566 results about "Cell disruption" patented technology

An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

The invention discloses a preparation method of a conductive graphene nanofiber membrane. The preparation method comprises the following steps: preparing an electrospinning nanofiber membrane by adopting electrostatic spinning equipment; performing ultrasonication on oxidized graphene by adopting ultrasound equipment and a cell disruption instrument, performing suction filtration on the electrospinning nanofiber membrane, and drying; performing reduction on the prepared conductive oxidized graphene nanofiber membrane by using a reducing agent, and then drying, so as to obtain the conductive graphene nanofiber membrane. According to the preparation method of the conductive graphene nanofiber membrane, provided by the invention, oxidized graphene is used for preparing the conductive oxidized graphene nanofiber membrane, and then the conductive oxidized graphene nanofiber membrane is subjected to reduction to prepare the conductive graphene nanofiber membrane with enhanced electrical conductivity.

The invention relates to an extraction method of spirulina phycocyanin. The method comprises the following steps of: processing spirulina powder suspension by adopting a freeze thaw method and a machine crushing process to obtain spirulina cell disruption liquid; performing fractional salting out to precipitate the spirulina cell disruption liquid with 30 percent ammonium sulfate liquid and 50 percent ammonium sulfate liquid to obtain phycocyanin coarse extract liquid 1; adding PEG 20000 (polyethylene glycol 20000) and NaCl to further precipitate out phycocyanin coarse extract with higher purity; dissolving the phycocyanin coarse extract with phosphate buffer to prepare phycocyanin coarse extract liquid 2; and extracting the phycocyanin coarse extract liquid with PEG 20000 / Na2SO4 aqueous two-water-phase system, and desalting extracted lower phase by dialysis to obtain phycocyanin fine extract liquid, and lyophilizing to prepare a phycocyanin finished product. The method is simple in extraction process and low in cost, and is suitable for intermittent and scale production processing of high-purity and high-yield spirulina phycocyanin finished product. The phycocyanin coarse extract can be stored for a long time, and the phycocyanin finished product which is prepared by further purification has excellent antioxidant free radical scavenging effect and fluorescent strength.

The invention relates to a biological sludge treatment method, which belongs to a biological sludge quantitative reduction method in the water treatment field. The aeration treatment is implemented onresidual sludge before the cell disruption. The time of the aeration treatment is 15-300 minutes; and dissolved oxygen of the sludge is 0.3-4.5mg / l. The invention has the advantages that the optimalaeration is implemented on settling sludge before the disruption under the condition of lacking nutrients. And the sludge is condensed before and after the aeration. The character of the sludge can berapidly changed through small amount of aeration. The following disruption becomes easy. The disruption effect is improved and the disruption cost is lowered. The following cost is greatly lowered with small amount of investment. For example, the ultrasonic disruption under the alkaline condition can save 35 percent of the alkaline using amount. And through the hydrolysis after the disruption, the biological decomposability is increased, which provides conditions for ensuring the effluent quality.

A method of separating and purifying nucleic acids from samples containing cells, such as blood and culture solutions. According to the method of the invention, a cell extract obtained by cell disruption is adsorbed by a filter made of a nonwoven fabric and the nucleic acid is eluted after washing the filter. Alkaline conditions of pH 12 or higher may be employed for elution of the nucleic acid, or the filter-adsorbed nucleic acid may be eluted by treatment with active oxygen or by using a surfactant. Nucleic acids separated and purified by the method of the invention can be used in nucleic acid amplification and nucleic acid sequence analysis techniques.

The invention provides a method for detecting microalgae oil, belonging to the field of oil detection. The method solves the technical problems that the needed sample is large and the consumed time is long in the existing weighing method. The method comprises the following steps: 1, diluting microalgae culture solution to obtain diluent; 2, detecting the fluorescence intensity of the dyed diluent; 3, centrifuging the diluent, and detecting the fluorescence intensity of the dyed supernate; 4, detecting the fluorescence intensity of the undyed diluent; and 5, computing the maximum difference of the fluorescence intensity, and computing the content of the microalgae oil according to a fluorescence intensity-oil content standard curve. The method is applied to detecting the unicellular algae such as the chlorella, the scenedesmus, the spirulina, the diatom, the dinoflagellate, the chrysophyceae, the euglenophyta, the stonewort and the like, and is applied to detecting the macro algae which is preprocessed by cell discruption.

The invention relates to SOD cell repair liquid and a preparation method thereof. The repair liquid contains superoxide dismutase (SOD), aloe juice, hyaluronic acid solution, alpha-lipoic acid and vitamin E; the weight ratio of the aloe juice, the hyaluronic acid solution, the alpha-lipoic acid to the vitamin E is 200-650 : 100-750 : 0.03-0.1 : 0.5-4; every 100g of the repair liquid contains 40000-4000000 activity unit of SOD; the preparation method is as follows: taking cactus stems and carrying out cell disruption, ultrafilter concentration and multistage precipitation and centrifugal separation process on the cactus stems to obtain crude enzyme supernatant; carrying out three-stage purification process and vacuum drying and grinding on the crude enzyme supernatant to obtain SOD freeze-dried powder; taking aloe plant leaves and carrying out degradation, decoloration, centrifugal separation process and filtration on the aloe plant leaves to obtain the aloe juice; blending the raw materials (components) according to the weight ratio for preparation to obtain the SOD cell repair liquid. The invention has the function of oxidation resistance, can alleviate physical fatigue and strengthen immunity of human body.

The invention discloses a production technique of low-temperature pressed rapeseed oil, wherein rapeseeds are cleaned, subjected to impurity removal, subjected to wall breaking based on freezing treatment and then fried to 60-70% rape to contribute to the oil leaching, a pressing technique is utilized in the invention, and a specially treated adsorbent is used for decoloring. A technique of heating after freezing is utilized in the invention to facilitate the cell disruption of the rapeseeds so as to improve the oil extraction rate, and the oil extraction rate is 5-6% higher than that of a traditional pressing method; and the oil is extracted by the pressing technique in the invention, so that no pollution exists, the purity of the oil product is guaranteed, and the oil is all natural and meets the demands of green food.

The invention discloses a giant salamander functional food with an immune regulation action and a preparation method thereof. The giant salamander functional food is prepared by obtaining giant salamander enzymatic hydrolysis powder by carrying out treatment of cell disruption, enzymatic hydrolysis and spray drying on giant salamander meat and then mixing the giant salamander enzymatic hydrolysis powder with extractive powder of medical and edible dual purpose plants including medlar, jujube and hawthorn and auxiliary materials according to a certain proportion. The preparation method keeps the nutrition activity of the giant salamander meat to the maximum extent, enhances the nutrition utilization ratio of a human body and also has the advantages of simple preparation process, convenient operation, safety, reliability, low production cost, and the like. The giant salamander meat functional food has the effects of eliminating oxygen free radicals, delaying aging and enhancing the immunity, is combined with the nourishing function of other medical and edible dual purpose plants so as to enhance the resistant ability of the human body to various diseases through long-term eating, strengthen the adaptability of the human body to the environment and be beneficial to the recovery of a patient after a surgery or with a weak constitution and is an ideal functional food.

The invention provides a method for preparing a radar wave-absorbing composite material based on a carbon nanometer film, comprising the following steps of: mixing carbon nanotubes and an anion surface dispersant, grinding the mixture in a mortar, pouring the grinded mixture into a beaker, and adding plasma water; dispersing and defoaming an obtained mixed solution in a magnetic stirrer; adding graphene oxide into plasma water to preparing a solution, and ultrasonically dispersing; mixing a carbon nanotube solution and the graphene oxide solution, centrifuging the mixture after ultrasonically dispersing by an ultrasonic cell disruption instrument, and compacting and solidifying residue after vacuum pumping filtration of a supernatant of the carbon nanotube and graphene oxide solution; heat-treating; adding a carbon nanometer film into an acetone weak solution of a resin for pre-infiltrating, and then drying; and adding the carbon nanometer filmed with layer pre-infiltration into an innermost layer of an aluminium alloy mould, laying a layer of carbon fiber / resin pre-infiltrated material in the medium, locking the mould, and molding by mould compression. A radar wave reflection rate of the radar wave-absorbing composite material based on the carbon nanometer film in a frequency range from 8GHz to 18GHz is lower than -10- -20dB.

The invention belongs to the field of microalgae biotechnologies, and particularly relates to a method for synthesizing astaxanthin by inducing chlorella vulgaris by using plant hormones and iron ions. The method specifically comprises the steps of: activating and culturing the chlorella vulgaris in a liquid culture medium to obtain a synchronously growing chlorella vulgaris solution for later use; and taking the chlorella vulgaris solution growing to an exponential growth phase, adding the plant hormones and the iron ions in the chlorella vulgaris solution, replenishing a carbon source and a nitrogen source for culturing, ending the culturing until the accumulation of the astaxanthin in chlorella vulgaris cells is maximum (the accumulation of the astaxanthin is determined by adopting a liquid chromatography during sampling), and harvesting cell disruption and extracting the astaxanthin. The method is simple and easy to operate and low in cost, and is capable of remarkably increasing the yield of the astaxanthin, thereby greatly increasing the efficiency of producing the astaxanthin by the chlorella vulgaris.

The invention provides a method for extracting dihydromyricetin and polysaccharide in vine tea at a relatively low temperature of 40-60 DEG C. The method for extracting dihydromyricetin and polysaccharide in vine tea is realized through the following technological means and technological processes: drying stems and leaves of vine tea, grinding, sieving, carrying out treatment for a certain time at a certain temperature and a certain ultrasonic power while water is taken as a solvent, carrying out natural cooling, then standing for 12-24 hours to crystallize and precipitate dihydromyricetin, centrifuging a feed liquid mixture, concentrating the obtained supernate, carrying out ethanol precipitation, washing and drying, so as to obtain crude polysaccharide of the vine tea; adding boiling water into residue after centrifugation, stirring, and insulating for about 10 minutes so as to ensure that crystallized dihydromyricetin is redissolved, then centrifuging immediately, and after the supernate is cooled, carrying out standing for 12-24 hours at 4 DEG C so as to ensure that dihydromyricetin is crystallized; centrifuging, and drying precipitate, so that dihydromyricetin crude extract is obtained. By adopting the method for extracting dihydromyricetin and polysaccharide in vine tea, the solubilizing effect of ultrasonic wave and the cell disruption effect are utilized for realizing rapid, efficient and synchronous extraction of two active substances in the vine tea at a low temperature, the yield of the dihydromyricetin and polysaccharide products is high, properties of the crude extract are good, and subsequent purification steps are effectively reduced, so that the energy consumption and the cost are reduced.

The invention relates to the cell disruption of microbes and the preparation of the microbe proteins for mass spectrometric analysis. The cells of microbes from microcolonies are disrupted by physical or chemical means directly on the nutrient medium. The released proteins are then transferred to sample supports by direct contact with their contact surfaces; electrophoresis can be used for assistance. Once the the proteins are firmly adsorbed on the contact surfaces, they can be washed with water in order to remove substances which interfere with the ionization process. For analysis by matrix-assisted laser desorption (MALDI), the proteins are prepared on the contact surfaces of the sample supports with matrix substances to form MALDI samples; the sample supports are then introduced into a MALDI mass spectrometer for the acquisition of the mass spectra. The microbes are identified by similarity comparisons between the mass spectra of the microbe proteins and similarly obtained reference spectra.

The invention provides a method for preparing mesoscale flaky vermiculite and high temperature polymer thermal insulation composite membrane. The invention adopts a technical proposal that primary mineral of vermiculite are ground to choose the part of vermiculite with a grain diameter less than 10 mu m; quaternary alkylammonium salt compound with a carbon chain of between 12 and 36 is used to modify the chosen vermiculite by organic expansion; a dispersing agent, grinding and a high-pressure homogenizer are adopted to treat the mesoscale flaky vermiculite; inorganic flaky vermiculite microcrystals are dispersed in organic high molecular polymer materials by using high temperature resistance polymer materials of poly(aryl ether sulfone) and poly(phthalazinone ether sulfone ketone) as continuous phrases and by methods of ultrasonic cell disruption, etc., and are formed into membranes by dry and wet methods. The products made by the method have extremely low heat conductivity, and excellent thermal insulation performance. Meanwhile, the composite membrane can be tightly covered on the surfaces of polysulfonamide and other fiber fabrics to improve the fireproof and thermal insulation properties, particularly the thermal insulation property of the fiber fabrics, can be used to make fire-protection clothing for safe access to fire ground or used as surface material for combat uniform.

The invention discloses a preparation method for small-particle-size magnetic agarose microspheres. According to the preparation method, emulgator and hydrophobic organic solvent serve as an oil phase, mixed liquor of agarose and superparamagnetic Fe3O4 aqueous solutions serves as an aqueous phase, the aqueous phase is added into the oil phase under mechanical stirring to carry out pre-dispersion, then ultrasonication is carried out on pre-dispersion emulsion, and cooling, magnetic separation and purification are carried out on the crushed emulsion to obtain the small-particle-size magnetic agarose microspheres. The magnetic agarose microspheres prepared according to the preparation method have high magnetic response and superparamagnetism, and compared with commercially available products, the magnetic agarose microspheres have smaller particle sizes, larger specific surface areas, more active sites, and broad application prospects. Due to the fact that hydrophilic magnetic cores are adopted, through the modes of vortex vibration, ultrasonic dispersion, microwave heating, mechanical stirring pre-dispersion, ultrasonication carried out by a cell disruption instrument and the like, not only can the fact that the magnetic cores can be uniformly dispersed in the agarose solutions be guaranteed, but also the fact that the obtained magnetic agarose is small in particle size is guaranteed, and distribution is narrow.

The invention discloses lactobacillus rhamnosus NX-2 and an application thereof in preparation of uric acid reducing drugs, and belongs to the technical field of microorganisms. The lactobacillus rhamnosus NX-2 disclosed by the invention has a preservation number of CGMCC No.20110. Non-inactivated and inactivated fermentation supernate, bacterial suspension and cell disruption material supernate of the lactobacillus rhamnosus NX-2 have uric acid reducing effects on a zebrafish high-uric-acid model, and can inhibit the activity of xanthine oxidase in the body of a high-uric-acid zebrafish. Thelactobacillus rhamnosus NX-2 disclosed by the invention has a huge potential application prospect in the aspect of preparing medicines for treating and / or preventing hyperuricemia.

The invention belongs to the technical field of medicines, and relates to a method for extracting astaxanthin from haematococcus pluvialis. The method comprises the steps of culturing a seed stock solution in a glass apparatus with a drainage system, and carrying out later amplification culture of the haematococcus pluvialis and accumulation culture of the astaxanthin after 10-15 d; centrifuging a culture in an exponential growth period of seed culture; and inoculating cell clusters in a BBM basal medium to obtain a primary culture. The later accumulation culture of the astaxanthin is amplification culture by using a breathable plastic bag type simple device provided by the invention. In the accumulation stage, a stress culturing method is adopted to obtain a lab-scale test haematococcus pluvialis culture; and haematococcus pluvialis powder is obtained by spray drying. According to a preparation technology that extracts astaxanthin from the haematococcus pluvialis by adopting an ultrasonic cell disruption assisted mixed solvent extraction method, the haematococcus pluvialis powder is added in an organic solvent to carry out ultrasonic cell disruption, and then the astaxanthin is obtained by the steps of reflux extraction in a water bath, suction filtration, filtrate merging and concentration. Compared with a conventional direct extraction method, the method provided by the invention saves extraction time, and increases astaxanthin yield.

The invention discloses a preparation method of oxidation-modified thermal cracking carbon black, belonging to the technical field of carbon material preparation. The preparation method comprises the following steps: with thermal cracking carbon black as a raw material, calcining at high temperature; carrying out carbon black oxidization by taking KMnO4, NaNO3 and concentrated H2SO4 as oxidants; carrying out suction filtration at reduced pressure on the reaction system before oxidation reaction; and then carrying out posttreatment on the oxidized carbon black by using ultrasonic or cell disruption. According to the preparation method, graphene microcrystals are peeled from the surface of the carbon black by controlling the calcining time, the calcining temperature, oxidation conditions and the like to destroy the regularity degree of the graph microcrystals on the surface of the thermal cracking carbon black, and thus the reaction activity and the adsorption capability of the thermal cracking carbon black are improved.

The invention relates to a health preparation rich in lutein and Beta-carotene, which is fruit pulp or fruit powder prepared with 50% to 90% of carrot and 10% to 50% of fresh petals of marigold in weight ratio, or capsule or tablets prepared further with the fruit powder. The health preparation is characterized in that, considering the preparation method, as the Beta-carotene in carrot and the lutein in marigold are fat-soluble vitamin and significantly different from the plant cytoderm in mass, both the Beta-carotene and the lutein are mixed and extracted centrifugally after cell disruption, thereby, the lutein and the Beta-carotene are enriched in the extracted fruit pulp economically and effectively, and allow to be directly used for development and utilization of functional food. The health preparation has the advantages that the combined medicinal function of natural plants of marigold and carrot is fully functioned; experiments prove the combination of Beta-carotene and the lutein enables to enhance the activity of superoxide dismutase in blood, compared to taking fruit pulp of Beta-carotene or fruit pulp of lutein individually, the health prevention effect is improved obviously.

A method of microbial cell disruption for use in making an aquaculture feed composition is disclosed, wherein a microbial biomass having a moisture level less than 10 weight percent and comprising oil-containing microbes is disrupted, resulting in a disruption efficiency of at least 30% of the oil-containing microbes to produce a disrupted microbial biomass, and, the disrupted microbial biomass is mixed with at least one aquaculture feed component to form an aquaculture feed composition.

The present invention relates to a method for preparing high-purity laver phycoerythrin by one-step chromatography, belonging to the preparation technology of the functional ingredients of halobios. Laver is swelled in PBS (1mM, pH6.8) buffer solution with a volume fifty times larger than the volume of the laver and smashed, then 800 watts of ultrasonic waves carry out cell disruption for 800 seconds to produce crude extract, which is precipitated by ammonium sulphate with 45 percent of saturation, microfiltrated via 0.1Mu m of film, ultrafiltered via a 10kDa film, goes through reversed-phase precipitation by ammonium sulphate with 20 percent of saturation and is precipitated and crystallized by ammonium sulphate with 65 percent of saturation under the temperature of 4 DEG C, and one week later, the crude extract passes through a DEAE cellulose-52 anion-exchange chromatography column, is gradiently eluted by PBS (1mM, pH6.8) buffer solutions with different concentrations of NaC1, separated and purified to produce the laver phycoerythrin. The purity (A562nm / A280nm) reaches 5.24, which accords with the reagent-grade requirement.

The present invention provides methods, compositions and diagnostic kits for the detection of Staphylococcus Aureus (SA) and antibiotic resistant forms and variants thereof, such as methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Staphylococcus aureus (VRSA), mupirocin-resistant Staphylococcus aureus (mupSA), and the like, in a sample population. The invention preferably involves the improvements of bacterial sampling by means of SA enrichment, followed by SA cell disruption and amplification procedures incorporating the use of multiplex assays for SA specific genes, such as mecA and coagulase negative Staphylococci (CONS) specific genes such as tufA, for SA identification and identification of its known species. This provides means for controlling for the thirty or more known CONS species in assessing SA samples, especially those CONS species that may carry antibiotic resistance genes, such as SCCmec.

The invention provides a method for wet extraction of purified microalgae oil. The method comprises the following steps: (1) adding an ethanol solution into ooze of collected microalgae, quickly stirring the mixture uniformly, carrying out centrifugation, and collecting sediments to obtain dehydrated algae ooze; (2) adding non-toxic or slightly-toxic organic solvent of a water solution of the organic solvent into the dehydrated algae ooze obtained in the step (1), carrying out cell disruption on the algae ooze, and extracting coarse oil in the microalgae; (3) carrying out centrifugation, collecting the coarse oil obtained in the step (2), adding water and inorganic salt into the coarse oil, stirring the mixture uniformly to form a coarse oil-water solution, adding n-hexane into the coarse oil-water solution, carrying out standing layering or centrifugal layering after uniform mixing to form three layers which are the upper solution layer, the middle solid matter layer and the lower solution layer, and collecting the solution on the upper layer; (4) carrying out desolvation on the solution on the upper layer to obtain the microalgae oil. According to the method for wet extraction of the purified microalgae oil, the yield of esterified grease is high, operation is easy, and energy consumption is low.

An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.

The invention provides a system integration technology through cell disruption, slurry extraction, membrane separation and macroporous resin adsorption and chromatography separation and purification, high temperature enzyme deactivation and spray drying and the like, which achieves a technical process that from the time fresh cistanche raw materials are fed until obtaining active matters such as monomeric compounds which are rich in echinacoside and verbascoside and the like in 30 to 80 minutes, and the invention effectively overcomes a technical defect that active components such as benzyl carbinol glycosides compounds are normally seriously degraded and lost, which are caused by an enzymatic reaction which is initiated by an imperfect dry treatment method of cistanche, and the invention obviously improves the medical value of cistanche. In the active maters of cistanche which are obtained ( calculating by dry matter), the productivity of echinacoside monomeric compound reaches to 9.8% to 19.6%, and the yield of verbascoside monomeric compound reaches to 2.0% to 7.5%. The content of echinacoside monomeric compound in active matters of cistanche which are obtained reaches to 15% to 49.6%, and the content of verbascoside monomeric compound reaches to 5% to 26.7%.

The invention relates to a method for preparing pyruvic oxidase. The method comprises the steps as follows: (1), cultivation of microbial cells containing the pyruvic oxidase, centrifugal collection of bacterial cells, and cell disruption; (2), preparation of a polyethylene glycol / inorganic salt two-aqueous phase extraction system solution; (3), three times of extraction separation by the aid of a two-aqueous phase extraction system, and obtaining of an inorganic salt solution of the pyruvic oxidase; and (4), flirtation and concentration of an ultrafiltration membrane, freezing and drying of a trapped fluid, and preparation of a pyruvate oxidase product. The method can prepare the pyruvic oxidase continuously in a large scale, the preparation technology of the oxidase is simple and convenient, the production period is short, the operation condition is mild, the recovery rate of the oxidase activity is high, and the production cost of the pyruvic oxidase is lower.

The present invention relates to a method for enhancing the yield of recombinant protein produced in genetically transformed plants. The invention most particularly relates to a method for preventing the undesirable proteolysis of recombinant proteins after harvest of the plant, during processing of the products from the plants. Especially, this invention focuses on introducing protease inhibitors in plants to prevent undesirable proteolysis of recombinant proteins at the time of cell disruption during the extraction process.

The invention relates to a method for extracting water-soluble carotenoid from carrots, tomatoes, red peppers, marigold and the like through enzymatic hydrolysis. The method comprises the following steps: adding water to fresh plant materials to obtain homogenate; carrying out ultrasonic cell disruption to the homogenate; mixing the homogenate with cellulase and pectinase and stirring the mixture while adding surfactant to the mixture; performing enzymatic hydrolysis; filtering the hydrolyzed mixture with a filter; and drying the filtrate in vacuum to obtain the water-soluble carotenoid.