Novel 2019 coronavirus antigen detection reagent and preparation method thereof
A coronavirus and antigen detection technology, applied in the field of analysis and detection, can solve the problems that antibody detection cannot be used as a diagnostic indicator alone, cannot exclude infection or explain the infection status, and antibody detection cannot be used alone to diagnose new coronavirus.
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Embodiment 1
[0064] Preparation of reagent strips:
[0065] 1. Solution preparation:
[0066] (1) Microsphere labeling buffer: 50mM MES, pH 6.0
[0067] (2) Microsphere blocking solution: 50mM ethanolamine solution + 0.1-5% BSA
[0068] (3) Microsphere storage solution: 50mM PBS+0.05-0.5%BSA+0.01-0.1%Tween20+0.02%sodium azide
[0069] (4) 10mg / ml NHS
[0070] (5) 10mg / ml EDC
[0071] (6) Glass fiber pretreatment solution: 50Mm borate buffer + 2-10% sucrose + 0.1-0.5% TW20 + 0.1-5% BSA
[0072] (7) Antibody coating solution: 10mM PBS buffer + 1-5% sucrose
[0073] 2. Glass fiber pretreatment
[0074] Soak the glass fiber membrane in the pretreatment solution for 30 minutes, take it out and dry it at room temperature for later use.
[0075] 3. Time-resolved Microsphere Labeling
[0076] 3.1 Prepare fresh EDC and Sulfo-NHS aqueous solutions respectively, with a concentration of 10 mg / mL. EDC and Sulfo-NHS are stored at low temperature and the reagents are susceptible to moisture, so th...
Embodiment 2
[0095] Repeat Example 1, the only difference is that the NC membrane is the NC membrane prepared according to the aforementioned specific method of the present invention.
[0096] After testing, compared with Example 1, when detecting SARS-CoV-2, the time of lateral wicking and absorption of the NC membrane of this example increased by about 22%, and the binding ability of the membrane to N protein increased by about 20%, The sensitivity can be increased by about 2.4 times. This clearly shows that the detection reagent of the present invention has more superior performance.
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