Preparation method for polyclonal antibody to salmonella effect protein SopB
A polyclonal antibody and effector protein technology, applied in the biological field, can solve problems that have not yet been reported in research, and achieve the effect of avoiding non-specific cutting
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[0045] biomaterials
[0046] Salmonella enterica subsp.enterica serovar Typhimurium LT2 (Salmonella enterica subsp.enterica serovar Typhimurium) is deposited in the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microbial Cultures. , the date of deposit is December 20, 2012, and the deposit number is CGMCC No.7020.
[0047] The pET28a expression vector was purchased from Novagen; Escherichia coli DH5α and Escherichia coli BL21(DE3) strains were purchased from Promega; restriction enzymes NdeI, XhoI, BamHI, T4 DNA ligase, Taq DNA polymerase and bacterial genomic DNA extraction kit All were purchased from Takara Company; pSUMO expression vector, pGEX-6P-2 expression vector, HiTrap Q HP anion exchange column, Superdex20010 / 300 molecular sieve and HiTrap NHS-activated HP affinity chromatography column were purchased from GE Healthcare; trishydroxymethylamino Methane, acrylamide, kanamycin, ampicillin, methylene bisacrylamide, sodium chloride, imi...
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