Optical control system of two-photon fluorescence immunoassay analyzer

Abstract

The invention relates to the technical field of optical control systems, in particular to an optical control system of a two-photon fluorescence immunoassay analyzer. The system is characterized in that a two-photon laser generation module, a detection control module and an adjustment transmission module are mounted on a mounting bottom plate; a photomultiplier is arranged in the detection controlmodule; two-photon laser emitted by the two-photon laser generation module can be focused into a reaction cup through the detection control module and the adjustment transmission module; and the two-photon laser excites a detection substance in the reaction cup to generate fluorescence, and the fluorescence can return to the photomultiplier of the detection control module through the adjustment transmission module to be measured. The two-photon laser generation module is used for generating two photons, and due to the fact that the wavelength of the two photons is long, compared with short-wavelength light, the long-wavelength light is less affected by scattering and easily penetrates through a specimen, background interference fluorescence is further reduced, and the accuracy of equipment detection and analysis is improved.

Description

technical field [0001] The invention relates to the technical field of optical control systems, in particular to an optical control system for a two-photon fluorescence immunoassay analyzer. Background technique [0002] In general fluorescence phenomena, due to the low photon density of the excitation light, a fluorescent molecule can only absorb one photon at the same time, and then emit a fluorescent photon through a radiative transition, which is single-photon fluorescence. At present, the fluorescence immunoassay analyzers at home and abroad are based on single-photon fluorescence, and the single-photon fluorescence immunoassay analyzer has the following defects: because the single-photon wavelength is relatively short, it is greatly affected by scattering, and it is difficult to penetrate the specimen; single-photon excitation fluorescence background interference The strong fluorescence seriously affects the detection and collection of the fluorescence excited by the s...

AI Technical Summary

Problems solved by technology

At present, the fluorescence immunoassay analyzers at home and abroad are based on single-photon fluorescence, and the single-photon fluorescence immunoassay analyzer has the following defects: because the single-photon wavelength is relatively short, it is greatly affected by scattering, and it is difficult to penetrate the specimen; single-photon excitation fluorescence background interference The fluorescence is strong, which seriously affects the detection and collection of the fluorescence excited by the specimen itself, resulting in a decrease in the accuracy of the analysis results

[0003] In addition, the laser beam of the existing analyzer has the problem of inaccurate focus positioning

In the actual use process, it is impossible to accurately control the laser beam to make its directional and fixed-distance deflection move a small displacement, so as to achieve the purpose of setting the focus of the laser beam at the correct depth position of the liquid, resulting in a decrease in the accuracy and reliability of the analysis and detection results.

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