34 results about "Diagnostic Specificity" patented technology

The invention belongs to the technical field of biomedical engineering, and relates to an integration imaging system used for early stage cervical carcinoma detection and integrating fluorescent mesoscope imaging and optical coherence tomography (OCT). The integration imaging system comprises a fluorescent mesoscope imaging system and a spectrum OCT system. The fluorescent mesoscope imaging system comprises a laser light source, a polarizer, a polarized light spectroscope, a first dichroscope, a second dichroscope, an X-Y scanning galvanometer, an objective lens, a fluorescent detection part, a diffused light detection part and a computer, wherein the X-Y scanning galvanometer and the objective lens are shared by the spectrum OCT system. The spectrum OCT system comprises a low-coherent light source, a fiber polarization splitter, a focusing lens, the X-Y scanning galvanometer, the objective lens and a spectrograph. Ultraviolet light beams generated by the laser light source pass through the polarizer, the polarized light spectroscope and the first dichroscope and then are reflected through the second dichroscope, and samples generated by the low-coherent light source are collimated through the focusing lens and are combined with the ultraviolet light beams. The system improves diagnostic specificity through mutual evidence of organization function information and histomorphology information.

Featured are methods and systems to multiparametric non-linear dimension reduction (NLDR) methods for segmentation and classification of radiological images. Such methods for segmentation and classification of radiological images, includes pre-processing of acquired image data; and reconstructing the acquired image data using a non-linear dimension reduction technique so as to yield an embedded image representing all of the acquired, where the acquired image data comprises a plurality of different sets of image data of the same region of interest. Such NLDR methods and systems are particularly suitable for the ability to combine multiple input images into a single unit for increased specificity of diagnosis.

The invention discloses a method for detecting brain natriuretic peptide through a composite probe fluorescence "turning on-turning off-turning on" strategy on the basis of cobalt nanomaterial / nucleicacid aptamer. According to the method, a CoOOH nano-ring with a high-efficiency fluorescence quenching effect is synthesized; a CoOOH nano-ring / FAM-aptamer composite probe is constructed; backgroundsignals are reduced by means of the fluorescence quenching effect of the CoOOH nano-ring for a dye; the fluorescent dye can be made to move away from the CoOOH nano-ring by means of the recognition effect of the BNP (brain natriuretic peptide) for the specific molecules of FAM-aptamer, so that fluorescent signals can be recovered; and fluorescence recovery efficiency and BNP concentration are in agood linear relationship in a range of 0.030 to 0.80pg / mL. The method has the advantages of simplicity, rapidness, high sensitiveness, high specificity and the like, and can be used for the quantitative detection of BNP in clinical specimens. ROC curve analysis shows that the method has high accuracy in diagnosing heart failure (AUC=0.87, 95% CI: 0.71-1.00, and P<0.01), and the method has good specificity (diagnostic specificity is 87. 9%); and the use cost of the method is greatly reduced. The method is suitable for being widely promoted and used in the diagnosis and prognosis of the heart failure of children and adults in primary-level organizations.

A universally usable method for specific detection of target nucleic acid sequences, which method can be performed very rapidly and also simply and furthermore which does not need any expensive instrumental systems. The method is intended to be suitable as a molecular genetic rapid test and to respect the requirements of diagnostic specificity assurance. In this regard it is important that only one specific amplification product be detected and that amplification artifacts can be unambiguously discriminated. A nucleic acid amplification kit suitable for performing this method.

The invention relates to the technical field of auxiliary diagnosis of diseases, and discloses an enteric microorganism for diagnosing cardiac valve calcification, which comprises one or more of Verrucomicrobiaceae or Prevotella or Bacteroides or Veillonella. The invention further discloses an application of the enteric microorganism for diagnosing cardiac valve calcification in preparation of a kit for diagnosing the cardiac valve calcification. The invention also discloses an application of the enteric microorganism for diagnosing cardiac valve calcification in preparing a chip for diagnosing cardiac valve calcification. The invention also discloses a primer group for detecting the enteric microorganisms. According to the invention, the level change of Verrucomicrobiaceae or Prevotella or Bacteroides or Veillonella in intestinal flora in excrement can be used for diagnosing the cardiac valve calcification; rapid diagnosis of the cardiac valve calcification is achieved, diagnosis is easy, convenient and rapid, invasion is avoided, cost is low, diagnosis specificity is high, sensitivity is high, an application object is suitable for large-scale crowd screening, and final monitoringcan be achieved for individuals.

The invention belongs to the technical field of biology, and particularly relates to a molecular diagnosis technology for adenomyosis. According to the technical scheme, the miRNA kit for diagnosing adenomyosis comprises any combination of reverse transcription primers of hsa-miR-22-3p, hsa-miR-182-5p and hsa-miR-103a-3p, a reagent for a probe method qPCR reaction, namely a probe and forward and reverse PCR primers. The kit can also comprise a single-chain RNA standard substance of the hsa-miR-22-3p, the hsa-miR-182-5p and the hsa-miR-103a-3p. A serum miRNAs expression database and a specific marker, namely any combination of hsa-miR-22-3p, hsa-miR-182-5p and hsa-miR-103a-3p, related to adenomyosis incidence are obtained through a large amount of deep research in the early stage. Then any two of the hsa-miR-22-3p, the hsa-miR-182-5p and the hsa-miR-103a-3p are detected through development and application of the specific diagnostic kit. As a diagnostic specificity index, the diagnosis of adenomyosis can be more convenient and easy to implement.

The present invention relates to an application of amine metabolites in serum: aspartic acid, ornithine, hypoxanthine, phenylalanyl phenylalanine and glutamine phenylalanine as combination markers in preparation of a kit for early hepatic encephalopathy paints for diagnosis subjects, The invention also relates to the kit for detecting early hepatic encephalopathy paints for diagnosis subjects, by detecting relative concentration of the amine metabolism marker in serum of the diagnosis subjects, based on a binary logistic regression equation to calculate combination marker variate Prob and estimate intercept point value, so that whether the diagnosis subject is early hepatic encephalopathy paint or not can be determined. The kit has the characteristics of low detection cost and good stability. The kit can be used for clinical diagnosis for early hepatic encephalopathy, and has characteristics of high diagnostic specificity and high sensitivity, has characteristics of complementation with traditional clinical diagnostic marker blood ammonia, and has high development and application values.

The invention relates to the technical field of molecular biological detection, in particular to an esophagus cancer diagnosis specific expression pattern based on serum exosomallncRNAs and a detecting and analyzing system. The expression pattern consists of UCA1 and POU3F3 which are used for expressing up-regulation. The early esophageal cancer IncRNAs specific expression pattern detecting and analyzing system comprises a quantitative detection module, an input module, an analysis module and an output module, wherein the analysis module comprises logit module calculation and ROC (Receiver Operating Characteristic) curve analysis; the logit model is shown as logit(P=ESCC)=-1.1338+0.3957*U+0.2561*P, wherein the U is a UCA1 expression number value, P is POU3F3 expression number value. The detecting and analyzing system has high diagnosis efficiency specific to the esophagus cancer which is higher than that of the conventional serum SCCA, the model can be used for diagnosing patients suffering from early esophageal cancer (I and II).

The invention provides a DcR3 polypeptide for treating infectious diseases and applications thereof. Concretely, DcR3 is a marker for indicating infectious inflammation with high specificity and sensitivity, and inhibition of DcR3 has effective treatment effects for infectious inflammation. The polypeptide of DcR3 is designed and synthesized for competitive inhibition of combination of apoptosis acceptors (such as Fas, HVEM / LT R, DR3, etc.) and apoptosis factors (FasL, LIGHT, TL1A, etc.), so that apoptosis pathway is blocked, and biological effects of infectious inflammation (such as septicemia) symptoms can be effectively reduced. In addition, experiments also show that when DcR3 and PCT are used as markers at the same time, specificity and sensitivity of early diagnosis of septicemia are improved.

We have developed a modified indirect ELISA (MI-ELISA) using the spherical body protein-4 (SBP4) of Babesia bovis to detect antibody against diverse isolates through all infection stages in cattle. This SBP4 MI-ELISA was evaluated for sensitivity and specificity against field sera and sera from cattle infected experimentally with various doses and isolates as well as in detecting acute and persistent infection. The diagnostic specificity of the SBP4 MI-ELISA using IFA-negative sera was 100%, significantly higher than the RAP-1 cELISA (90.4%); the diagnostic sensitivity of the SBP4 MI-ELISA was 98.7% using the IFA-positive sera, in contrast to that of the RAP-1 cELISA at 60%. Results demonstrate excellent diagnostic sensitivity and specificity of the novel SBP4 MI-ELISA for cattle with acute and long-term carrier infections. Use of the SBP4 MI-ELISA assay in countries that have B. bovis-endemic herds will be pivotal in preventing the spread of this disease to non-endemic herds.

The application relates to the field of biological detection, and specifically discloses a sealing agent for biological detection, a preparation method thereof, a coated plate and a kit using the coated plate. Each liter of sealing agent is made of the following raw materials: 2.5-9g of water-soluble alcoholic organic matter, 0-1.1g of polyglucosamine, 0-0.7g of gelatin, 0.1-0.4g of antioxidant, 0.07-0.2g of preservative, and the balance It is water; the water-soluble alcoholic organic matter is selected from at least two of ethylene glycol, polyvinyl alcohol and polyethylene glycol. The preparation method of the sealing agent comprises the following steps: uniformly mixing water-soluble alcoholic organic matter, polyglucosamine, gelatin, antioxidant, preservative and water according to the formula quantity. The coated plate is prepared after being sealed with the above sealing agent, and the kit includes the above prepared coated plate. The use of the blocking agent of the present application can effectively reduce the non-specific reaction in the detection process, with few false positive results and high diagnostic specificity.

The invention belongs to the technical field of biomedical engineering, and relates to an integration imaging system used for early stage cervical carcinoma detection and integrating fluorescent mesoscope imaging and optical coherence tomography (OCT). The integration imaging system comprises a fluorescent mesoscope imaging system and a spectrum OCT system. The fluorescent mesoscope imaging system comprises a laser light source, a polarizer, a polarized light spectroscope, a first dichroscope, a second dichroscope, an X-Y scanning galvanometer, an objective lens, a fluorescent detection part, a diffused light detection part and a computer, wherein the X-Y scanning galvanometer and the objective lens are shared by the spectrum OCT system. The spectrum OCT system comprises a low-coherent light source, a fiber polarization splitter, a focusing lens, the X-Y scanning galvanometer, the objective lens and a spectrograph. Ultraviolet light beams generated by the laser light source pass through the polarizer, the polarized light spectroscope and the first dichroscope and then are reflected through the second dichroscope, and samples generated by the low-coherent light source are collimated through the focusing lens and are combined with the ultraviolet light beams. The system improves diagnostic specificity through mutual evidence of organization function information and histomorphology information.

The present inventions provides combinations of primers and probes for performing quantitative PCRs that can be used to determine the methylation state and level of BMP3 gene and NDRG4 genes in a patient in need thereof, which leads to surprisingly high diagnostic specificity and sensitivity for diagnosing the presence or the absence of colorectal cancer (CRC) and / or advanced adenoma (AA) in a patient in need therefore. Compositions and methods for performing the diagnosis are provided.