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Esophageal cancer diagnosis-specific expression map and detection analysis system based on serum exosomal lncRNAs

A technology of specific expression profile and analysis system, applied in the field of esophageal cancer diagnosis specific expression profile and detection and analysis system, can solve the problem of low sensitivity and specificity in early diagnosis of esophageal cancer, limited wide application, low sensitivity and difficult to find esophagus, etc. question

Active Publication Date: 2020-07-28
THE SECOND HOSPITAL OF SHANDONG UNIV
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  • Description
  • Claims
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Problems solved by technology

Clinically, imaging barium meal examination is the most commonly used method for early screening of esophageal cancer. However, due to its low sensitivity, it is difficult to find small lesions in the esophagus, which has certain limitations for early diagnosis of esophageal cancer. High diagnostic efficiency, but the invasiveness of this examination method limits its wide application in esophageal cancer screening; currently, SCCA and CEA in serum are traditional markers commonly used in clinical esophageal cancer examination, but their significance for early esophageal cancer Both sensitivity and specificity of diagnosis are low

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  • Esophageal cancer diagnosis-specific expression map and detection analysis system based on serum exosomal lncRNAs
  • Esophageal cancer diagnosis-specific expression map and detection analysis system based on serum exosomal lncRNAs
  • Esophageal cancer diagnosis-specific expression map and detection analysis system based on serum exosomal lncRNAs

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Embodiment 1

[0047] 1. Research object

[0048] Serum samples were obtained from 159 patients with esophageal cancer and 159 controls who excluded esophageal cancer with the consent of the subjects. All patients with esophageal cancer were inpatients in the Department of Thoracic Surgery, Qilu Hospital of Shandong University. They were all diagnosed with esophageal cancer for the first time, and their pathological types were confirmed by postoperative pathological examination. All serum samples were obtained before patients received any medical or surgical treatment. The specimen collection was approved by the Medical Ethics Committee of Qilu Hospital of Shandong University, and the patients and their families gave informed consent.

[0049] 2. Specimen collection and processing

[0050] Collect 3mL of serum, immediately centrifuge at 1600g for 5min, then centrifuge at 16000g for 10min, use the separated supernatant for SCCA detection, and store the other part at -80°C for testing. In a...

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Abstract

The invention relates to the technical field of molecular biological detection, in particular to an esophagus cancer diagnosis specific expression pattern based on serum exosomallncRNAs and a detecting and analyzing system. The expression pattern consists of UCA1 and POU3F3 which are used for expressing up-regulation. The early esophageal cancer IncRNAs specific expression pattern detecting and analyzing system comprises a quantitative detection module, an input module, an analysis module and an output module, wherein the analysis module comprises logit module calculation and ROC (Receiver Operating Characteristic) curve analysis; the logit model is shown as logit(P=ESCC)=-1.1338+0.3957*U+0.2561*P, wherein the U is a UCA1 expression number value, P is POU3F3 expression number value. The detecting and analyzing system has high diagnosis efficiency specific to the esophagus cancer which is higher than that of the conventional serum SCCA, the model can be used for diagnosing patients suffering from early esophageal cancer (I and II).

Description

technical field [0001] The invention relates to the technical field of molecular biology detection, in particular to a diagnostic-specific expression map and detection and analysis system for esophageal cancer based on serum exosomallncRNAs. Background technique [0002] Esophageal cancer is one of the most common digestive tract tumors in the world, which seriously threatens human health. Therefore, early diagnosis and early treatment are the key to improve the prognosis and survival of patients with esophageal cancer and reduce the mortality rate. Currently, diagnostic methods for esophageal cancer mainly include barium meal, gastroscopy, tissue biopsy, and traditional serum tumor markers. Clinically, imaging barium meal examination is the most commonly used method for early screening of esophageal cancer. However, due to its low sensitivity, it is difficult to find small lesions in the esophagus, which has certain limitations for early diagnosis of esophageal cancer. Hi...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 王传新杜鲁涛闫素真蒋秀梅李娟
Owner THE SECOND HOSPITAL OF SHANDONG UNIV
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