CDKN2A gene fragment and application of primers of CDKN2A gene fragment in diagnosing tumors
A gene fragment and tumor diagnosis technology, applied in the field of genes, can solve the problem of undetermined CDKN2A gene fragment, etc., and achieve the effect of wide application, cost reduction, and easy operation.
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[0020] The present invention will be further illustrated by specific examples of the methylation detection of CDKN2A gene fragments in the blood of tumor patients and normal people.
[0021] 1) Genomic DNA extraction and bisulfite treatment.
[0022] The DNA extraction kit was used to extract the total genomic DNA of the blood sample, and the purity and concentration of the DNA solution were detected by agarose gel electrophoresis and ultraviolet-visible light spectrophotometry. Take a certain amount of genomic DNA with a known concentration and treat it with a bisulfite conversion kit to convert unmethylated cytosine (C) into uracil (U), while methylated cytosine remains constant.
[0023] 2) The following describes the specific design method of PCR primers for CDKN2A gene fragments of the present invention as follows:
[0024] Download the full-length human CDKN2A gene sequence from the nucleic acid sequence database Genbank (http: / / www.ncbi.nlm.nih.gov / genbank / ) (sequence registra...
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