Colorectal cancer detection kit

Abstract

The invention belongs to the field of biomedicines, relates to a colorectal cancer detection kit, and more particularly relates to a colorectal cancer detection kit taking excrement as a detection sample. The detection kit is characterized in that an Alu detection reagent and PvPP are contained. Through multi-time exploration in numerous colorectal cancer markers, Alu is discovered that the sample can be directly diluted for PCR amplification detection without going through complex steps of cell disruption, DNA extraction and the like on the premise of proper simple pretreatment, and good sensitivity and specificity are obtained.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a colorectal cancer detection kit, in particular to a simple colorectal cancer detection kit using feces as a detection sample. Background technique [0002] There are two main techniques commonly used in colorectal cancer screening. [0003] One is a fecal occult blood test. Usually, the occurrence and evolution of bowel cancer can occur without any symptoms in the early stage. Cancer cells can grow on the wall of the large intestine for decades and then metastasize to other parts. discharge. The fecal occult blood test is to detect the blood components in the stool (the detection object is hemoglobin). If multiple and continuous positive reactions indicate gastrointestinal bleeding, further examination should be done to guard against the occurrence of intestinal tumors. The advantage of this method is that the results are displayed quickly and clearly, and the results can be semi-qua...

AI Technical Summary

Problems solved by technology

But it has a series of disadvantages: First, its specificity is poor and it is greatly affected by diet

However, it also has a series of disadvantages: First, it requires pre-examination preparations. For colonoscopy, it is necessary to start eating a liquid or semi-liquid diet with less residue 3 days before the examination, fasting on the morning of the examination, and taking mannitol the day before the examination, etc. A cathartic to cleanse the bowel, usually taken with up to 2L of fluid, a cleansing enema to keep the bowel clean, after which no food can be given

[0008] However, due to the complex composition of feces, it cannot be directly amplified by PCR

The usual way to detect human nucleic acid in feces is to use the method of nucleic acid extraction. First, use mechanical force to fully mix and fragment the feces, and then use organic solvents or magnetic beads to capture all or the required DNA. The nucleic acid is enriched and then purified before performing PCR amplification experiments. The steps are cumbersome and time-consuming.

Images