A graphene field effect transistor array biosensor and its preparation method and detection method

Abstract

The invention relates to a graphene field effect transistor array biosensor and a preparation method and a detection method thereof. The invention relates to a biosensor and a preparation method and adetection method thereof, aiming at solving the problem of relatively low accuracy in the detection process of the existing graphene field effect transistor array biosensor. The sensor comprises a silicon substrate, an oxidization layer, a first metal grid electrode, a second metal grid electrode, a gate insulating layer, a first graphene conductive channel layer, a second graphene conductive channel layer, a first group source, a leakage electrode, a second group source and a leakage electrode; the preparation method comprises the following steps: I. making a transistor structure; II. performing aldehyde treatment on the graphene surface; III. modifying a detection unit graphene surface to capture antibody molecules; and IV. closing the remaining active sites on the graphene surfaces ofa detection unit and a reference unit; the detection method comprises the following steps: soaking the sensor in a solution containing be-detected antigen, and detecting the voltage at the graphene dirac point of the detection unit and the reference unit, to obtain the absolute concentration of the to-be-detected substance solution, wherein a difference value corresponds to a standard working curve.

Description

technical field [0001] The invention relates to a biosensor, a preparation method and a detection method thereof. Background technique [0002] At present, the means of biological detection mainly include flow cytometer, PCR instrument, and immune kit. Flow cytometry detection and PCR detection require cell (or DNA) extraction, culture, and labeling, which not only takes a long period but also has huge equipment, which obviously cannot meet military needs. The detection of immunological kits requires a variety of reagents, and each reagent added in the process must be washed repeatedly, which requires high operating technology. In addition, the above methods all use fluorescent labeling and enzyme labeling detection. The luminescent functional groups of fluorescent dyes and enzymes are unstable and are easily affected by environmental factors. They have strict requirements on the operating environment and are not suitable for on-site environmental testing. Field-effect tra...

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Problems solved by technology

[0003] The object of the present invention is to provide a kind of graphene field effect transistor array biosensor and its preparation method and detection method; To solve the problem that the existing graphene field effect transistor biosensor all uses a standard blank sample as a control group in the detection process, Detect the concentration value of the relative blank sample, but it is impossible to separate the analyte in actual detection to obtain an ideal blank group, so the problem of low accuracy

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