Gene detection method for communicating hydrocephalus

Abstract

The invention discloses a gene detection method for communicating hydrocephalus. According to the method, a venous blood sample of a to-be-tested patient is directly used as an analysis sample. The method comprises the following steps: (1) extracting RNA; (2) performing fluorescent PCR (polymerase chain reaction) amplification; (3) performing fluorescent amplification; and (4) performing result analysis. The detection method disclosed by the invention is simple to sample, is high in sensitivity and strong in specificity, can be used for early diagnosis, and is of great significance for carrying out early warning on communicating hydrocephalus patients and taking effective treatment means.

Description

technical field [0001] The invention relates to a detection method, in particular to a gene detection method for communicating hydrocephalus. Background technique [0002] Hydrocephalus is divided into communicating hydrocephalus and non-communicating hydrocephalus. The formation and absorption of cerebrospinal fluid in normal people is a dynamic balance process. The formation of cerebrospinal fluid is produced by the choroid plexus of the lateral ventricle. The cerebrospinal fluid produced by the choroid plexus passes through other ventricles, flows to the subarachnoid space through the space between the cerebellum and pons, and reabsorbs through the blood vessels in the subarachnoid space to achieve balance. If there is too much cerebrospinal fluid produced by the choroid plexus or fibrosis occurs in the subarachnoid space, it will lead to communicating hydrocephalus. Children with congenital communicating hydrocephalus have no obvious clinical manifestations at fi...

AI Technical Summary

Problems solved by technology

If there is too much cerebrospinal fluid produced by the choroid plexus or fibrosis occurs in the subarachnoid space, it will lead to communicating hydrocephalus. Children with congenital communicating hydrocephalus have no obvious clinical manifestations at first. The child showed obvious head circumference increase, language cognitive ability was affected, and limbs were paralyzed; the child needed a cerebrospinal fluid shunt operation, that is, the excessive cerebrospinal fluid was shunted from the lateral ventricle to the abdominal cavity for treatment through a catheter; therefore, the patient’s Early diagnosis is very important, which can achieve early diagnosis and early treatment; the current diagnosis is mainly based on MRI scanning technology. Through scanning the patient's brain, the Evan ratio is determined, that is, the ratio of the widest part of the lateral ventricle to the widest part of the brain. If it is greater than 0.3, it is hydrocephalus, but the cost of MRI scanning is high, it is sensitive to the patient's body movement, it is easy to produce artifacts, the scanning time is long, and some patients will have claustrophobia; and when the MRI scan is used to judge Hydrocephalus can only be diagnosed after the patient becomes ill or even seriously ill, which is not conducive to the control of the disease. If the patient with hydrocephalus is diagnosed and treated early, the disease can be effectively controlled. Low cost, high sensitivity, strong specificity, in vitro gene detection method for early diagnosis of communicating hydrocephalus, which is of great significance for early warning and effective treatment of communicating hydrocephalus patients