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Rapid noninvasive female corpus luteum function monitoring technology

An antibody and conjugate technology, applied in the field of non-invasive monitoring of female fertility, to reduce steric hindrance, avoid non-specific binding, and improve pregnancy success rate

Active Publication Date: 2016-11-23
NANJING JILANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, so far, there is no convenient and easy-to-use commercial in vitro noninvasive diagnostic product for infertility at home and abroad, which is used to quantitatively monitor the function of the corpus luteum in women, determine whether there is ovulation, whether pregnancy can occur after ovulation, and related female fertility issues. Force and Hormone Infertility

Method used

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  • Rapid noninvasive female corpus luteum function monitoring technology
  • Rapid noninvasive female corpus luteum function monitoring technology
  • Rapid noninvasive female corpus luteum function monitoring technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1: PdG-Linker-CO 2 Preparation of H(1)

[0068] PdG (0.1 mmol) was dissolved in DMF (0.5 mL), and a DMF solution (0.8 ml) of DCC (0.143 mmol) and NHS (0.143 mmol) was added. The reaction mixture was stirred for 7 hours, then a solution of 6-aminocaproic acid (0.119 mmol) in chloroform (1 mL) and triethylamine (0.5 mL) was added. The reaction was stirred overnight, and after removal of the solvent, the residue was chromatographed on silica gel with dichloromethane:methanol (8:2): the product PdG-Linker-CO 2 H (1; 0.025 mmol). Yield: 25%.

Embodiment 2

[0069] Embodiment 2: Preparation of PdG-Linker-OVA conjugate (1)

[0070] PdG-linker-CO 2 H (1; 0.05mmol) in a small test tube, add 0.2ml of the above DCC / DMF solution under stirring to dissolve, then add 0.2ml, and let it react at room temperature for 3 hours until a large amount of precipitation (urea is its by-product) . NaH 2 PO 4 .2H 2 O (0.1186g) and anhydrous Na 2 HPO 4 (0.46g) was dissolved in 20ml of water to prepare 0.2M phosphate buffer (pH7.4). At 4°C, OVA (44.34 mg, 1 μmol) was stirred and dissolved in the above 4 ml, 0.2 M phosphate buffer solution; the above DCC / NHS PdG solution (0.4 ml DMF solution) was dropped into the OVA solution under stirring. The coupling reaction of PdG-linker-OVA was stirred overnight at 4°C. The PdG-Linker-OVA conjugate was dialyzed against water 4 times (1.2 L each time) for 48 hours, and then dialyzed against phosphate buffer (1.2 L, pH7.4) for 24 hours. The purified PdG-Linker-OVA conjugate (1) was dialyzed and collected, f...

Embodiment 3

[0071] Embodiment 3: Preparation of PdG-Linker-OVA conjugate (2-4)

[0072] In a similar manner to Example 1 above, three portions of PdG (0.1 mmol) were dissolved in DMF (0.5 mL), and a DMF solution (0.8 ml) of DCC (0.143 mmol) and NHS (0.143 mmol) was added. The reaction mixture was stirred for 7 hours, then 7-aza-8-oxo-13-amino-tridecanoic acid, 7,14-diaza-8,15-dioxo-20-amino-tridecanoic acid, 7,14-diaza-8,15-dioxo-20-amino-di Decanoic acid and 4-oxo-5-aza-9,12,15-trioxa-18-amino-octadecanoic acid (0.119 mmol) in chloroform (1 mL) and triethylamine (0.5 mL) . The reaction was stirred overnight, and after removal of the solvent, the residue was chromatographed on silica gel with dichloromethane:methanol (8:2): the product PdG-Linker-CO 2 H (2-4; 0.023-0.026 mmol). The yields were: 23-26%, respectively.

[0073] With the method of above-mentioned embodiment 2, take above-mentioned PdG-linker-CO respectively 2 H (2-4; 0.05mmol) in a small test tube, add 0.2ml of the above...

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Abstract

The invention relates to a noninvasive female corpus luteum function monitoring technology, in particular to a full-quantitative or semi-quantitative rapid noninvasive urine test technology for determination of cyclic production amount of progesterone metabolites in women's urine so as to monitor and evaluate a female corpus luteum function, judge different types of ovulation cycle, determine whether ovulation is really performed or not and impregnation can be achieved or not after ovulation. According to the first implementation scheme, a protein hybridized couplet for determining pregnanediol glucuronide is related and contains pregnanediol glucuronide and a coupling protein. The protein hybridized couplet is characterized by further containing a junctional complex that connects the two parts. PdG of the hormone protein couplet is detected without an unsaturated labelled antibody, the sensitivity can be up to 0.1 ng / ml, and the linear range can be controlled to be 0.1-50 ng / ml.

Description

technical field [0001] The present invention relates to a non-invasive monitoring technology for female fertility, in particular, it relates to a fully quantitative or semi-quantitative rapid non-invasive urine testing technology to measure the periodic production of progesterone metabolites in female urine, so as to monitor and evaluate female luteal function , Judging different ovulation cycle types, determining whether ovulation is real and whether pregnancy can occur after ovulation. Background technique [0002] According to the 2012 "China Infertility Status Survey Report", the infertility rate in China is as high as 12.5%, and the female cause accounts for more than 50%. The number exceeds 50 million. Infertility treatment failure accounts for 66%, 98.9% of which are caused by not timely and accurate detection. Therefore, a non-invasive, cheap, and easy-to-use female luteal function monitoring technology is invented, which is especially suitable for the needs of Chi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765C07K14/77C07K14/795G01N33/68G01N33/58
CPCC07K14/765C07K14/77C07K14/795C07K19/00G01N33/58G01N33/581G01N33/582G01N33/689
Inventor 顾泳川吴银秋
Owner NANJING JILANG BIOTECH CO LTD
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