Virus disaggregating agent and method for disaggregating virus antigen-antibody complex and detecting HCV (Hepatitis C Virus) antigen

Abstract

The invention provides a virus disaggregating agent and a method for disaggregating virus antigen-antibody complex and detecting HCV (Hepatitis C Virus) antigen. The virus disaggregating agent is characterized in that each 100ml of disaggregating agent comprises 0.1-1ml of detergent, 0-20g of protein denaturant, 0.01-1ml of reducing agent, 0.1-1ml of fat solvent, and base liquid as remainder amount. The antigen-antibody complex obtained by the neutralization reaction of testing blood sample or antiserum and virus antigen is disaggregated into free components, or virus core antigen is fully exposed and the antigen reactivity of the virus core antigen is remained, thereby remarkably improving the sensitivity by comparing with the prior HCV core antigen detection method; in addition, the detection window period of the antigen is 49 days shorter than that of the anti-HCV antibody averagely, the situation appears within 1-2d after HCV-RNA appears, thereby efficiently shortening the window period of the blood serum before transformation, which is of important signification on improving the detection rate of affected person in the window period and the safety of blood transfusion and blood products. In the invention, the virus disaggregating agent is suitable for the disaggregation of common virus antigen-antibody complex including HCV and HAV (Hepatitis A Virus).

Description

technical field [0001] The invention relates to a virus lysing agent and a method for using the lysing agent to lyse a virus antigen-antibody complex for rapid and accurate detection of hepatitis C virus HCV antigen, which belongs to the field of biotechnology. Background technique [0002] Hepatitis C virus HCV is a hepatotropic chronic virus. Except for some (about 10%-20%) of HCV-infected patients who can heal themselves, most of them will turn into chronic infection. The natural course of the disease is as long as 20-30 years, and about 20% of them are chronically infected. It will develop into liver cirrhosis, and 20% of infected patients will develop into hepatocellular carcinoma. The incidence of chronicity is significantly higher than that of hepatitis B, and the mortality rate is higher. Therefore, the detection of hepatitis C virus HCV is of great significance to the early diagnosis of hepatitis C virus infection and the guidance of clinical treatment. Existing d...

AI Technical Summary

Problems solved by technology

In practical application, since the free core antigen in the serum is very small, and the core antigen is wrapped by the envelope protein, at the same time, the serum may contain one or more different antigens, and some antigens are not easy to detect because the content is too low , thus affecting the detection of antigens, which is an important reason for the low detection rate and low positive rate of the existing antigen detection methods

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