58 results about "Babesia" patented technology

The invention relates to a membrane for detecting cattle and sheep Giardia in Haemaphysalis qinghaiensis and distinguishing the type. The membrane for detecting Theileria luwenshuni, Theileria uilenbergi and Giardia in cattle and sheep is prepared by fixing specific oligonucleotide of the Theileria luwenshuni and the Theileria uilenbergi or the specific oligonucleotide of Babesia and the universal specific oligonucleotide of Theileria on a Biodyne C membrane. The method for detecting comprises the steps: using DNA extracted from Haemaphysalis to be detected as a template; using a specific primer to perform PCR amplification; hybridizing the product with the oligonucleotide of the Theileria and Babesia for detecting; putting the hybridized membrane in an exposed template holder after enhanced chemiluminescence incubation; putting an X-ray film on the membrane for treatment of exposure, developing and the like; and determining whether the detected animal has the corresponding disease according to an intensity signal produced on the film.

The invention discloses a primer, a probe and a kit for fluorescence quantitative PCR (Polymerase Chain Reaction) detecting and typing of babesia. According to the invention, the primer sequence is as shown in SEQID (SEQuence IDentifier) NO.1, 2 and 3 and the probe is as shown in SEQIDNO.4 and 5. A typing method of the babesia comprises the following steps of: carrying out PCR amplification on the to-be-detected bacterium by using the primer and the probe to obtain Erich bacterium positive samples of 290bp (base pair) bands, wherein the fluorescence of the samples is enhanced at the wavelength of 640nm in fluorescence detection; and typing the babesia according to the melting temperature. The primer, the probe and the kit disclosed by the invention have obvious technical advantages, have very high specificity and sensitivity and can be used for conveniently detecting the babesia of important species.

The present invention relates to methods for stable transfection of Babesia parasites, and for vaccines conferring immunity against parasitic arthropods.

The invention relates to the technical field of molecular biomedicine, in particular to a PCR (Polymerase Chain Reaction) detection kit for cat and / or dog pathogens, a detection method and application. The kit is used for detecting canine distemper virus, canine influenza A virus, canine parainfluenza virus, canine parvovirus, canine coronavirus, canine rotavirus, canine babesia, canine ascaris, canine Ehrlichia, canine brucella, rabies virus, borrelia burgdorferi, reference gene ACTB, feline herpes virus, feline calicivirus and feline parvovirus. The kit comprises primers and probes of feline coronavirus, feline immunodeficiency virus, feline leukemia virus, feline mycoplasma, feline mycoplasma, feline chlamydia, giardia, toxoplasma, bartonella and reference gene GAPDH, collected DNA and RNA are added into the kit, a real-time fluorescence PCR instrument is adopted for PCR reaction, FAM, HEX, ROX and CY5 fluorescence signals are collected in each cycle, analysis of related pathogens is carried out, and the kit can be used for detecting the feline and the canine. Compared with a traditional detection method, the method has the advantages of higher specificity and higher sensitivity.

Methods for the rapid detection of the presence or absence of Babesia in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targeting Babesia and kits are provided that are designed for the detection of Babesia, including, but not limited to, the Babesia species of B. microti, B. divergens, B. duncani, and B. venatorum. Also described are kits, reaction mixtures, and oligonucleotides (e.g., primer and probe) for the amplification and detection of Babesia.

Methods and compositions for preventing and / or treating diseases and conditions caused by certain apicomplexan infections are provided. The methods involve administering / providing to a subject one or more of tartrolon D and E, including isomers thereof. Exemplary apicomplexan infections that are prevented / treated by the compounds of the invention include those caused by Cryptosporidium, Babesia, Cyclospora, Isospora, Plasmodium, Sarcocystis, Besnoitia, Hammondia, Neospora, Theileria and Toxoplasma.

The invention discloses a babesia mocroti 2D41 antigen protein and application thereof. The protein has an amino acid sequence as shown by SEQ ID NO:8, and has an encoded gene sequence as shown by SEQID NO:7. The babesia mocroti 2D41 antigen protein has a continuous high-immunity reaction with mouse serum of 14dpi-270dpi in a specific IgG antibody reaction and has an effect of remarkably reducingparasitemia in immunity protection testing, and the average parasitemia value of the protein is reduced by about 35% when being compared with those of an adjuvant group and a natural immunity group.The 2D41 antigen protein can be used for detecting or diagnosing babesia mocroti diseases, and for preparing treatment and / or prevention medicines for reducing parasitemia.

The present invention provides an isolated gene, the sequence of which is shown in SEQ ID NO:3. The present invention also provides a Babesia microti Bm1524 antigenic protein, which is encoded by the above isolated gene, and its amino acid sequence is shown in SEQ ID NO:4. The present invention also provides an antibody that specifically binds to the above-mentioned Babesia microti Bm1524 antigen protein. The present invention has further established a highly specific and sensitive diagnostic method for Babesia microti through the expression and purification of Babesia microti Bm1524 antigen, which can be applied to different aspects such as scientific research and prevention of Babesia microti; the present invention The vaccine has the effect of preventing Babesia vole infection. The kit and the colloidal gold immunochromatographic test strip of the invention can also be used to detect Babesia microti.