The invention relates to the field of biomedical clinical diagnosis, and specifically relates to a kit and a detection method for rapid quantitative detections of hepatitis virus nucleic acids. The kit comprises a nucleic acid releasing reagent, a nucleic acid amplification reagent, a control substance, a standard substance, an internal standard, and a template. The nucleic acid releasing reagent is a water solution of SDS, 3-[3-(cholamidopropyl)dimethylammonio]propanesulfonate (Chaps), (NH4)2SO4, and formamide. According to volume percentages, the nucleic acid releasing reagent comprises 0.25% of SDS, 0.25% of Chaps, 5% of (NH4)2SO4, 1% of formamide, and balance of water. The invention has the advantage that the invention provides a releasing reagent which can highly efficiently and rapidly release hepatitis virus nucleic acid, and an application thereof. Under the effect of the nucleic acid releasing reagent, the nucleic acid can be directly released from trace samples, and can be directly added into a PCR reaction liquid for fluorescent quantitative detection. Therefore, rapid nucleic acid detection is realized. Sample nucleic acid is not required to be extracted independently. Nucleic acid extraction and fluorescence quantitative PCR detection are directly achieved in a PCR reaction tube.