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Detection kit for monkey pox virus antigen and preparation method thereof

A technology for antigen detection and monkeypox virus, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of dependence on equipment, easy pollution, and no diagnostic methods have been established, so as to achieve strong anti-interference ability, improve detection accuracy, Effect of reducing the risk of stealth transmission

Pending Publication Date: 2022-07-15
山东康华生物医疗科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, biological polymerase chain reaction (PCR) is the main detection method for detecting monkeypox virus at home and abroad, but it needs to rely on equipment and is prone to pollution
At present, there is no other diagnostic method except PCR detection method established in our country

Method used

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  • Detection kit for monkey pox virus antigen and preparation method thereof
  • Detection kit for monkey pox virus antigen and preparation method thereof
  • Detection kit for monkey pox virus antigen and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] like Figure 1-3As shown, a monkeypox virus antigen detection kit includes a detection card and a sample extraction solution. The detection card includes an upper cover 13, a lower cover 14 and a monkeypox virus antigen detection test strip 3. The upper cover 13 and the lower cover 14 are mutually Buckle connection, a accommodating cavity is formed between the upper cover 13 and the lower cover 14, the monkeypox virus antigen detection test strip 3 is located in the accommodating cavity, and the monkeypox virus antigen detection test strip 3 includes a sample pad 5, a silicon core and a gold shell combined Pad 6, solid-phase antibody reaction membrane 7, absorbent paper 8 and PVC plate 4, the solid-phase antibody reaction membrane 7 is pasted on the PVC plate 4, and both ends of the solid-phase antibody reaction membrane 7 are respectively overlapped with silicon core gold shell binding pads One end of 6 and one end of the absorbent paper 8, the other end of the silicon...

Embodiment 2

[0058] A detection method of a monkeypox virus antigen detection kit comprises the following steps:

[0059] (1) Detection:

[0060] a. The test samples include oropharyngeal swabs, body fluids, blood and skin lesions, wherein the skin lesions include blister fluid, pustule fluid, and crusts;

[0061] b. Add 0.3mL of sample extraction solution to the attached reagent tube;

[0062] c. Oropharyngeal swabs: Wipe the base of the tongue and the tonsils on both sides with cotton swabs for at least three times, collect scabs with sterile tweezers, collect 200 μL of body fluids, blister fluid, and pustule fluid with a disposable register, and immerse the collected samples in the reagent The sample extraction solution in the tube is fully stirred and used as the sample solution to be tested;

[0063] d. Drop 80 μL of whole blood or the sample solution to be tested prepared in step c to the sample addition hole of the test card, and the sample solution to be tested reaches the test s...

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Abstract

The invention relates to a monkey pox virus antigen detection kit and a preparation method thereof, and belongs to the technical field of in vitro diagnostics, the monkey pox virus antigen detection kit comprises a detection card and a sample extracting solution, the detection card comprises an upper cover, a lower cover and a monkey pox virus antigen detection test strip, the monkey pox virus antigen detection test strip comprises a sample pad, a silicon core gold shell combination pad, a solid-phase antibody reaction membrane, absorbent paper and a PVC plate, a sample adding hole and an observation window are formed in the upper cover, and the preparation method of the monkey pox virus antigen detection kit comprises a preparation method of the silicon core gold shell combination pad, a preparation method of the sample pad and a preparation method of the solid-phase antibody reaction membrane. The kit can detect various samples including oropharynx swabs, body fluid, blood and skin focus tissues (including vesicular fluid, pustule fluid and scab), is high in sensitivity, and can effectively shorten the window period of virus diagnosis so as to reduce the hidden transmission risk caused by the incubation period and effectively control the large-scale transmission of epidemic situations.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to a monkeypox virus antigen detection kit and a preparation method thereof. Background technique [0002] Monkeypox is a zoonotic disease caused by the monkeypox virus (MPXV), which was first discovered in primeval forests in West and Central Africa. Monkeypox virus is similar to vaccinia virus and smallpox virus. The clinical manifestations are fever after 10-12 days of exposure, and skin rash after 2-3 days of fever. Changes in the rash, from maculopapular to small blisters, pustules, and crusting in about 10 days. The virus can be transmitted from animals to humans through direct and close contact, mainly through blood, body fluids, skin or mucous membrane wounds. Secondary human transmission is mainly through airborne droplets, which can infect the mucous membranes of the eyes, nose and throat. [0003] On May 21, 2022, the World Health Organization issued a monke...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/532G01N33/543
CPCG01N33/56983G01N33/532G01N33/54306G01N33/54346
Inventor 杨帆杨致亭杨明霞曹传美赵倩
Owner 山东康华生物医疗科技股份有限公司
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