68results about How to "Sampling is simple and convenient" patented technology

The invention relates to an intelligent injection molding machine and an injection molding method thereof. The intelligent injection molding machine comprises an input unit, a logic operation unit, a storage unit, a detection unit and an executing unit, wherein the detection unit comprises a position sensor, a pressure sensor, a strain sensor and a temperature sensor; the logic operation unit acquires information of speed, pressure, temperature and strain in each moving process detected by the detection unit and carries out operation and comparison to generate curves of speed, pressure, temperature and strain; the logic operation unit generates a corrected multi-section grading injection-molding process according to the information and stores the corrected multi-section grading injection-molding process in the storage unit; the injection molding machine acts according to the corrected multi-section grading injection-molding process. The sectional injection process obtained by turning-point analysis has an accurate sectional effect, i.e., the sectional process conforms to the actual needs of product molding, so that the sectional injection process has the process characteristic of stable molding.

The detection process of SARS virus of the gene detection reagent kit with rationing standard specific SARS virus nucleic acid as reference includes RNA extraction, PCR amplification and fluorescent detection. The venous blood sample, gargled liquid or respiratory tract secretion of the patient is used as the analysis sample directly, RNA of the sample is extracted as the nucleic acid template, and the template is used in fluorescent PCR amplification. The used fluorescent amplification detecting reagents includes one-step process RT-PCR buffering liquid, deoxynucleoside triphosphate (dNTP) mixture, specific amplification primer and specific probe. The method of the present invention is fast and convenient in detecting SARS virus.

The invention relates to a field sampling device special for transformer insulating oil. The device comprises a core piston, a circular cover body, the central hole thereof is sleeved on the core piston, and a circular cylinder body which is matched with the circular cover body. A cylindrical core piston handle is screwed at the left end of the core piston. A core piston head matching with the inner diameter of the circular cylinder body is fixed at the right end of the core piston. A plurality of evenly-spaced capacity calibration loops are fixed at the surface of the middle part of the core piston. An air-exchange hole running through the circular cover body is arranged in the top surface of the circular cover body. Groove skirt edges with direction to the right are arranged at the periphery of the circular cover body. A pipeline communicated with the interior of the circular cylinder body is arranged at the right end of the upper sidewall of the circular cylinder body. An exhaust and oil discharge valve is arranged on the pipeline. A pipeline communicated with the interior of the circular cylinder body is arranged at the bottom surface of the right side of the circular cylinder body. An oil pumping valve is arranged on the pipeline. The sampling device avoids the contact of oil and air, can fix the volume in a container after quantitative sampling, avoids overpressure or cavatition in the container, and reduces sampling errors.

The invention provides a recombinant protein and test paper for detecting a novel coronavirus 2019-nCoV IgA antibody, and a preparation method and application, and belongs to the technical field of virus detection. The amino acid sequence of the recombinant protein is shown in SEQ ID No.1; the test paper comprises a bottom plate as well as a sample absorption pad, a fluorescent microsphere pad, anitrocellulose membrane and a water absorption pad which are in sequential lap joint and adhesion to the bottom plate; a 2019 novel coronavirus specific antigen marked by fluorescent microspheres is sprayed to the fluorescent microsphere pad; a detection zone and a quality control zone are fixed on the nitrocellulose membrane; an anti-human IgA antibody is sprayed to the detection zone; and a sheep anti-chicken IgY antibody is sprayed to the quality control zone. By detecting the IgA antibody in a saliva sample, the test paper provided by the invention is capable of simply, rapidly and accurate detecting novel coronaviruses, and early-stage detection on the novel coronaviruses can be achieved.

The invention discloses a computer technology-based water quality detection device convenient for sampling. The water quality detection device comprises a detection water tank; a water inlet pipe anda water outlet pipe are mounted at the top and the bottom of the outer wall of one side of the detection water tank through bolts respectively; a first water suction pump is mounted on the outer wallof one end, far away from the detection water tank, of the water inlet pipe through bolts; the water inlet end of the first water suction pump is connected with a diversion valve through a water pipe;diversion pipes distributed equidistantly are mounted on the outer wall of one end, far away from the first water suction pump, of the diversion valve through bolts; and electromagnetic valves are mounted on the outer walls of the circumferences of the diversion pipes through bolts. A pH meter is used for detecting a pH value of sampled water; a turbidity detector is used for detecting the turbidity of the sampled water; a water quality ion detector is used for detecting the ion concentration of the sampled water; a water quality hardness detector is used for detecting the hardness of the sampled water; and detection data is connected with a computer through a signal line to perform analysis and detection, so that the precision and accuracy of water quality detection are improved.

The invention discloses a sealant dispersing system with a circulating water temperature adjusting device. The system comprises a cylindrical main container body and further comprises a vertical stirring shaft, a stirring knife, a vertical stirring motor, a rotary connector, a sealant pumping pump, a transfer container, a horizontal stirring shaft, a stirring blade, a horizontal stirring motor, a stirring knife lifting mechanism, a sampling mechanism and a circulating water temperature adjusting device. By means of the system, raw materials in the main container body can be more rapidly and more evenly stirred, so that raw materials on the upper, lower, side and middle portions in the container body are evenly dispersed more synchronously, dispersing operation time can be shortened, and production efficiency can be improved; in addition, in the dispersing treatment process, the temperature of sealant can be adjusted through the circulating water temperature adjusting device, and dispersing operation efficiency is further improved.

The invention provides an indoor simulation system for a sea ice freezing and thawing process experiment and relates to a device capable of simulating the forming and thawing processes of sea ice on the surface layer of an ocean in the natural environment in a laboratory. The indoor simulation system for the sea ice freezing and thawing process experiment is provided with a refrigerator, a semiconductor refrigerating device, a water storage tank, sampling ports, heat preservation layers and temperature probes; an air-cooling refrigerator is adopted to serve as the refrigerator, and a hole is formed in the top of the refrigerator; the upper portion of the water storage tank used for containing sea water is provided with an opening and an upper cover; the semiconductor refrigerating device is embedded in a top plate of the water storage tank, and a refrigerating piece of the semiconductor refrigerating device directly makes contact with air in the water storage tank; the periphery and bottom of a tank body of the water storage tank are wrapped with the heat preservation layers correspondingly so that sea ice can be prevented from being formed on the periphery and bottom of the tank body of the water storage tank; the sampling ports are formed in the side face of the tank body of the water storage tank and used for sampling air samples and water samples, and the sampling ports are sealed through water seal clamps; a drainage hole is formed in the bottom of the tank body of the water storage tank, and the drainage hole is externally connected with a vacuum bag; and the temperature probes are arranged on the periphery and bottom of the tank body of the water storage tank, and a balloon arranged on the upper portion of the tank body of the water storage tank communicates with the outside of the tank body of the water storage tank.

The invention discloses a peripheral blood transcription gene marker for breast cancer detection. The peripheral blood transcription gene marker comprises blood cell characteristic gene sequences of breast cancers shown in SEQ ID NO.1-SEQ ID NO.10. The invention also discloses application of the gene marker in preparation of breast cancer detection products. According to the peripheral blood transcription gene marker, early screening detection is carried out on the breast cancers through combinatory analysis on the expression conditions of transcription gene markers related to the breast cancers in peripheral blood. The peripheral blood transcription gene marker has the advantages of high sensitivity and strong specificity, the peripheral blood which is most easily collected clinically is taken as a detection sample, sampling is safe, simple and convenient, the peripheral blood transcription gene marker is very suitable for early screening and auxiliary diagnosis of the breast cancers of a large-scale population, and the application prospect is wide.

The invention discloses a method for measuring chemical components of liquid sodium silicate. The method comprises the following steps: (1) setting a constant value of a liquid sodium silicate standard sample; (2) preparing the liquid sodium silicate sample and determining the sampling amount; (3) drawing a working curve by employing an X-ray fluorescence spectrophotometer; (4) measuring contents of Na2O, SiO2, K2O and S in the liquid sodium silicate sample. The method has the characteristics of high rapidness and accuracy and simple operation, the sample is not required to be subjected to special treatment and can be directly subjected to sample injection and analysis, and various types of sodium silicate can be well measured; moreover, by employing the method, the application range of the X-ray fluorescent spectrometry is widened, and compared with the traditional wet process chemical analysis, the method has the advantages that sampling is simple and convenient, analysis speed is fast, the precision and the accuracy are high, the analytical testing efficiency is greatly improved, and the detection cost is saved.

The invention discloses a testing method for microsatellite instability of blood ctDNA. The method comprises the steps of performing probe capturing on the cfDNA which comprises ctDNA, and establishing a sequencing libray and PGM sequence according to an acquired target product; performing partitioning, quality controlling, filtering and comparing on generated data; then performing microsatelliteloci sequence extraction through a perl program, and comparing with a normal microsatellite sequence, detecting an abrupt change condition of the microsatellite loci and recording the abrupt change type, and finally evaluating the integral microsatellite stability state of the sample according to statistics of the stability of each microsatellite loci. The method can effectively and accurately test the microsatellite instability state of the sample and furthermore can test the abrupt change condition of the microsatellite instability loci. Compared with a traditional PCR testing method, the testing method according to the invention has advantages of higher speed, higher convenience, lower cost and more comprehensive testing.

The present invention provides a kind of measles virus fluorescence amplification detection kit and method for detecting measles virus by using said kit. Said kit includes measles virus hemagglutinin gene standard product, fluorescence amplification detection reagent and DNA polymerase and reverse transcriplase. The fluorescence amplification detection reagent mainly includes one-step method RT-PCR buffer fluid, specific amplification primer and specific probe and deoxynucleoside triphosphate mixture. Said method can directly detect pathogen nucleic acid, and can detect the specific gene containing measles virus.

The invention discloses a detachable coal seam gas coal core sampling machine. The sampling machine comprises a bottom plate, two walking positioning devices and a sampling device; the two walking positioning devices are arranged on the bottom plate; the two walking positioning devices are symmetrically mounted on the left side and the right side of the lower end of the bottom plate; an opening structure is formed in the middle of the right side of the upper end of the bottom plate; the sampling device is mounted on the left side of the upper end of the bottom plate; and the right side of thesampling device is located in the opening structure. According to the sampling machine, the difficult problems that during underground coal seam gas sampling, an existing sampling machine is inconvenient to move, poor in positioning effect, inaccurate in coal seam sampling, complex in sampling, inconvenient in operation, low in automation degree and low in working efficiency, and after sampling, the existing sampling machine cannot quickly take out coal cores in the sampling machine and effectively distinguish the coal cores, and is complex in operation, high in labor intensity and low in efficiency, and the like can be solved, so that the function of automatic quick sampling of underground coal seam can be realized.

The present invention provides a kind of H5N1-type avian influenza virus fluorescence amplification detection kit and detection method for detecting avian influenza virus byusing said kit. Said kit includes the following several portions: H5N1-type avian influenza virus hemagglutinin gene standard product, fluorescence amplification detection reagent and DNA polymerase and reverse transcriptase. The fluorescence amplification detection reagent mainly includes one-step method RT-PCR buffer fluid, specific amplification primer and specific probe and deoxynucleoside triphosphate mixture. Said method can directly detect pathogen nucleic acid, and can detect specific gene containing H5N1-type avian influenza virus.

The invention provides a rapid kit and a method for detecting genes containing 89k pathogenicity island Streptococcus type II through a Taqman-MGB double fluorescent probe PCR. The specific primer and the specific probe of the kit include a 16S upstream primer of 5'- AGCGCAGGCGGTTTGA-3', a 16S downstream primer of 5'-ACAGTTTCCAA AGCGTACTATGGTTA-3', a 16S specific probe of 5'-FAM-AGTCTG AAGTAAAAGGC-MGB-3', a 89K upstream primer of 5'-ATAAAAATACCGCT GTTGGATGGA-3', a 89K downstream primer of : 5'-CGTCTACACCGAATT GTT TTGCA-3', and a 89K specific probe of 5'-HEX- CATGGGAGATATAAAG A AC -MGB-3', wherein FAM and HEX are reporting fluorescent groups, and HGB is a decorative group. The method provided by the invention has easy and convenient sampling, rapid evaluation, high sensitivity and accurate results, and can be applied to early diagnosis of SS2 onset.

The invention discloses a sampling method of rock mass with weak structure surfaces. The method comprises the following steps: selecting a sampling part with weak structure surfaces and chiseling the sampling part to form a plane; pre-marking a sample area on the sampling plane, wherein hard rock layers at the upper part and the lower part of a weak structure layer are equal in thickness; paving steel wires on the surface of the sample area and coating a protection layer; cutting inwards by taking the edge of the protection layer as a base line vertical rock wall to form a side surface and a back surface of the sample, and protecting all the surfaces; curing the cut samples on the site and grinding the samples to obtain samples which meet the size requirement; chiseling the protection layer of the weak structure layer, sealing and filling the samples and enabling the samples to maintain the water content on the sampling site; then transporting the samples to a laboratory, cutting off the steel wires paved on the surfaces, enabling the weak structure layer to be exposed, and filling the samples in a cutting box for carrying out experiment. The method is capable of simply and conveniently obtaining disturbance-free complete rock samples with the same water content of the site and can be used for directly detecting basic rheological properties of the rock indoors.

The invention belongs to the field of biomedicines, relates to a colorectal cancer detection kit, and more particularly relates to a colorectal cancer detection kit taking excrement as a detection sample. The detection kit is characterized in that an Alu detection reagent and PvPP are contained. Through multi-time exploration in numerous colorectal cancer markers, Alu is discovered that the sample can be directly diluted for PCR amplification detection without going through complex steps of cell disruption, DNA extraction and the like on the premise of proper simple pretreatment, and good sensitivity and specificity are obtained.

The invention discloses a CDKN2A gene fragment and application of the primers of CDKN2A gene fragment in diagnosing tumors.By designing an upstream and downstream primer pair, the specific CDKN2A gene fragment is determined, the gene fragment shows remarkable high DNA methylation in tumor patients while showing low DNA methylation in normal people, and statistic differences exist between DNA methylation of tumor patients and that in normal people.Occurrence and development of tumors are tracked with the level changes of DNA methylation of the gene fragment, and thus a novel biomarker is provided for diagnosis, treatment effect monitoring and prognosis improvement judgment of tumors.

The invention discloses a soil sample storage device for geological exploration. The soil sample storage device comprises a cylinder body, a storage cylinder and a base, wherein four storage pipes arefixedly connected to the interior of the storage cylinder, and sampling mechanisms are arranged at the bottoms of the storage pipes; a moving mechanism is arranged in the base; a rotating mechanism is arranged in the cylinder body and is positioned at the periphery of the storage cylinder; and one end of an output shaft of a motor is fixedly connected with a rotating shaft through a coupler. Theinvention relates to the technical field of geological exploration. According to the soil sample storage device for geological exploration, the rotating shaft is driven to rotate through the motor, the rotating shaft and a rotary shaft drive a pinion to rotate, and the pinion drives a gear ring and the storage cylinder to rotate, so that discharging pipes at the bottoms of the storage pipes for storing different soil samples can be conveniently rotated to a discharging opening, then the discharging pipe corresponding to the discharging opening is opened, and the corresponding soil sample can be taken out; and the sampling is simpler and more convenient, and time-saving and labor-saving, and the detection efficiency is improved.

A layered water intake device includes a water intake device body (5); the lower part of an inner cavity of the water intake device body (5) is provided with an anti-floating counterweight body (6), and the upper part of the inner cavity of the water intake device body (5) is provided with limiting clamps (4); the inner side of a seal port of the water intake device body (5) is provided with a fixed sealing clamp (3-2); the upward side of the limiting clamps (4) of the inner cavity of the water intake device body (5) is provided with a mobile sealing clamp (3-1) which can move upper and down; the mobile sealing clamp (3-1) and a movable shaft (2) are connected with each other or are formed as a whole; one end of a descending measuring rope (1-1) is connected with the movable shaft (2); one end of one lifting measuring rope (1-2) and one end of the other lifting measuring rope (1-2) are respectively connected with two lugs outside the upper part of the water intake device body (5). The layered water intake device has the advantages that when used for layered water intake of river, rivus, lake and sea water bodies, the layered water intake device can perform continuously layered water intake at a same water intake plane position according to different depths, and also can effectively guarantee the accuracy of fetched water samples; and when layered water sample fetching is carried out in exploratory boring holes or hydrological water wells, the layered water intake device has no special requirements on hole wells, is convenient and concise to sample and higher in efficiency, and has wide application value.

The invention relates to the technical field of hydraulic engineering, and discloses a riverbed permeability coefficient testing device. The device comprises a sampling unit and a leakage unit, the sampling unit comprises a sampling pipe and a piston assembly; the bottom end of the sampling pipe is open, so that a riverbed soil sample enters the sampling pipe; the piston assembly is detachably inserted into the sampling pipe in a sealed mode and can reciprocate in the axial direction of the sampling pipe relative to the sampling pipe, the sampling unit further comprises a bottom end cover detachably arranged at the bottom end of the sampling pipe in a covering mode, and the bottom end cover is arranged to allow water to pass and prevent a riverbed soil sample from passing; the leakage unitcomprises a water seepage barrel, and the water seepage barrel is arranged to allow the sampling unit to be vertically inserted for a penetration test. The riverbed undisturbed soil sample can be conveniently and rapidly collected through the sampling unit, the sampling efficiency is greatly improved, interference of river water flow can be eliminated through the seepage unit for a seepage test,the flexibility and operability of the test are improved, and the test efficiency is improved.

The invention discloses a pipeline bio-membrane reactor with a detachable inner body. The pipeline bio-membrane reactor comprises a shell, the inner body and two double-layer water distributors, wherein the shell comprises a shell casing pipe and a connector, the inner body comprises an annular pipe section and a clack-shaped pipe section, the two double-layer water distributors are respectively arranged at the two ends of the inner body, and the inner body and the two double-layer water distributors are exactly right nested in the shell. The pipeline bio-membrane reactor is suitable for research of pipeline biological membranes in water supply pipe networks, uniform in water distribution and controllable in hydraulic conditions; after the biological membranes are cultured, the sampling and research of the biological membranes are carried out in the detachable inner body; the pipeline bio-membrane reactor is simple, convenient, fast, compact in structure and low in cost.

The purpose of the invention is providing a closed sampling method of a lithium ion battery electrolyte. A closed sampler for realizing the method comprises rapid joints, a sampler ball vale and a bottle body, the upper portion of the bottle body is provided with the sampler ball valve, and two sides of the sampler ball valve are provided with the rapid joints. Compared with original open sampling, the closed sampling method has the following obvious advantages: waste of a large amount of a material is avoided, and problems of discharging and flushing of the closed sampler are solved; the material floes in a closed state in the operating process, so a sample is not interfered by extraneous environment factors, and the quality of the sample is guaranteed; and the rapidly joints are respectively connected with an apparatus to be sampled and a sampling bottle, and the sampler ball valve is opened and closed to sample, so sampling is simple and convenient, and is convenient to operate.

The invention relates to a dry sand sampling drill. An internal drill bit is connected with an internal drill handle, an external drill bit is connected with an external drill handle, the internal drill bit is inserted into the external drill bit and is provided with a baffle, one end of the baffle is connected with the internal drill bit, and the other end of the baffle penetrates through the internal drill bit to extend out via a rope. The external drill bit of the external drill is inserted into sand, and the sliding collapse of walls of sand flow holes can be avoided due to the external drill; and the inner wall of the internal drill is a hollow cavity, the movable baffle is arranged in the cavity, and when the external drill is inserted into the sand, the internal drill is inserted into the external drill, the movable baffle is opened and is then closed through pulling the rope when drilling is carried out to a certain depth, and thus the sand can be removed. Therefore, the sampling is convenient and simple, and labor can be saved.

The invention discloses a time resolution immunity fluorescence kit for detecting the content of beta-hCG. The time resolution immunity fluorescence kit comprises a box body and a box cover which is arranged above the box body, wherein a separation layer which is used for placing an elisa plate coated by a beta-hCG monoclonal antibody is arranged in the box body; vessel holes for arranging reagent bottles are formed in the box body, and in the vessel holes, a reagent bottle with analysis solution, a reagent bottle with a cleaning solution, a reagent bottle with an enhanced solution, a reagent bottle with a beta-hCG correction product and a reagent bottle with a labeled anti-alpha-TSH monoclonal antibody are placed. In addition, the invention also discloses a preparation method of the kit and application of the kit in detecting the content of beta-hCG. The kit is high in sensitivity, wide in detection range and good in stability. The time resolution immunity fluorescence kit can be full-automatically operated by utilizing a full-automatic time resolution fluorescent immunity analyzer and is simple and time-saving to operate and high in sensitivity reaching up to 0.01mIU / ml, good in accuracy and free from radiation pollution.

The invention discloses a genetic detection kit for communicating hydrocephalus. The kit comprises a RT-PCR buffer, a deoxy nucleoside triphosphate mixture, specific amplification primers and probes. The kit is convenient to sample, and venous blood samples of a to-be-detected patient can be sampled; the fluorescence amplification detection kit has the advantages of high sensitivity and strong specificity and can be used for early diagnosis; and the kit is of great significance for giving the early warning of patients with communicating hydrocephalus and taking effective treatment means.

The invention discloses a gene detection method for communicating hydrocephalus. According to the method, a venous blood sample of a to-be-tested patient is directly used as an analysis sample. The method comprises the following steps: (1) extracting RNA; (2) performing fluorescent PCR (polymerase chain reaction) amplification; (3) performing fluorescent amplification; and (4) performing result analysis. The detection method disclosed by the invention is simple to sample, is high in sensitivity and strong in specificity, can be used for early diagnosis, and is of great significance for carrying out early warning on communicating hydrocephalus patients and taking effective treatment means.

The invention discloses a chemical reactor. The chemical reactor comprises a tank body, wherein a feed port, a tank cover, a pressure meter, a spray nozzle and a filter device are arranged on the tank body; a discharge port is formed in the lower part of the tank body; a material reaction cavity and a liquid storage cavity are formed in the tank body and are divided by a closing plate; a round hole for accommodating the filter device is formed in the upper part of the closing plate, the filter device is detachably arranged in the round hole, and a through hole for accommodating the spray nozzle is formed in the closing plate; a liquid pump is arranged in the liquid storage cavity, a liquid pipe is connected between the liquid pump and the spray nozzle, and a control box for controlling the liquid box to work is arranged on the liquid pump; a pressure induction line is arranged on the bottom surface of the reaction cavity and intelligently connected with the control box. With the adoption of the chemical reactor, the interior of the tank body can be washed rapidly in time, a liquid material is prevented from caking and sticking to the inside of the tank body, a conveying pipeline can be effectively prevented from being blocked, and further, whether a reaction in the reactor is completely finished can be conveniently detected.