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A method for the simultaneous detection of bod and nitrate nitrogen based on electrochemically active microorganisms

A technology of active microorganisms and simultaneous detection, applied in the direction of electrochemical variables of materials, etc., to achieve the effect of rapid and low-cost detection

Active Publication Date: 2021-03-16
BEIHANG UNIV
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  • Application Information

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Problems solved by technology

However, at present, there are still relatively few studies on the electrophilic process and mechanism of electrochemically active microorganisms, and the methods and technologies for detecting nitrate nitrogen in water using electrochemically active microorganisms have not yet been reported. and electrophilic function, it is expected to realize the simultaneous detection of biochemical organic matter and nitrate nitrogen in water, and its feasibility needs further study

Method used

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  • A method for the simultaneous detection of bod and nitrate nitrogen based on electrochemically active microorganisms
  • A method for the simultaneous detection of bod and nitrate nitrogen based on electrochemically active microorganisms
  • A method for the simultaneous detection of bod and nitrate nitrogen based on electrochemically active microorganisms

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Embodiment 1

[0029] First, a three-electrode bioelectrochemical system was constructed, including three electrodes (working electrode, counter electrode, and reference electrode) and an electrolytic cell. A 2.5cm×2.5cm carbon cloth (HCP330, Shanghai Hesen Electric Co., Ltd., China) was used as the working electrode. Before use, the carbon cloth was soaked in acetone overnight, dried and then ammoniated at high temperature. The working electrode is fixed by an electrode clip (J110, Tianjin Aida Hengsheng Technology Co., Ltd., China), with an effective area of ​​5.8 cm 2. Platinum electrode (Pt210, Tianjin Aida Hengsheng Technology Co., Ltd., China) and Ag / AgCl reference electrode (R0303, Tianjin Aida Hengsheng Technology Co., Ltd., China) and Ag / AgCl reference electrode (R0303, Tianjin Aida Hengsheng Technology Co., Ltd., China; 0.205 V vs. standard hydrogen electrode). The effective volume of the electrolytic cell is 50mL, and it is sealed by a PTFE cover. The cover is provided with 5 ho...

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Abstract

The invention relates to a method for synchronously detecting biochemical oxygen demand and nitrate nitrogen content in a water body based on bidirectional electron transfer electrochemical active microorganisms and mainly aims to solve problems of detection hysteresis, complex operation, high cost and incapability of synchronous detection in the water body water quality biochemical oxygen demandand nitrate nitrogen detection process in the prior art. The method is advantaged in that a bioelectrochemical system taking electrochemical active microorganisms with bidirectional electron transfercapability as a core is constructed, step potentials are set to measure the electricity generation capacity and the electrophilic capacity of the system respectively, and rapid real-time detection ofbiochemical oxygen demand and nitrate nitrogen in the water body can be realized by utilizing an output current signal during electricity generation of the system, an input current signal during electrophilic and a standard curve model of biochemical oxygen demand and nitrate nitrogen.

Description

technical field [0001] The invention relates to the field of water quality detection, in particular to water quality detection by electrochemically active microorganisms. Background technique [0002] With the rapid development of industrialization and urbanization in our country, people's living standards have been greatly improved, but as a by-product of industrialization and urbanization, the discharge of industrial wastewater and urban sewage has also risen sharply, and the pollution of water bodies has become increasingly widespread and serious. serious. According to the 2018 Environmental Bulletin, the quality of surface water in my country is still not optimistic. As an important source of surface water, 5 of the 7 major river basins have been polluted to varying degrees, and the Yellow River Basin, Songhua River Basin and Huaihe River Basin showed mild pollution, while the Haihe River Basin and the Liaohe River Basin have been moderately polluted and both have signif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30
CPCG01N27/26G01N27/30
Inventor 易越刘红谢倍珍藏雨轩赵婷
Owner BEIHANG UNIV
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