Preparation method of iridium complex and application in biphoton mitochondrial dyes
An iridium complex, mitochondrial technology, applied in the field of fluorescent dye research and development, can solve problems such as unfavorable mitochondrial morphology and changes, small Stokes displacement, unfavorable mitochondrial dynamic changes, etc., to achieve good water solubility and stability, less damage to cells, The effect of excellent fluorescence intensity
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Embodiment 1
[0032] The preparation of embodiment 1 iridium complex
[0033] 1. Preparation of iridium complexes:
[0034] The synthetic route of the iridium complex synthesized by the present invention is as follows figure 1 shown. On preparation method, as the productive rate of similar compound is basically in 50% (with IrCl 3 According to the traditional synthetic route (that is, the imidazophenanthroline ligand L is first synthesized, and then combined with [Ir 2 (L') 4 Cl 2 ] generates [Ir(L') 2 L] + ) optimization, the present invention has designed a new synthetic route (that is, first synthesize the precursor iridium complex Y0 of o-phenanthroline-5,6-dione, and then generate the target compound with cinnamaldehyde), and the yield (with IrCl 3 Total) reaches 80%, and the product is single, and it is easy to separate and purify, which significantly reduces the loss of precious metal iridium and the complexity of synthesis.
[0035] (1) The synthesis method of the precursor ...
Embodiment 2
[0045] Example 2 The study of iridium complexes as two-photon dyes in cell mitochondria
[0046] Human lung cancer cells A549 with logarithmic growth cycle were co-stained with nuclear dye DAPI, mitochondrial dye MitoTracker GreenFM, and iridium complex, washed 5 times with phosphate buffer solution, and imaged on a Nikon A1plus two-photon confocal microscope with a 25x objective lens (Apo LWD / 1.10 DIC N2), NDD mirror, excitation light 800nm, record fluorescence images under 420nm (blue), 515nm (green), 590nm (red) filters respectively ( figure 2 ). from figure 2 It can be seen that the distribution of the four iridium complexes synthesized by the present invention completely overlaps with the mitochondrial dyes, but does not overlap with the cell nucleus dyes, which proves that they are all cell mitochondria-specific dyes. Judging from the fluorescence intensity ratio ( F red / F Green ), when the commercial mitochondrial dye MitoTracker Green FM was added first and t...
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