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nc film and production process of scribing to prepare nc film

A production process and film-scribing technology, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of high coefficient of variation and low repeatability, and achieve the effects of reducing diffusion, improving accuracy, and avoiding antibody-antigen reactions

Active Publication Date: 2021-03-02
HEBEI TEWENTE BIOTECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Sensitivity and resolution are important indicators for the use of NC membranes. In addition to factors such as membrane material, membrane purity, and C / T line antibody specificity, they are also related to the manufacturing process of NC membranes. The existing NC membranes are produced using The process of constant temperature drying after the NC film is completely scribed. Since the NC film is produced in rolls and then cut and assembled, the NC film will be re-placed in the oven after the film is finished. During the drying process, there will be some antibody lines on the film. Different degrees of diffusion and widening lead to differences within the same batch and between batches, resulting in high coefficient of variation and low reproducibility of results. When used in large quantities, this difference will have a very large impact on the results. Impact

Method used

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  • nc film and production process of scribing to prepare nc film

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] This embodiment relates to the production of scribed NC film, comprising the following steps:

[0026] a. Filming and drying after filming:

[0027] a1. Film marking: Take the NC film roll and place it on the reel, and then pass through the film marking device to mark or spray the antibody solution diluted to the required concentration on the NC film;

[0028] a2. Drying after scribing: While scribing the back end of the NC film, the front end of the NC film that has been scribed immediately enters the drying tower that has been preheated to a drying temperature of 35-40°C in advance, that is, the scribing is running in a continuous state , Drying process after scribing film, wind up after drying or continue to carry out follow-up processing in continuous operation state;

[0029] The running time of the scribing and drying process after scribing is controlled within 2 to 4 minutes;

[0030] b. Membrane sealing and drying process after sealing

[0031] b1. Membrane s...

Embodiment 21

[0039] This example involves the verification of the performance of the reagent strips produced by the NC membrane produced by the process described in Example 1. The formula of the blocking solution used in the production process of the NC membrane is as follows: the blocking solution consists of PEG4000 as the sealing component, Surfynol 485 as the surfactant component, Pluranic L64 was prepared by dissolving in PB solution with pH 7.0 and 50mM and adjusting the pH of the blocking solution to 7.0; the mass percentages of PEG4000, Surfynol 485 and Pluranic L64 in the blocking solution were 0.5%, 0.6%, 0.2% respectively %.

[0040] The present embodiment and follow-up examples and comparative examples all take BNP reagent strips as an example (the detection range of different reagent strips is different, such as the detection range of BNP reagent strips is 6pg / ml~4000pg / ml, the detection range of cTnI reagent strips is 0.05ng / ml~30ng / ml), the performance experiment operation i...

Embodiment 22

[0047] This example relates to the influence of the composition of the blocking solution on the performance of the reagent strip.

[0048] The reagent strips were prepared by using the NC film obtained by the substantially same production process as in Example 2.1, and the performance of the reagent strips was tested (the test method was the same as in Example 2.1). The difference between this reagent strip and the reagent strip of Example 2.1 is that the blocking solution used in the production process is different, and the blocking solution consists of blocking component PEG4000, surfactant component Surfynol 485, dissolved in pH7.0, 50mM PB solution and adjust the pH of the blocking solution to 7.0; the mass percentages of PEG4000 and Surfynol 485 in the blocking solution are 0.5% and 0.6% respectively. The test results are shown in the table below:

[0049] Test items Test Results Accuracy: relative deviation 7％ Within-assay precision: coefficient of...

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Abstract

The invention discloses a production technology for preparing an NC (Nitrocellulose Filter) membrane by lineation. The production technology comprises the following working procedures: operating membrane lineation in a continuous state, and drying after membrane lineation; after the working procedure of drying after membrane lineation is carried out, sealing a membrane which operates under a continuous state, and drying after sealing, wherein the operation time of the working procedure of drying after membrane lineation is 2-4 min, and the operation time of membrane sealing and working procedure of drying after sealing is 3-6 min. The NC membrane obtained by the technical scheme of the invention can be used for effectively avoiding the diffusion and the widening of an antibody line, the detection fluorescence of the antibody line is centralized, meanwhile, the specificity combination of the antibody line is improved so as to obviously lower the intra-batch differences and the inter-batch differences of production, and the accuracy and the repeatability of a detection result are improved.

Description

technical field [0001] The invention relates to the technical field of NC film production, in particular to a production process for preparing NC film by scribing, and the NC film produced through the production process. Background technique [0002] Nitrocellulose filter membrane, referred to as NC membrane, is used as the carrier of quality control line and antibody line (ie C / T line) in colloidal gold test paper, and it is also the place where the immune reaction occurs. One of the most important consumables. NC membrane is generally made of raw material cellulose nitrate particles, which are dissolved to form a homogenate, and then the prepared homogenate is passed through the roller to form a thin film spread on a smooth flat carrier, and the forming agent in the homogenate is gradually volatilized and then dried and formed. . NC membrane has a strong binding ability to proteins, and is suitable for various color development methods, including isotope, chemiluminescen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531
CPCG01N33/531
Inventor 郭耀光牛海燕
Owner HEBEI TEWENTE BIOTECH DEV CO LTD
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