50results about How to "Avoid non-specific binding" patented technology

The present invention relates to a new method for the diagnosis of Helicobacter pylori infection. In particular, the present invention relates to novel non-invasive methods for detecting the presence in biological samples of H. pylori antigens or metabolites produced by said bacteria using biosensor-based measurements. The present invention also relates to the use of a biosensor comprising a Helicobacter pylori-specific antibody or an antigen-binding fragment thereof immobilized thereon and a biomolecule-repelling polymer that prevents non-specific binding. The invention also relates to detection kits used in the method.

The invention discloses a preparation method for a polyclonal antibody to salmonella effect protein SopB. The invention aims to provide the preparation method for the rabbit-derived polyclonal antibody with good specificity, high sensitivity and capability of specifically binding to the endogenous salmonella effect protein SopB secreted in the process of salmonella infection of a host cell. A protein sequence as represented by SEQ ID No. 2 is used as immunogen, wherein the protein sequence consists of 139 amino acids located between position 29 and position 168, counted from terminal N, of a full-length amino acid sequence as represented by SEQ ID No. 1 of the salmonella effect protein SopB, and a conventional preparation method for a polyclonal antibody is used for preparation of antiserum; then the antiserum is purified so as to obtain the polyclonal antibody to the salmonella effect protein SopB. According to the invention, the polyclonal antibody to the salmonella effect protein SopB is prepared by using the method, and the polyclonal antibody is beneficial for immunoblotting analysis and immunofluorescent staining laser confocal microscopic observation of the salmonella effect protein SopB and for in-depth research on the infection mechanism of enteropathogens.

The invention relates to a graphene field effect transistor array biosensor and a preparation method and a detection method thereof. The invention relates to a biosensor and a preparation method and adetection method thereof, aiming at solving the problem of relatively low accuracy in the detection process of the existing graphene field effect transistor array biosensor. The sensor comprises a silicon substrate, an oxidization layer, a first metal grid electrode, a second metal grid electrode, a gate insulating layer, a first graphene conductive channel layer, a second graphene conductive channel layer, a first group source, a leakage electrode, a second group source and a leakage electrode; the preparation method comprises the following steps: I. making a transistor structure; II. performing aldehyde treatment on the graphene surface; III. modifying a detection unit graphene surface to capture antibody molecules; and IV. closing the remaining active sites on the graphene surfaces ofa detection unit and a reference unit; the detection method comprises the following steps: soaking the sensor in a solution containing be-detected antigen, and detecting the voltage at the graphene dirac point of the detection unit and the reference unit, to obtain the absolute concentration of the to-be-detected substance solution, wherein a difference value corresponds to a standard working curve.

The invention relates to a method using liquid chromatography to prolong the service life of a separated biological substance and to reduce and lower the preparation time and requirements for a separated biological substance sample. According to the method, a series of chemical modifications are performed on the surface of a stationary phase, a bimolecular layer stabilized by a polymer is added to the surface of the stationary phase, and the bimolecular layer cannot generate nonspecific binding with biomass such as a protein, so that the risk that the stationary phase is polluted and absorbed by a complicated macromolecule protein is reduced greatly.

The invention discloses a kit and application thereof in detection of a vascular endothelial growth factor. The kit contains the following components which are independently stored: an antibody-coated magnetic bead working solution, a VEGF labeled antibody, a VEGF calibrator, a VEGF quality control product and a sample diluent. The antibody-coated magnetic bead working solution is a magnetic bead suspension coated with an antibody A. The VEGF labeled antibody is obtained by labeling an antibody B through an acridinium ester modifier. The acridinium ester modifier is acridinium sulfonyl hydrazide amine inner salt or other acridinium ester derivatives, and the antibody A and the antibody B are humanized VEGF antibody Fab segments with different amino acid sequences. The acridinium ester modifier is selected to be coupled with the humanized anti-VEGF antibody Fab segments, so that the stability is high, the sensitivity and the detection range of a detection system are improved, the storage time of the detection system is prolonged, and the reaction time is shortened. The linear range of the kit is 0.5-50000 pg / mL.

The present invention relates to a display device using a semiconductor light emitting element and a method for manufacturing the same, and more particularly, to a display device using a semiconductor light emitting element having a size of several [mu] m to dozens of [mu] m and a method for manufacturing the same. The present invention provides a display device, which is characterized by comprising: a base part; a plurality of transistors disposed on the base part; a plurality of semiconductor light-emitting elements disposed on the base part; a plurality of wiring electrodes disposed on the base part and electrically connected to the plurality of transistors and the semiconductor light emitting element; a partition wall disposed on the base part and formed so as to cover the plurality of transistors; and a connection electrode connecting a portion of the plurality of transistors and a part of the plurality of wiring electrodes, the connection electrode being formed so as to penetrate the partition wall.

The present disclosure provides a device for self-assembling semiconductor light-emitting diodes, in which the device includes an assembly chamber having a space for accommodating a fluid; a magneticfield forming part having magnets for applying a magnetic force to the semiconductor light-emitting diodes dispersed in the fluid and a horizontal moving part for changing positions of the magnet so that the semiconductor light-emitting diodes move in the fluid; a substrate chuck having a substrate support part configured to support a substrate, a vertical moving part for lowering the substrate sothat one surface of the substrate is in contact with the fluid in a state in which the substrate is supported, and an electrode connection part for applying power to the assembly electrode to generate an electric field so that the semiconductor light-emitting diodes are placed at predetermined positions of the substrate in a process of moving the semiconductor light-emitting diodes by a positionchange of the magnets; and a controller for controlling a movement of the magnetic field forming part and the substrate chuck, wherein the controller controls a depth at which the substrate is submerged in the fluid based on a degree of warping of the substrate.

The invention discloses a helicase-dependent isothermal DNA amplification-based method for detecting microRNA. The method comprises the following steps: (1) extracting total RNA in a sample; (2) adding a single-strand DNA probe and excessive exonuclease I into the extracted total RNA, incubating in a reacting buffer solution to realize specific combination of target microRNA and the single-strand DNA probe, and removing residual single-strand DNA probe by utilizing the excessive exonuclease I; and (3) adding upstream primer, downstream primer, single-strand binding protein and helicase to the reaction system, amplifying the target microRNA, and detecting expression of the target microRNA through a fluorescent signal. According to the method, the background is reduced by digesting the exonuclease I, and the signal is amplified by means of exonuclease assisted isothermal amplification (HDA) reaction, so that the microRNA can be quickly and sensitively detected.

The invention discloses a production technology for preparing an NC (Nitrocellulose Filter) membrane by lineation. The production technology comprises the following working procedures: operating membrane lineation in a continuous state, and drying after membrane lineation; after the working procedure of drying after membrane lineation is carried out, sealing a membrane which operates under a continuous state, and drying after sealing, wherein the operation time of the working procedure of drying after membrane lineation is 2-4 min, and the operation time of membrane sealing and working procedure of drying after sealing is 3-6 min. The NC membrane obtained by the technical scheme of the invention can be used for effectively avoiding the diffusion and the widening of an antibody line, the detection fluorescence of the antibody line is centralized, meanwhile, the specificity combination of the antibody line is improved so as to obviously lower the intra-batch differences and the inter-batch differences of production, and the accuracy and the repeatability of a detection result are improved.