50results about How to "Avoid non-specific binding" patented technology

The invention discloses a preparation method for a polyclonal antibody to salmonella effect protein SopB. The invention aims to provide the preparation method for the rabbit-derived polyclonal antibody with good specificity, high sensitivity and capability of specifically binding to the endogenous salmonella effect protein SopB secreted in the process of salmonella infection of a host cell. A protein sequence as represented by SEQ ID No. 2 is used as immunogen, wherein the protein sequence consists of 139 amino acids located between position 29 and position 168, counted from terminal N, of a full-length amino acid sequence as represented by SEQ ID No. 1 of the salmonella effect protein SopB, and a conventional preparation method for a polyclonal antibody is used for preparation of antiserum; then the antiserum is purified so as to obtain the polyclonal antibody to the salmonella effect protein SopB. According to the invention, the polyclonal antibody to the salmonella effect protein SopB is prepared by using the method, and the polyclonal antibody is beneficial for immunoblotting analysis and immunofluorescent staining laser confocal microscopic observation of the salmonella effect protein SopB and for in-depth research on the infection mechanism of enteropathogens.

The invention relates to a graphene field effect transistor array biosensor and a preparation method and a detection method thereof. The invention relates to a biosensor and a preparation method and adetection method thereof, aiming at solving the problem of relatively low accuracy in the detection process of the existing graphene field effect transistor array biosensor. The sensor comprises a silicon substrate, an oxidization layer, a first metal grid electrode, a second metal grid electrode, a gate insulating layer, a first graphene conductive channel layer, a second graphene conductive channel layer, a first group source, a leakage electrode, a second group source and a leakage electrode; the preparation method comprises the following steps: I. making a transistor structure; II. performing aldehyde treatment on the graphene surface; III. modifying a detection unit graphene surface to capture antibody molecules; and IV. closing the remaining active sites on the graphene surfaces ofa detection unit and a reference unit; the detection method comprises the following steps: soaking the sensor in a solution containing be-detected antigen, and detecting the voltage at the graphene dirac point of the detection unit and the reference unit, to obtain the absolute concentration of the to-be-detected substance solution, wherein a difference value corresponds to a standard working curve.

The invention discloses a kit and application thereof in detection of a vascular endothelial growth factor. The kit contains the following components which are independently stored: an antibody-coated magnetic bead working solution, a VEGF labeled antibody, a VEGF calibrator, a VEGF quality control product and a sample diluent. The antibody-coated magnetic bead working solution is a magnetic bead suspension coated with an antibody A. The VEGF labeled antibody is obtained by labeling an antibody B through an acridinium ester modifier. The acridinium ester modifier is acridinium sulfonyl hydrazide amine inner salt or other acridinium ester derivatives, and the antibody A and the antibody B are humanized VEGF antibody Fab segments with different amino acid sequences. The acridinium ester modifier is selected to be coupled with the humanized anti-VEGF antibody Fab segments, so that the stability is high, the sensitivity and the detection range of a detection system are improved, the storage time of the detection system is prolonged, and the reaction time is shortened. The linear range of the kit is 0.5-50000 pg / mL.

The present invention relates to a display device using a semiconductor light emitting element and a method for manufacturing the same, and more particularly, to a display device using a semiconductor light emitting element having a size of several [mu] m to dozens of [mu] m and a method for manufacturing the same. The present invention provides a display device, which is characterized by comprising: a base part; a plurality of transistors disposed on the base part; a plurality of semiconductor light-emitting elements disposed on the base part; a plurality of wiring electrodes disposed on the base part and electrically connected to the plurality of transistors and the semiconductor light emitting element; a partition wall disposed on the base part and formed so as to cover the plurality of transistors; and a connection electrode connecting a portion of the plurality of transistors and a part of the plurality of wiring electrodes, the connection electrode being formed so as to penetrate the partition wall.

The invention discloses a production technology for preparing an NC (Nitrocellulose Filter) membrane by lineation. The production technology comprises the following working procedures: operating membrane lineation in a continuous state, and drying after membrane lineation; after the working procedure of drying after membrane lineation is carried out, sealing a membrane which operates under a continuous state, and drying after sealing, wherein the operation time of the working procedure of drying after membrane lineation is 2-4 min, and the operation time of membrane sealing and working procedure of drying after sealing is 3-6 min. The NC membrane obtained by the technical scheme of the invention can be used for effectively avoiding the diffusion and the widening of an antibody line, the detection fluorescence of the antibody line is centralized, meanwhile, the specificity combination of the antibody line is improved so as to obviously lower the intra-batch differences and the inter-batch differences of production, and the accuracy and the repeatability of a detection result are improved.