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Detection method of specific gamma-glutamyl transferase and application thereof

A glutamyl and detection method technology, which is applied in the field of specific γ-glutamyl transferase specific detection, can solve the problems of lack of specificity detection method, low specificity of GGT total activity measurement, etc., and achieves broad application prospects. Effect

Inactive Publication Date: 2012-11-28
SHANGHAI DIAN CLINICAL TESTING CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the determination of serum GGT total activity is an enzymatic detection item carried out routinely in clinic, which is used in acute viral hepatitis, chronic hepatitis, post-hepatic cirrhosis, intrahepatic cholestasis, extrahepatic biliary obstruction, primary or In the case of metastatic liver cancer, they all increase to varying degrees; however, the specificity of the total activity of GGT in serum is low, and it cannot be used as a specific test for related diseases, especially for the differential diagnosis of liver cancer and other liver diseases. There is still a lack of specific detection methods and supporting products

Method used

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  • Detection method of specific gamma-glutamyl transferase and application thereof
  • Detection method of specific gamma-glutamyl transferase and application thereof

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Embodiment

[0020] 1. Prepare a specific γ-glutamyl transferase detection kit (10 copies), specifically as follows figure 1 As shown, it includes three tubes of reagents, namely:

[0021] Antibody liquid (1): anti-human Apo B antibody + buffer;

[0022] Buffer (2) pH7.0, 30mmol / L MOPS;

[0023] Precipitating agent (3): 30% polyethylene glycol 6000.

[0024] A. Reagent composition:

[0025] 1) Control group (total activity group): pH 7.0, 850 μL of 30 mmol / L MOPS buffer.

[0026] 2) Antibody group (group to be determined): 850 μL of anti-apolipoprotein B antibody and 420 μL of 30% polyethylene glycol 6000 precipitant.

[0027] B. Operation steps:

[0028] (1) Antibody group: Add 100 μL of patient’s serum sample, 80 μL of antibody solution, and 20 μL of precipitating agent into a centrifuge tube, place at 37°C for 20 minutes, centrifuge (5500g×5min), and take the supernatant to measure GGT activity on a biochemical analyzer.

[0029] (2) Control group: Add 100 μL of patient’s serum sa...

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Abstract

The invention provides a detection method of specific gamma-glutamyl transferase, which specifically detects the activity difference of the gamma-glutamyl transferase in serum before and after forming an immunoprecipitation bonder between an anti-apolipoprotein B antibody and low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL); as well as a diagnostic reagent or a diagnostic kit prepared by applying the method. Compared with the prior art, the invention provides a simple and effective diagnostic reagent or diagnostic kit for diagnosis or auxiliary diagnosis of liver cancer. Experiment results show that the specific method for detecting the activity of LDL / VLDL-GGT (gamma-glutamyl transferase) in the serum, provided by the invention, can be used for the diagnostic reagent or the diagnostic kit for detection or auxiliary detection of liver cancer, the average diagnostic specificity can be more than 70%, and the average positive predictive value is 90%. Therefore, the application prospects of the invention are very broad.

Description

technical field [0001] The invention relates to a specific detection method for specific gamma-glutamyl transferase and its application. Background technique [0002] The γ-glutamyltransferase (GGT, EC2.3.2.2) in serum exists in the form of isoenzymes or multiple molecules, and some GGT can be combined with lipoproteins, such as low-density lipoprotein (LDL ) and very low-density lipoprotein (VLDL), but its formation reasons and physiological functions are still unclear. In the prior art, the determination of serum GGT total activity is an enzymatic detection item carried out routinely in clinic, which is used in acute viral hepatitis, chronic hepatitis, post-hepatic cirrhosis, intrahepatic cholestasis, extrahepatic biliary obstruction, primary or In the case of metastatic liver cancer, they all increase to varying degrees; however, the specificity of the total activity of GGT in serum is low, and it cannot be used as a specific test for related diseases, especially for the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/48
Inventor 于嘉屏
Owner SHANGHAI DIAN CLINICAL TESTING CENT
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