platform along at least two linear paths, or along at least one linear path and at least one arc path, or along atleast two arc paths; and the physiological samples in the disposable chip box are correspondingly detected at the corresponding set stations, so that the integration and expansion of various detectionmethods are realized, the technical problems of high detection cost and huge detection equipment volume caused by the validity period of bottle opening are effectively solved, and high-efficiency andlow-cost detection is realized.
Owner:SHENZHEN LIVING WATER POCT CO LTD
Multi-layer synchronous measurement method for curved surface light-transmitting piece
ActiveCN110702002AReliable detectionShort error propagation chainImage analysisUsing optical meansEngineeringImage pair
The invention relates to a multi-layer synchronous measurement method for a curved surface light-transmitting piece. The method comprises the following steps: fixing a to-be-measured curved surface light-transmitting piece on a horizontal displacement table, and collecting images; performing closed operation on the images, and filling small holes in an upper contour; performing open operation on the images, and removing burrs on the images; enabling each image to present an upper contour and a lower contour of the curved surface light-transmitting piece, and extracting corresponding pixel positions of the upper contour and the lower contour in the image; substituting the extracted pixel coordinates into a calibration relational expression; and processing the plurality of images to obtain three-dimensional information of the whole to-be-measured piece.
Owner:TIANJIN UNIV
Method for rapidly detecting RNA viruses based on CCP-FRET
InactiveCN112322793ALow cost detectionMicrobiological testing/measurementAgainst vector-borne diseasesVirusPolymer
The invention discloses a method for rapidly detecting RNA viruses based on CCP-FRET. The invention provides a method for detecting whether a to-be-detected sample contains target nucleic acid. The method sequentially comprises the following steps: (1) taking the to-be-detected sample or nucleic acid extracted from the to-be-detected sample as a template, taking a target nucleic acid as a target fragment for PCR amplification, and carrying out PCR amplification in a system containing dye-labeled dNTP to obtain a dye-labeled PCR amplification product; and (2) mixing the dye-labeled PCR amplification product with a conjugated polymer, and judging whether the to-be-detected sample contains target nucleic acid or not by detecting fluorescence energy resonance transfer. According to the invention, a conjugated polymer-based fluorescence energy resonance transfer technology is adopted to establish the method for detecting whether the to-be-detected sample contains the target nucleic acid ornot, such that rapid, visual and low-cost detection can be achieved.
Owner:INST OF CHEM CHINESE ACAD OF SCI +1
Novel coronavirus detection kit and detection method thereof
PendingCN112048574AAvoid Card Issue StructuresAvoid non-specific bindingMicrobiological testing/measurementAgainst vector-borne diseasesViral testNasopharyngeal aspirate
The invention provides a novel coronavirus detection kit and a detection method thereof, and belongs to the technical field of molecular biological detection. A series of primer probe groups for detecting the novel coronavirus are redesigned, an existing detection method is improved, and detection targets are further increased, so that the detection sensitivity of the novel coronavirus is improved, false negative results can be remarkably reduced especially for detection of low-copy-number samples, the sensitivity and the accuracy of detection are effectively improved, and high-efficiency, high-specificity and low-cost detection is realized. The kit can be used for qualitatively detecting the novel coronavirus genes in pneumonia suspected cases and suspected aggregated case patients infected by the novel coronavirus in vitro and samples such as nasopharyngeal swabs and sputum of other patients needing novel coronavirus infection diagnosis or differential diagnosis.
Owner:北京吉检医疗科技有限公司
Detection method for starting material A in bulk drug of landiolol hydrochloride
ActiveCN106248825AMeet the testing requirementsEasy to operateComponent separationInternal standardLandiolol
The invention discloses a detection method for a starting material A in the bulk drug of landiolol hydrochloride. The detection method comprises the following steps: pretreatment of the bulk drug of landiolol hydrochloride; liquid chromatographic-mass spectrometric detection of the pretreated bulk drug of landiolol hydrochloride; and quantitative analysis of the starting material A in the bulk drug of landiolol hydrochloride by using an internal standard method. The method has detection sensitivity of 0.50 mg / kg, and the precision and recovery rate of the method meets detection requirements on (S)-(+)-glycidyl nosylate. The method is simple to operate and reliable in results and can realize rapid and low-cost detection of trace (S)-(+)-glycidyl nosylate in the bulk drug of landiolol hydrochloride.
Owner:SHANDONG ANALYSIS & TEST CENT
Structural mode identification method based on computer vision and variational mode decomposition
ActiveCN113128474AHigh precisionSolve the limited measurement pointsSustainable transportationCharacter and pattern recognitionFrequency domain decompositionEngineering
The invention discloses a structural mode identification method based on computer vision and variational mode decomposition, and the method comprises the steps of collecting a vibration video of a structural object, and selecting pixel points meeting a preset pixel level in the vibration video as feature points; calculating the speed of each selected feature point by using a Farneback dense optical flow algorithm; calculating the speed and acceleration of each feature point in a real ground coordinate system by using a scale transformation mode to obtain an acceleration signal of a non-stationary sequence; performing noise reduction processing on the acceleration signal by using a variational mode decomposition method; and recognizing the acceleration signal after noise reduction processing by using a frequency domain decomposition method to obtain the vibration characteristics of the structure in each order mode. According to the invention, the vibration video of the structure is calculated and multi-modal extracted by using the improved optical flow algorithm in a non-contact manner, so that the real-time, efficient and low-cost detection of the dynamic characteristics of the structure is realized.
Owner:CHONGQING UNIV
Positioning precision detecting method and device for medical radiotherapy equipment
InactiveCN100403986CLow cost detectionCalibration apparatusStructural/machines measurementSensor arrayHand held
The present invention relates to method and device for detecting the positioning precision of medical radiotherapy equipment. The device includes one sampling unit for acquiring the position information of the irradiation points of the medical radiotherapy equipment, and one hand held controller for processing the acquired sampling information and sending sampling request command to the sampling unit. The sampling unit includes one casing, three turntables on the casing with one-dimensional sensor array to receiving the laser positioning information from three positioning laser lamps and the analog lighting information of the medical radiotherapy equipment. The present invention has low detection cost.
Owner:杨诚 +1
Paper-based detection method for exosome
ActiveCN112608983AEasy to operateLower requirementMicrobiological testing/measurementNucleic acid amplification techniqueSingle strand
The invention provides a paper-based detection method for exosome. The paper-based detection method employs (1) a recognition and molecular transformation system, (2) an amplification reaction system and (3) an LFB detection and analysis system. According to the invention, the exosome is converted into a single-stranded DNA target; the single-stranded DNA is amplified by a nucleic acid amplification technology; and the amplified single-stranded DNA is further detected by using lateral flow chromatography test paper which uses PtAu-NiO2 nano-enzyme as a signal probe. The nucleic acid lateral flow chromatography test paper is used as a terminal detection platform, so the problem that the exosome cannot be qualitatively and quantitatively detected at the same time is solved, and simple, convenient and low-cost detection of the exosome is realized.
Owner:CHINA AGRI UNIV
Thyroid dysfunction model and establishment method thereof
InactiveCN109346156AQuick cost testLow cost detectionMedical simulationMedical imagesDiseaseSerum ige
The invention relates to a thyroid dysfunction model and an establishment method thereof. The method comprises steps that (1), fresh blood from patients with the thyroid dysfunction and persons with the normal thyroid function are collected; serum is extracted to obtain serum samples; (2), the serum samples are detected through a Horiba confocal Raman spectrograph to obtain the serum Raman spectra; (3), fitting of all the serum autofluorescence background is performed, a baseline is corrected, and the corrected spectral data is subjected to integral area normalization processing to obtain thespectral data after processing; and (4), after the processed spectral data is subjected to dimensionality reduction and feature extraction through the PCA method, a SVM classification model is established, and the thyroid dysfunction model is obtained. The method is advantaged in the thyroid dysfunction model is established, based on the model, fast low-cost detection of thyroid diseases can be achieved.
Owner:THE FIRST TEACHING HOSPITAL OF XINJIANG MEDICAL UNIVERCITY
The detection method of starting material a in the raw material drug of landiolol hydrochloride
Owner:SHANDONG ANALYSIS & TEST CENT
A paper-based detection method for exosomes
ActiveCN112608983BEasy to operateLower requirementMicrobiological testing/measurementNucleic acid amplification techniqueSingle strand
The present invention provides a paper-based detection method for exosomes, including: (1) recognition and molecular conversion system, (2) amplification reaction system, and (3) LFB detection and analysis system. The present invention converts exosomes into single-stranded DNA targets, amplifies the single-stranded DNA through nucleic acid amplification technology, and further utilizes PtAu-NiO 2 The amplified single-stranded DNA is detected by the lateral flow chromatography test paper with the nanozyme as the signal probe. The invention uses nucleic acid lateral flow chromatography test paper as the terminal detection platform, solves the problem that exosomes cannot be detected qualitatively and quantitatively at the same time, and realizes simple and low-cost detection of exosomes.
Owner:北京红果源生物技术有限公司
Preparation and visual detection method of a core-shell magnetic multicolor fluorescent probe
ActiveCN107488447BAvoid quenchingReduce dosageFluorescence/phosphorescenceLuminescent compositionsTectorial membraneHalloysite
Owner:HENAN POLYTECHNIC UNIV
K-ras gene mutation site detection kit
ActiveCN110835644ARealize detectionImprove resolutionMicrobiological testing/measurementDNA/RNA fragmentationGene MutantGene
The invention relates to the field of molecular biotechnology and gene detection, in particular to a K-ras gene mutation site detection kit, comprising: a), a primer pair; b), an upstream probe, downstream probes, and a hairpin probe; and c), two nanogold probes. The above-mentioned primers and probes are suitable for carrying out high-sensitivity high-resolution low-cost detection on K-ras gene mutation sites under closed tube condition, so that cross contaminations to amplification products can be effectively avoided can be effectively avoided, wherein the downstream probes are suitable forindividual use and also for use with each other to carry out multiple detection in a same reaction system, so that the kit herein is suitable for detecting up to seven K-ras gene mutation sites at thesame time, and the detection efficiency is higher.
Owner:广州市宝创生物技术有限公司
A method for the detection of acetylcholine based on surface-enhanced Raman spectroscopy
ActiveCN107884386BAchieve high sensitivityLow cost detectionRaman scatteringSurface-enhanced Raman spectroscopyPhotochemistry
The invention discloses a surface enhance Raman scattering based acetylcholine detection method. According to the method, the competition between acetylcholine and organic molecules on a metal nano substrate is utilized, under the assistance of SERS, corresponding relationship between surface enhanced Raman strength of organic molecules and acetylcholine concentration is established, and highly sensitive, low cost, simple, and rapid acetylcholine detection is realized.
Owner:SOUTHEAST UNIV
A method for the simultaneous detection of bod and nitrate nitrogen based on electrochemically active microorganisms
ActiveCN111175356BNo cumbersome replacementLow cost detectionMaterial electrochemical variablesMicroorganismWater quality
The invention relates to a method for synchronously detecting biochemical oxygen demand and nitrate nitrogen content in a water body based on bidirectional electron transfer electrochemical active microorganisms and mainly aims to solve problems of detection hysteresis, complex operation, high cost and incapability of synchronous detection in the water body water quality biochemical oxygen demandand nitrate nitrogen detection process in the prior art. The method is advantaged in that a bioelectrochemical system taking electrochemical active microorganisms with bidirectional electron transfercapability as a core is constructed, step potentials are set to measure the electricity generation capacity and the electrophilic capacity of the system respectively, and rapid real-time detection ofbiochemical oxygen demand and nitrate nitrogen in the water body can be realized by utilizing an output current signal during electricity generation of the system, an input current signal during electrophilic and a standard curve model of biochemical oxygen demand and nitrate nitrogen.
Owner:BEIHANG UNIV
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